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Printable composite hydrogel with high toughness, preparation method and application

A composite hydrogel technology with high toughness, applied in the field of biomedical engineering, can solve the problems that the production technology cannot realize individualized geometry, insufficient scaffold toughness, and inability to induce and regulate cell-specific differentiation phenotypes, so as to improve cell survival High efficiency, good dispersion, and the effect of improving mechanical strength

Active Publication Date: 2021-08-27
GUANGDONG PROVINCIAL BIOENGINEERING INST (GUANGZHOU SUGARCANE IND RES INST)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The primary purpose of the present invention is to provide a high-toughness hydrogel scaffold for the shortcomings of the lack of toughness of traditional hydrogel scaffolds, the inability to induce and regulate cell-specific differentiation phenotypes, and the shortcomings of fabrication techniques that cannot realize individualization and complex geometric shapes. Preparation method of printable composite hydrogel

Method used

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  • Printable composite hydrogel with high toughness, preparation method and application
  • Printable composite hydrogel with high toughness, preparation method and application
  • Printable composite hydrogel with high toughness, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] (1) Preparation of modified gelatin and modified cellulose nanocrystals:

[0063] Preparation of modified gelatin: Weigh 10 g of gelatin (Sigma company, G9382 derived from cowhide) and dissolve it in 100 mL of ultrapure water to prepare a gelatin solution with a mass concentration of 10%, then adjust the pH to 8.5 with 5 mol / L NaOH solution ; Add 7.5 mL of methacrylic anhydride dropwise, react at 40°C for 6 hours, and control the pH of the reaction solution at 8-9 through NaOH during the entire reaction process; place the product obtained by the reaction in a dialysis bag with a molecular weight cut-off of 3500 for dialysis for 3 days, freeze Dry to obtain modified gelatin.

[0064] Preparation of modified cellulose nanocrystals: Weigh 1 g of cellulose nanocrystals (purchased from Cellulose lab, Canada, containing 12% cellulose nanocrystal suspension, pore size distribution, 90-300nm, crystallization index 87%), add 0.25 g NaBr, 0.025 g TEMPO and 2.5 mL of a 12% wt NaC...

Embodiment 2

[0069] (1) The preparation of modified gelatin and modified cellulose crystals is the same as in Example 1.

[0070] (2) Preparation of composite hydrogel: 50mL of modified gelatin with a concentration of 8% by mass was prepared with ultrapure water, and the modified cellulose nanocrystals prepared in step (1) were added to make the final mass ratio 0.8% , add 60 mg of NHS, shake well, add EDC 120 mg, react at room temperature for 15 minutes, then transfer to a dialysis bag (molecular weight 3500) for dialysis for 5 days, freeze-dry, and store in a -20°C refrigerator for later use.

[0071] (3) Preparation of cell-loaded hydrogel: sterilize all materials under ultraviolet light for more than 1 h, dissolve the above-mentioned composite hydrogel in DMEM-F12 medium, and obtain a concentration of the composite hydrogel in solution A with a mass volume ratio of 8 %; add photoinitiator Irgacure 2959 (CIBA Chemicals company) solution in solution A, obtain solution B, wherein the fina...

Embodiment 3

[0074] (1) The preparation of modified gelatin and modified cellulose crystals is the same as in Example 1.

[0075] (2) Preparation of composite hydrogel: 50 mL of modified gelatin with a concentration of 8% by mass was prepared with ultrapure water, and the modified cellulose nanocrystals prepared in step (1) were added to make the final mass ratio 2.0% , add 150mg NHS, shake well, add EDC 300mg, react at room temperature for 15 minutes, transfer to dialysis bag (molecular weight 3500) for dialysis for 5 days, lyophilize, and store in -20°C refrigerator for later use.

[0076](3) Preparation of cell-loaded hydrogel: sterilize all materials under ultraviolet light for more than 1 h, dissolve the above-mentioned composite hydrogel in DMEM-F12 medium, and obtain a concentration of the composite hydrogel in solution A with a mass volume ratio of 8 %; add photoinitiator Irgacure 2959 (CIBA Chemicals company) solution in solution A, obtain solution B, wherein the final concentrati...

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Abstract

The invention provides a printable composite hydrogel with high toughness, a preparation method and application. In the present invention, modified gelatin is prepared by using monomers containing methacryloyl groups, and modified cellulose nanocrystals are obtained by using a catalytic oxidation system; the prepared modified gelatin and modified cellulose nanocrystals are mixed, Under these conditions, a printable composite hydrogel with high toughness is obtained. The modified cellulose nanocrystals and the modified gelatin form a stronger covalent bond and hydrogen bond double cross-linked network, which greatly improves the mechanical strength of the hydrogel, and at the same time makes the dispersion of the modified nanocrystals better. After that, it helps stem cells to differentiate into cartilage. Therefore, the composite hydrogel is suitable for preparing biomedical engineering materials for tissue repair.

Description

technical field [0001] The invention belongs to the technical field of biomedical engineering, in particular to a printable composite hydrogel with high toughness, its preparation method and application. Background technique [0002] Some soft tissues, such as cartilage tissue and adipose tissue, do not have the ability to regenerate because they do not contain or have few blood vessels and lymph nodes; and because their self-repair ability is very limited, when the soft tissue is damaged or missing, it cannot repair itself. Tissue-engineered scaffolds are an important alternative therapeutic measure that promises to finally address this issue. Gelatin-based hydrogel materials, which are conducive to the seeding and growth of seed cells in terms of biocompatibility, degradability, cell-material interface, three-dimensional porous structure and plasticity, are ideal tissue engineering matrix materials. Studies have confirmed that fibroblasts, chondrocytes, and osteoblasts ca...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/20A61L27/38A61L27/50A61L27/52C08J3/075C08L89/00C08L1/04
Inventor 马年方梁磊张佳泳曾建李锦荣黄玉南
Owner GUANGDONG PROVINCIAL BIOENGINEERING INST (GUANGZHOU SUGARCANE IND RES INST)
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