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Method and application of increasing the content of rare saponins in total saponins of ginseng using acetic acid bacillus whole cell catalysis

A technology of total ginsenosides and acetic acid bacteria, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low average enzyme activity of fermentation broth supernatant and broken cells, and reduce production costs and increase content. Effect

Active Publication Date: 2020-12-01
SOUTH CHINA INST OF COLLABORATIVE INNOVATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, our previous research found that Acetobacter acetobacter is a high-producing strain of β-glucosidase, and the enzyme produced is an intracellular insoluble membrane-bound enzyme, that is, only the whole cell shows high enzyme activity, and the fermentation supernatant and broken cells have enzyme activity. lower

Method used

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  • Method and application of increasing the content of rare saponins in total saponins of ginseng using acetic acid bacillus whole cell catalysis
  • Method and application of increasing the content of rare saponins in total saponins of ginseng using acetic acid bacillus whole cell catalysis
  • Method and application of increasing the content of rare saponins in total saponins of ginseng using acetic acid bacillus whole cell catalysis

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Experimental program
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Embodiment 1

[0062] 1. The preparation method of total ginseng saponins is as follows:

[0063] (1) Extraction method of total ginseng saponins: Raw sun-dried ginseng powder is passed through an 80-mesh sieve, decocted and extracted in an extraction tank, the mass ratio of ginseng powder to water is 1:8, the extraction temperature is 95°C, the extraction time is 1 hour, and the extraction times are 5 Once, the extracts were combined and concentrated under reduced pressure to 10% of the volume of the stock solution to obtain the ginseng extract.

[0064] (2) Preliminary purification of the macroporous resin of the ginseng extract: use a chromatographic column of 45mm*80cm to pack macroporous adsorption resin AB-8 with 60% column volume, apply the wet method, and use distilled water, 10% (w / w) ethanol solution and 20% (w / w) ethanol solution to wash away impurities, 30-95% (w / w) ethanol solution gradient elution, collect the eluate; then concentrate the eluate to 20% (v / v), to obtain total ...

Embodiment 2

[0080] 1. The preparation method of total ginseng saponins is as follows:

[0081] (1) Extraction method of total ginseng saponins: pass raw sun-dried ginseng powder through a 60-mesh sieve, extract in an extraction tank, the ratio of solid to liquid is 1:10, the extraction temperature is 95°C, the extraction time is 1.5 hours, the number of extractions is 4 times, and the extraction is combined solution, concentrated under reduced pressure to 15% of the volume of the stock solution to obtain a ginseng extract.

[0082] (2) Preliminary purification of ginseng extract macroporous resin:

[0083]Use a chromatographic column of 45mm*80cm, load 60% of the column volume macroporous adsorption resin AB-8, load the sample by wet method, use distilled water, 10% (w / w) ethanol solution and 20% (w / w) sequentially ) ethanol solution to wash away impurities, 30-95% (w / w) ethanol solution gradient elution, collect eluate; then eluate is concentrated to 15% (v / v), to obtain total ginseng s...

Embodiment 3

[0096] 1. The preparation method of total ginseng saponins is as follows:

[0097] (1) Extraction method of total ginseng saponins: pass raw sun-dried ginseng powder through a 40-mesh sieve, extract in an extraction tank, the ratio of solid to liquid is 1:15, the extraction temperature is 95°C, the extraction time is 1.5 hours, and the extraction times are 3 times. The extracts were combined and concentrated under reduced pressure to 20% of the stock solution to obtain a ginseng extract.

[0098] (2) Preliminary purification of the macroporous resin of the ginseng extract: use a chromatographic column of 45mm*80cm to pack macroporous adsorption resin AB-8 with 60% column volume, apply the wet method, and use distilled water, 10% (w / w) ethanol solution and 20% (w / w) ethanol solution to wash away impurities, 30-95% (w / w) ethanol solution gradient elution, collect the eluate; then concentrate the eluate to 25% (v / v), to obtain total ginsenosides extract.

[0099] 2. Acetobacte...

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Abstract

The invention discloses a method and application for improving the content of rare saponin in ginseng total saponin by whole cell catalysis of bacillus aceticus. The method comprises the following steps: (1) pulverizing and sieving white ginseng and then adding a solvent for extraction and concentration to obtain a ginseng extracting solution; (2) purifying the ginseng extracting solution with macroporous resin, and then concentrating the extracting solution to obtain a ginseng total saponin extracting solution; (3) fermenting bacillus aceticus by a high-density fermentation method to make thecell concentration in an obtained fermentation solution reach 1.5*109 cfu / mL or more, and then conducting filtering and concentrating by a tangential flow microfiltration system to obtain the concentrated fermentation solution; (4) adding the ginseng total saponin extracting solution to the concentrated fermentation solution for biotransformation to obtain an aqueous solution of the ginseng totalsaponin containing the rare saponin. The method has mild conditions and can greatly increase the content of the rare saponin in the ginseng total saponin extracting solution, and high application value can be achieved.

Description

technical field [0001] The invention belongs to the field of biotransformation, and in particular relates to a method and application for increasing the content of rare saponins in total ginsenosides catalyzed by whole cells of Acetobacter. Background technique [0002] Ginsenoside is the main active ingredient of ginseng, which has physiological functions such as improving memory, improving blood circulation, anti-tumor, and improving immunity. The content of rare ginsenosides in ginseng is very small, but their pharmacological effects are stronger than those of the constant components, especially their antibacterial, anti-inflammatory and anti-tumor effects are increasingly concerned. Ginsenosides are mainly composed of terpenoids as the mother nucleus and oligosaccharides as ligands. When the body ingests ginsenosides, due to its large molecular weight, strong polarity, and weak mucosal penetration, it is poorly absorbed in the gastrointestinal tract. The bioavailability...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/00C12P33/20C12R1/02
Inventor 夏晚霞杨继国任杰王云刘玮刘丹
Owner SOUTH CHINA INST OF COLLABORATIVE INNOVATION