Method for enzymatic synthesis of penicillin V salt
A technology of penicillin V and enzymatic synthesis, which is applied in the field of medicine and chemical industry to achieve the effects of good product quality, low cost and short production cycle
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[0031] Example 1
[0032] Take 25.0g 6-APA, add 300mL pure water, drop 3mol / L ammonia solution to fully dissolve 6-APA, add 30.0g phenoxyglycol acetate, mix well, add 50.0g immobilized penicillin acylase , 3mol / L ammonia solution was added dropwise to make the reaction pH 5.0, the temperature was 30°C, and the reaction time was 90 minutes for sampling and HPLC detection. At this time, the concentration of 6-APA was 0.8 mg / mL and the concentration of penicillin V was 106.9 mg / mL; Separate the immobilized enzyme and the reaction solution, adjust the pH value of the reaction solution to 3.0 with 6mol / L hydrochloric acid, grow the crystals for 20 minutes, and filter to obtain a penicillin V acid filter cake; the filter cake is transferred to 5 times the weight of pure water and used Adjust the pH value of the mixed solution with a certain concentration of potassium carbonate solution to 6.0, then add n-butanol to a certain concentration, and concentrate by evaporation under reduced p...
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[0033] Example 2
[0034] Take 25.0g of 6-APA, add 400mL of pure water, add 3mol / L ammonia solution dropwise to fully dissolve 6-APA, add 30.0g of phenoxyglycerol, mix well, add 75.0g of immobilized penicillin acylase, dropwise Add 3mol / L ammonia solution to make the reaction pH 6.0, temperature 20°C, and react for 80 minutes to sample by HPLC. At this time, the concentration of 6-APA is 0.3 mg / mL, and the concentration of penicillin V is 87.5 mg / mL; the screen is separated and fixed. Chemical enzyme and reaction solution, adjust the pH value of the reaction solution to 2.8 with 6mol / L hydrochloric acid, grow crystals for 20 minutes, and filter to obtain penicillin V acid filter cake; the filter cake is transferred to 4 times the weight of pure water with a certain concentration Adjust the pH value of the mixed solution to 6.3 with potassium carbonate solution, then add n-butanol to a certain concentration, and concentrate by evaporation under reduced pressure until crystals are ...
Example Embodiment
[0035] Example 3
[0036] Take 25.0g 6-APA, add 400mL pure water, drop 3mol / L ammonia solution to fully dissolve 6-APA, add 35.0g phenoxybutyl erythritol, mix well, add 50.0g immobilized penicillin acylase , 3mol / L ammonia solution was added dropwise to make the reaction pH 5.0, temperature 30°C, reaction for 90 minutes, sampling HPLC detection, at this time 6-APA concentration is 0.5mg / mL, penicillin V concentration is 89.0mg / mL; Separate the immobilized enzyme from the reaction solution, adjust the pH value of the reaction solution to 2.8 with 6mol / L hydrochloric acid, grow the crystals for 20 minutes, and filter to obtain a penicillin V acid filter cake; the filter cake is transferred to 4 times the weight of pure water and used Adjust the pH value of the mixed solution with a certain concentration of potassium carbonate solution to 6.5, then add n-butanol to a certain concentration, and concentrate by evaporation under reduced pressure until crystals are precipitated. The cry...
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