Tobacco bio-organic fertilizer using natural extract waste residues as raw materials, preparation method and application of fertilizer
A bio-organic fertilizer and a technology for natural extracts, which are applied to the bio-organic fertilizer for tobacco using natural extract waste residue as raw materials and the fields of its preparation and application, can solve the problems of hindering the preparation of bio-organic fertilizer, hindering the growth and inactivation of microorganisms, etc. , to achieve the effect of benefiting growth and development, good economic and ecological benefits, and uniform size
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[0025] Below in conjunction with embodiment the present invention is further described.
[0026] The specific composite bacterial agent for crude fiber degradation in the following examples is prepared by the following method:
[0027] a. Cultivate Bacillus agave and Bacillus subtilis in bacterial culture medium respectively, and its culture condition is set as: cultivation temperature is 35 ℃, and rotating speed is 150rpm, and liquid filling volume is 50mL / 250mL, and cultivation time is 30h, The formulation of the bacterial culture medium is glucose 20g, soybean meal 90g, K2HPO4 3H2O 0.5g, MgSO4 7H2O 0.5g, MnSO4 H 2 O0.005g, distilled water 1000mL, pH 7.0-7.5;
[0028] b. solid-state fermentation culture of Aspergillus niger on bran with a water content of 50% for 48 hours, the temperature was set at 30°C, and the pH was natural;
[0029] c. After the cultivation is completed, mix the Aspergillus niger cultivated in the Bacillus agave, Bacillus subtilis and step b according...
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