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Method for detecting cecropin B by taking Fe3O4@Au as signal amplifier

A signal amplifier and cecropin technology, which is applied in biological testing and material inspection products, can solve problems such as the difficulty of determining the titration end point of potentiometric titration, the high cost of high-performance liquid chromatography, and the large impact of objective environment, etc., to achieve objective environmental protection. The effect of small influence, high detection sensitivity and fast detection speed

Inactive Publication Date: 2019-05-17
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing detection methods include potentiometric titration and high performance liquid chromatography, etc., and the potentiometric titration titration end point is difficult to determine, and is greatly affected by the objective environment; the analysis cost of high performance liquid chromatography is high, time consuming

Method used

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  • Method for detecting cecropin B by taking Fe3O4@Au as signal amplifier
  • Method for detecting cecropin B by taking Fe3O4@Au as signal amplifier
  • Method for detecting cecropin B by taking Fe3O4@Au as signal amplifier

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Experimental program
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Embodiment 1 4

[0051] Embodiments 1 to 4 Blank detection device and polarized light detection

Embodiment 1

[0053] 1) Cut the slide into upper and lower slides, then soak the upper and lower slides in Piranha solution at 80°C to generate hydroxyl groups on the surface of the upper and lower slides, and then pass through the ethanol and deionized water for cleaning, and then with N 2 Blow dry, wherein, Piranha solution H 2 SO 4 with H 2 o 2 The volume ratio is 7:3;

[0054] 2) Soak the upper glass slide in DMOAP aqueous solution for 40min, rinse with ultrapure water, and then wash with N 2 Blow dry; at the same time, place the lower slide in ethanol solution at 60°C for 1 hour, rinse with ultrapure water, and then wash with N 2 Blow dry, wherein, the volume ratio of TEM and DMOAP in the ethanol solution is 3:1. Wherein, the volume fraction of the DMOAP aqueous solution in the upper slide is 0.1%; the volume fraction of TEM in the lower slide is 5%, and the volume fraction of DMOAP is 1.7%;

[0055] 3) Add the cecropin B antibody solution dropwise to the surface of the lower gl...

Embodiment 2

[0058] 1) Cut the slide into upper and lower slides, then soak the upper and lower slides in Piranha solution at 90°C to generate hydroxyl groups on the surface of the upper and lower slides, and pass through ethanol respectively and deionized water for cleaning, followed by N 2 Blow dry, wherein, Piranha solution H 2 SO 4 with H 2 o 2 The volume ratio is 7:3;

[0059] 2) Soak the upper glass slide in DMOAP aqueous solution for 40min, rinse with ultrapure water, and then wash with N 2 Blow dry; at the same time, place the lower slide in ethanol solution at 75°C for 1 hour, rinse with ultrapure water, and then wash with N 2 Blow dry, wherein, the volume ratio of TEM and DMOAP in the ethanol solution is 3:1. Wherein, the volume fraction of the DMOAP aqueous solution in the upper glass slide is 0.2%, the volume fraction of TEM in the lower glass slide is 4.5%, and the volume fraction of DMOAP is 1.5%;

[0060] 3) Add the cecropin B antibody solution dropwise to the surface...

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Abstract

The invention discloses a method for detecting cecropin B by taking Fe3O4@Au as a signal amplifier. The method comprises the following steps: 1) acidifying an upper slide and a lower slide; 2) soakingthe upper slide in DMOAP, flushing the upper slide and drying the upper slide with air; meanwhile, soaking the lower slide in TEA / DMOAP, then flushing the lower slide, and drying the lower slide withair; 3) dripping a cecropin B antibody solution on the surface of the lower slide dropwise, performing incubation, flushing the lower slide and drying the lower slide with air; 4) dripping a Fe3O4 Aucomplex cecropin B solution to be detected on the lower slide, performing incubation, flushing the lower slide and drying the lower slide with air; and 5) placing the upper slide on the lower slide,filling a gap between the upper slide and the lower slide with a liquid crystal pool formed by a liquid crystal 5CB, and then observing the color and brightness of a liquid crystal pool optical signal. Through adoption of the method, the detection of the cecropin B can be realized. Moreover, the method has the advantages of low detection cost, high detection sensitivity, relatively small influenceby the objective environment and high detection speed.

Description

technical field [0001] The invention belongs to the technical field of antimicrobial peptide detection, and relates to a 3 o 4 @Au is the method for signal amplifier detection of cecropin B. Background technique [0002] Fe 3 o 4 @Au composite nanoparticles are nanoscale Fe 3 o 4 Composite materials formed with Au, with Fe 3 o 4 Nanoparticles as the core, Au nanoparticles coated in Fe 3 o 4 Formation of Fe around nanoparticles 3 o 4 -Au core-shell structure, also known as gold-magnetic composite particles. It has the advantages of superparamagnetism, good biocompatibility and low toxicity, so it is often used in magnetically targeted drug delivery, bioseparation, sensors, magnetic resonance imaging, antibody labeling and other fields. Cui Yali and others synthesized Fe for the first time in 2001 3 o 4 @Au composite nanoparticles, first synthesize Fe in aqueous solution 3 o 4 nanoparticles, which will be synthesized by Fe 3 o 4 Nanoparticles added as "seeds"...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 苏秀霞刘欢彭正华张婧张海宁徐佳晏春苗胡金龙
Owner SHAANXI UNIV OF SCI & TECH