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A kind of preparation method of olaquindox molecularly imprinted membrane electrochemical sensor

A molecularly imprinted membrane, electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of high detection environment requirements, unstable enzymes and antibodies, time-consuming sample pretreatment, etc., to overcome the processing process The effect of cumbersome, low detection limit, convenient adsorption and elution

Inactive Publication Date: 2021-01-08
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantages of instrumental analysis are high accuracy and precision, but often require expensive and complicated instruments, and sample pretreatment is time-consuming
Analytical methods based on enzyme and antigen-antibody reactions have the advantages of high specificity and selectivity, but due to the instability of enzymes and antibodies, the requirements for the detection environment are very high

Method used

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  • A kind of preparation method of olaquindox molecularly imprinted membrane electrochemical sensor
  • A kind of preparation method of olaquindox molecularly imprinted membrane electrochemical sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Preparation of polydopamine@graphene composite

[0043]Dopamine hydrochloride was made into 1 mg / mL with Tris-HCl buffer solution (pH=8.5), and then the graphene powder was dispersed into 1 mg / mL with the above-mentioned solution, protected from light, stirred by magnetic force for 5 h, and then washed with double distilled water , filtered several times, dried in a vacuum oven, and set aside.

[0044] (2) Electrode pretreatment

[0045] The surface of the glassy carbon electrode was polished with 1.0 μm, 0.3 μm, and 0.05 μm alumina powder in sequence, and after being completely rinsed with double distilled water, the 3 1mmol / L K 3 [Fe(CN) 6 ] The solution was scanned by cyclic voltammetry in the range of -0.2 to 0.6V for multiple cycles until a stable electrochemical response was obtained, and then dried in air for later use.

[0046] (3) Drop-coating polydopamine@graphene composite

[0047] Prepare the synthesized polydopamine@graphene polymer with N, N-dimet...

Embodiment 2

[0055] (1) Preparation of polydopamine@graphene composite

[0056] Dopamine hydrochloride was made into 1 mg / mL with Tris-HCl buffer solution (pH=8.5), and then the graphene powder was dispersed into 1 mg / mL with the above-mentioned solution, protected from light, stirred by magnetic force for 5 h, and then washed with double distilled water , filtered several times, dried in a vacuum oven, and set aside.

[0057] (2) Electrode pretreatment

[0058] The surface of the glassy carbon electrode was polished with 1.0 μm, 0.3 μm, and 0.05 μm alumina powder in sequence, and after being completely rinsed with double distilled water, the 3 1mmol / L K 3 [Fe(CN) 6 ] The solution was scanned by cyclic voltammetry in the range of -0.2 to 0.6V for multiple cycles until a stable electrochemical response was obtained, and then dried in air for later use.

[0059] Such as figure 1 As shown, cyclic voltammetry is an effective method to monitor the electron transfer process on the modified ...

Embodiment 3

[0069] (1) Preparation of polydopamine@graphene composite

[0070] Dopamine hydrochloride was made into 1 mg / mL with Tris-HCl buffer solution (pH=8.5), and then the graphene powder was dispersed into 1 mg / mL with the above-mentioned solution, protected from light, stirred by magnetic force for 5 h, and then washed with double distilled water , filtered several times, dried in a vacuum oven, and set aside.

[0071] (2) Electrode pretreatment

[0072] The surface of the glassy carbon electrode was polished with 1.0 μm, 0.3 μm, and 0.05 μm alumina powder in sequence, and after being completely rinsed with double distilled water, the 3 1mmol / L K 3 [Fe(CN) 6 ] The solution was scanned by cyclic voltammetry in the range of -0.2 to 0.6V for multiple cycles until a stable electrochemical response was obtained, and then dried in air for later use.

[0073] (3) Drop-coating polydopamine@graphene composite

[0074] Prepare the synthesized polydopamine@graphene polymer with N, N-dime...

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Abstract

The invention provides a making method for an olaquindox molecularly imprinted membrane electrochemical sensor. The surface of a glassy carbon electrode is coated with a composite membrane, and the composite membrane is composed of a polydopamine-coating graphene complex, polypyrrole and a molecularly imprinted polymer. The making method provided in the invention has the advantages that two properties of dopamine are skillfully used: the first property is adhesion and dispersion, the polydopamine-coating graphene composite membrane has the adhesion of dopamine, overcomes the shortcoming of easy falling of the traditional dispensing method and also has a large specific surface area of graphene, and the second property is a functional monomer, dopamine is polymerized to form a molecularly imprinted membrane, and a molecularly imprinted hole corresponding to an olaquindox molecule is provided. Pyrrole polymerization can increase the conductivity but also further enhance the stability of the polydopamine-coating graphene layer. The shortcomings of complex pretreatment and expensive instrument of a large instrument used in the traditional olaquindox detection method and time consumptionof the traditional synthetic molecularly imprinted method can be overcome.

Description

technical field [0001] The invention belongs to the technical field of molecular detection, and in particular relates to a preparation method of an electrochemical sensor of olaquindox molecularly imprinted membrane. Background technique [0002] Olaquindox belongs to quinoxaline drugs, has good broad-spectrum antibacterial effect, can improve feed digestibility and promote growth of livestock and poultry, and is widely used in veterinary drugs and feed additives. In the feed processing industry, it is often formulated as a feed premix with starch or wheat bran, calcium hydrogen phosphate, and calcium carbonate. Studies have shown that olaquindox has a certain accumulation of toxicity, enters the human body through the food chain and enriches, affects the metabolic balance of the body, has mutagenic, teratogenic, carcinogenic and other hazards, and indirectly threatens human health. Lalaquindox is all prohibited to be used as feed additive in the U.S. and the European Union...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/416G01N27/48
Inventor 方国臻白晓云张博赵鹏王硕
Owner TIANJIN UNIV OF SCI & TECH
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