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Application of cordyceps cicadae ethanol extract in preparation of preparations for treating and/or preventing alteration of intestinal flora and related diseases

A technology of intestinal flora imbalance and alcohol extraction, which is applied to plant/algae/fungi/moss components, metabolic diseases, allergic diseases, etc., can solve the functional scope and market publicity confusion, and the quality control indicators of functional factors are not clear , function is not significant enough and other problems, to achieve the effect of improving physiological and pathological indicators, contributing to the diversification of flora, and obvious therapeutic and/or preventive effects

Inactive Publication Date: 2019-05-21
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the improvement of the health quality of consumers, there is an even greater demand for refined health products. However, most of the existing edible and medicinal fungus health products have a confusing range of functions and market promotion. Functional factors and quality control indicators are not clear, and the functions are not significant enough. Medicinalization, therefore, there is an urgent need for more definite functional factors (active sites), a quality control system with medicinal effects and precision

Method used

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  • Application of cordyceps cicadae ethanol extract in preparation of preparations for treating and/or preventing alteration of intestinal flora and related diseases
  • Application of cordyceps cicadae ethanol extract in preparation of preparations for treating and/or preventing alteration of intestinal flora and related diseases
  • Application of cordyceps cicadae ethanol extract in preparation of preparations for treating and/or preventing alteration of intestinal flora and related diseases

Examples

Experimental program
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Effect test

preparation example Construction

[0051] In the fifth aspect of the present invention, there is provided a method for preparing a cicadae alcohol extract, comprising: after pulverizing the cicadae fruiting body, using 12-16 times the amount of alcohol aqueous solution, ultrasonically extracting for 1-2 hours to obtain an extraction solution, Concentrate under reduced pressure, adjust the pH to 4-5, refrigerate, precipitate, and freeze-dry the precipitate to obtain an alcohol extract.

[0052] Preferably, the aqueous alcohol solution is an aqueous ethanol solution.

[0053] Further preferably, the aqueous ethanol solution is an aqueous ethanol solution with a concentration of 50%-90%.

[0054] Preferably, the cold storage is cold storage for 24 hours.

[0055] Preferably, the pH adjustment is to adjust the pH to 4-5 by adding acetic acid.

[0056] In the sixth aspect of the present invention, there is provided a cicadacil alcohol extract prepared by the above preparation method.

[0057] Cicada (Isaria cicad...

Embodiment 1

[0062] Embodiment 1: the preparation method of cicadaline alcohol extract, the steps are as follows:

[0063] After crushing the cicadae fruiting body, use 12-16 times the amount of ethanol aqueous solution, ultrasonically extract for 1-2 hours to obtain the extraction solution, concentrate it under reduced pressure to an appropriate volume, add acetic acid to adjust the pH to 4-5, refrigerate for 24 hours, and precipitate , take the precipitate and freeze-dry to obtain the cicadaline alcohol extract.

[0064] Protein content was determined by the Kjeldahl method.

[0065] Table 1 is the protein content in the cicadaline alcohol extract.

[0066] Protein content in table 1 cicadae alcohol extract

[0067]

Embodiment 2

[0068] Example 2: The improvement effect of cicadae alcohol extract on the indicators related to intestinal flora imbalance in mice induced by high-fat diet

[0069] 1. Reagents:

[0070] Total cholesterol (T-CHO) test kit: Nanjing Jiancheng Institute of Bioengineering.

[0071] Triglyceride (TG) test kit: Nanjing Jiancheng Institute of Bioengineering.

[0072] Low-density lipoprotein cholesterol (LDL-C) test kit: Nanjing Jiancheng Bioengineering Institute.

[0073] High-density lipoprotein cholesterol (HDL-C) test kit: Nanjing Jiancheng Bioengineering Institute.

[0074] Thirty female C57 mice, SPF grade, weighing 12g-14g, aged 28-30 days, were purchased from Guangdong Medical Experimental Animal Center, license number SCXK (Guangdong) 2013-0002.

[0075] Rats and mice maintained feed: Guangdong Medical Experimental Animal Center, license number SCXK (Guangdong) 2013-0002.

[0076] The high-fat and high-cholesterol feed formula is shown in Table 2.

[0077] Table 2 Main ...

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Abstract

The invention relates to application of an edible fungus extract in preparation of preparations for treating and / or preventing alteration of intestinal flora and related diseases, in particular to application of a cordyceps cicadae ethanol extract in preparation of preparations for treating and / or preventing alteration of intestinal flora and related diseases. The preparation method is simple andpracticable, the operation cost is low, only ethanol and water are used as the extraction solvents in the preparation method, the ethanol is recyclable, and no organic reagent residue risk exists. Thecordyceps cicadae ethanol extract is not only remarkable for helping recovering of the dominant bacterial community structure in the intestinal tract and diversifying the bacterial community but alsohas the remarkable curing and / or preventing effect to the diseases related to the dominant bacterial community structure, and the edible fungus extract can be used in various fields of drugs, healthcare products and the like and has great economic and social values.

Description

technical field [0001] The invention relates to the use of an edible fungus extract in the preparation of preparations for treating and / or preventing intestinal flora disorders and related diseases, in particular to the use of cicadae alcohol extracts in the preparation of treating and / or preventing intestinal flora disorders The use in the preparation of disease and related diseases. Background technique [0002] In recent years, studies on gut microbiota have been emerging, and the role of gut microbiota in human health has attracted more and more attention. Gut flora is the sum of all microorganisms in the human intestinal tract. There are many types (including bacteria, fungi and viruses), and the number is even more amazing. It is estimated that in a normal adult, the total weight of intestinal bacteria can reach 1-1.5kg. They can affect body weight, digestion and absorption capacity, and resist the risk of infection and autoimmune diseases; in addition, they are also...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/068A61P1/00A61P37/04A61P3/06A61P3/04A61P31/04A61P29/00A61P37/08A61P25/00A61P35/00A23L33/10A23L31/00A61K35/64
Inventor 陈地灵唐晓萃郭殷锐吴清平谢意珍帅欧杨柏华
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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