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Method for preparing functional protein polypeptide by using bionic method

A technology of functional protein and bionic method, which is applied in the direction of fermentation, etc., can solve the problems of insufficient degradation of antigenic protein content, insufficient digestion and absorption rate, and unstable quality of fermented soybean meal, so as to enhance the immunity of piglets and disease resistance, good social and economic benefits, and low production costs

Pending Publication Date: 2019-04-16
GUANGZHOU WISDOM BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent applications CN1786182A and CN101558818A mention the use of fermentation to prepare soybean meal with low anti-nutritional factors and high conversion rate. However, due to the disadvantages of difficult control of the process, the quality of fermented soybean meal is unstable, the content of functional small peptides is low, and the antigenic protein is degraded. Incomplete, etc.; CN1911066B also has the problem that the quality of the above-mentioned products is not easy to control; CN106578421A is prepared by enzymatic hydrolysis, the process is highly controllable, and the product is relatively clear, but its product is mainly polypeptide, and the content of antigenic protein is not fully degraded. Inadequately maximized digestion and absorption
At present, there is no plant protein peptide product with low anti-nutritional factors, rich in functional small peptides and high digestibility produced by bionic method in the market

Method used

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  • Method for preparing functional protein polypeptide by using bionic method
  • Method for preparing functional protein polypeptide by using bionic method
  • Method for preparing functional protein polypeptide by using bionic method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Get 500kg of defatted and dehulled soybean meal raw materials, pre-crumble it through a 60-mesh sieve, add water (the ratio of soybean meal raw material to water is 1:5) and stir evenly, and prepare a soybean meal mixture with a solid content of 15%; Value is adjusted to 3.5, add compound protease A (addition is 3% of plant protein solid matter amount, papain (addition is 1% of plant protein solid matter amount) and bromelain (addition amount is plant protein solid matter amount 1%) 1.5%), at 50°C, enzymatically hydrolyze for 6 hours, raise the temperature of the enzymatic hydrolyzate to 85°C, and inactivate the enzyme for 20 minutes to obtain a primary soybean meal enzymatic hydrolyzate; adjust the pH value of the primary soybean meal enzymatically hydrolyzate to 7, add compound protease B (addition is 3.5% of vegetable protein solid matter quality), lysine aminopeptidase (addition is 3% of vegetable protein solid matter quality) and carboxypeptidase (addition is 5% of ...

Embodiment 2

[0038] The difference between this embodiment and above-mentioned Example 1 is: 1) get 300kg rice protein raw material, rice protein and water weight ratio are 1:6, the amount of the compound protease A added is 2%, 0.05% papain and 0.5% % bromelain, at a pH of 4.5 and a temperature of 40°C, hydrolyze for 5 hours; 2) Enzyme inactivation at high temperature: after one enzymolysis, heat up to 70°C and maintain for 30 minutes; 3) The added complex protease B The amount is 5%, 0.6% lysine aminopeptidase and 2% carboxypeptidase, and the pH is 8, and the temperature is 45°C, and the enzymolysis is carried out for 6h; 4) the obtained secondary protein hydrolyzate is After homogenizing for 60 minutes, carry out high-speed centrifugal spray drying under the condition that the air inlet temperature is 200°C and the air outlet temperature is 70°C.

[0039] In this embodiment, the product quality is stable, and the content of active small molecule peptides (19% for those with a relative m...

Embodiment 3

[0041] The difference between this embodiment and above-mentioned embodiment 1 is: 1) get peeled soybean meal, cottonseed meal and rapeseed meal (1:1:1) raw material 150kg, raw material and water weight ratio are 1:7, the compound protease added The amount of A is 4%, 0.7% papain and 1% bromelain, and the pH is 5.5, and the temperature is 45°C, and the enzymolysis is carried out for 4 hours; ℃, maintained for 10 minutes; 3) the amount of compound protease B added was 7%, 1% lysine aminopeptidase and 4% carboxypeptidase, and the pH was 8 and the temperature was 60 ℃. Decompose for 3 hours; 4) After homogenizing the obtained secondary protein enzymatic hydrolyzate for 50 minutes, perform high-speed centrifugal spray drying under the condition that the air inlet temperature is 220°C and the air outlet temperature is 80°C.

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Abstract

The invention relates to a method for preparing functional protein polypeptide by using a bionic method, which uses natural plant protein as a raw material, utilizes non-animal-derived protease and simulatesan animal digestion process to produce enzymolysis protein polypeptide. The method comprises the following steps of: pre-crushing plant protein raw materials, adding water for preparation, performing primary enzymolysis by adopting non-animal-derived composite protease A, papain and bromelain, adding composite protease B, lysine aminopeptidase and carboxypeptidase for secondary enzymolysisto obtain secondary enzymolysis protein slurry, then homogenizing, spray-drying, and sterilizing to obtain the final product. The method has the advantages of simple process flow, low cost, high content of active micromolecular peptide (the relative molecular weight is between 186 and 1000Da) and free amino acid (186Da) of the prepared product, complete degradation of anti-nutritional factors, invitro digestion absorption rate of more than or equal to 95%, and capability of effectively replacing high-quality animal-derived protein, the growth of young animals isobviously promoted and the method has good social and economic benefits.

Description

technical field [0001] The invention relates to a method for preparing functional protein polypeptides by a bionic method, and belongs to the technical field of deep processing of plant proteins such as soybean meal protein, cottonseed protein, pea protein or rice protein. Background technique [0002] Soybean meal protein, cottonseed protein, rapeseed meal, pea protein or rice protein are by-products obtained from the processing of corresponding products, especially soybean meal protein, a high-quality plant protein resource, accounting for about 75% of soybean mass. At present, in the world, soybean meal accounts for as high as 62% of all vegetable cakes used for feeding, becoming the most important vegetable protein feed raw material. However, due to the complex and dense three-dimensional structure of the above-mentioned plant proteins and the presence of anti-nutritional factors such as trypsin inhibitors, non-starch polysaccharides (NSP), antigenic proteins, and allerg...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 闫方权吕勇陈卫吴世林
Owner GUANGZHOU WISDOM BIO TECH
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