A method for preparing silk fibroin peptide by hydrolyzing silk fibroin with serrapeptase
A serrapeptase and silk fibroin peptide technology, applied in the field of biochemical industry, can solve the problems of low silk peptide activity and low hydrolysis efficiency, and achieve the effects of fast hydrolysis speed, easy industrial application, and simple method
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Embodiment 1
[0029] Silkworm cocoons were used as raw materials to prepare silk fibroin, and eight kinds of proteases were selected to hydrolyze silk fibroin to prepare silk fibroin peptides, and the proteases with high degree of hydrolysis and antioxidant activity were screened.
[0030] (1) Preparation of silk fibroin: take by weighing 5g of silkworm cocoons cut into pieces, add to 200mL mass concentration of 0.5% Na 2 CO 3 In an aqueous solution, boil for 30 minutes, then rinse with a large amount of tap water, and repeat the boiling and rinsing twice. Wash the silk after 3 times of degumming with distilled water, and dry it in an oven at 85°C to obtain 3.46g of silk fibroin after degumming, and the yield of silk fibroin is 69.2%; add 3.46g of silk fibroin to 70mL of 40% CaC1 2 Aqueous solution, boiled for 5 minutes, after the silk fibroin dissolves, filter through 8 layers of gauze to obtain a silk fibroin solution, the silk fibroin concentration is 45.5g / L;
[0031] (2) Screening o...
Embodiment 2
[0042] (1) Preparation of silk fibroin: take by weighing 5g of silkworm cocoons cut into pieces, add to 200mL mass concentration of 0.5% Na 2 CO 3 In an aqueous solution, boil for 30 minutes, then rinse with a large amount of tap water, and repeat the boiling and rinsing twice. Wash the silk after 3 times of degumming with distilled water, and dry it in an oven at 85°C to obtain 3.27g of silk fibroin after degumming, and the yield of silk fibroin is 65.4%; add 3.27g of silk fibroin to 75mL of 40% CaC1 2 Aqueous solution, boiled for 5 minutes, after the silk fibroin dissolves, filter through 8 layers of gauze to obtain a silk fibroin solution, the silk fibroin concentration is 40.5g / L;
[0043] (2) The degree of hydrolysis and antioxidant activity of silk fibroin peptides prepared by hydrolyzing silk fibroin with serrapeptase at different times: Take 6.2 mL of silk fibroin solution prepared in step (1) and add Tris-HCl with pH 7.5 and 0.5 mol / L Buffer to a total volume of 50...
Embodiment 3
[0049] Using silkworm cocoons as raw materials, using serrapeptase to hydrolyze silk fibroin to prepare silk peptides, the following steps can be followed:
[0050] (1) Take by weighing the silkworm cocoon that 5g is cut into pieces, join in 200mL mass concentration and be 0.5% Na 2 CO 3 In an aqueous solution, boil for 30 minutes, then rinse with a large amount of tap water, and repeat the boiling and rinsing twice. Wash the silk that has been degummed three times with distilled water, and dry it in an oven at 85°C to obtain 3.35 g of silk fibroin after degumming;
[0051] (2) 3.35g silk fibroin prepared by step (1) is added to 75mL mass concentration of 40% CaCl 2 Aqueous solution, boiled for 5 minutes, after the silk fibroin dissolves, filter through 8 layers of gauze to obtain a silk fibroin solution, the silk fibroin concentration is 40.2g / L;
[0052] (3) Take 6.2 mL of silk fibroin solution prepared in step (2), add pH 8.0, 0.5 mol / L Tris-HCl buffer solution to a tota...
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