Development and Application of SNP Marker of Rice Blast Resistance Gene pi2

Abstract

The present invention provides a SNP molecular marker K_060502 closely linked to the broad-spectrum rice blast resistance gene Pi2. The SNP marker detects that the base at the 10432612 site of rice chromosome 6 is G or T. The SNP developed based on KASP technology The labeled primer combinations are Primer X, Primer Y and Primer C. The present invention utilizes KASP technology to rapidly genotype the SNP markers closely linked to the broad-spectrum rice blast resistance gene Pi2, which can be applied in commercial molecular breeding with high, medium and low throughput; at the same time, the selection of SNP marker phenotypes is developed The efficiency reaches 100%, and it can quickly and accurately detect the broad-spectrum rice blast resistance gene Pi2 in different germplasm resources such as indica rice and japonica rice; The pollution of sol and the use of toxic substances such as ethidium bromide (EB) can carry out molecular marker-assisted selection of prospects in the early stage of breeding, reduce the scale of field planting of breeding populations, reduce breeding costs, and speed up the breeding process.

Description

technical field [0001] The invention relates to the fields of molecular markers and crop breeding, in particular to the development and application of a SNP marker of rice blast resistance gene Pi2. Background technique [0002] Rice is one of the most important food crops in China and even in the world. More than half of the world's population uses rice as a staple food. Rice blast, known as the cancer of rice, is one of the most serious diseases to rice production. The hybrid rice widely promoted in China lacks the introduction of high-quality disease-resistant resources from distant sources, and the genetic diversity of the parents is not high. The improvement of disease resistance is a major bottleneck; in major rice-growing countries, there are often reports of rice blast epidemics and outbreaks. Yield losses of 20-100% can be caused annually. [0003] Practice has proved that breeding disease-resistant varieties is the most economical, safe, environmentally friendly ...

AI Technical Summary

Problems solved by technology

[0006] Currently developed molecular markers generally require agarose gel electrophoresis (Deng Guofu et al., CN105462971 B; Zhang Mingyong et al., CN106636358 A), and even more complex polyacrylamide gel electrophoresis (Chen Qingquan et al., CN 102312000 A Chen Songbiao, etc., CN 104073487 B; Ji Jianhui, etc., CN 104450932 B; Sheng Zhonghua, etc., CN106636395 A), some also need to carry out polyacrylamide gel electrophoresis after enzymatic digestion (Hua Xia, etc., CN 103320437 B), the operation is loaded down with trivial details, Time-consuming and material-consuming, not convenient for large-scale identification; Jin Mingzhen et al. (CN 107435068A) detect SNP (Single Nucleotide Polymorphism) sites by the method of high resolution melting curve analysis (High Resolution Melting Curve Analysis, HRM), although avoiding electrophoresis or Enzyme digestion process, but as the amplification product fragments increase, the accuracy and stability of the detection will gradually decrease

Images