Method for preparing high-purity xylooligosaccharide

A xylo-oligosaccharide and high-purity technology, which is applied in the preparation of oligosaccharides and sugar derivatives, chemical instruments and methods, etc., can solve problems such as waste of resources, and achieve the effects of full utilization, good economic benefits and application prospects.

Active Publication Date: 2019-05-24
GUANGZHOU YINNOVATOR BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the focus of the research is only to recover the lignin in the cooking black liquor, and there is no effective recovery and purification method for the hemicellulose separated from the plant raw materials in the cooking black liquor
[0006] In addition to the pulping industry, such problems also exist in industries such as biorefinery. Different processing methods are used to process plant raw materials, and the plant extract is obtained after extracting cellulose. If it cannot be used efficiently, it will cause resource pollution. waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Use activated carbon to carry out adsorption treatment on the solution after removing lignin from the plant extract, control the quality of activated carbon to be 3% of the solution, filter after the treatment, and obtain activated carbon and purified liquid I, wherein the activated carbon can be regenerated by heating reuse;

[0040] (2) Wash the strongly acidic cation exchange resin until it is neutral and then pack it into a column, pass through the purified liquid I obtained in step (1) to absorb the monosaccharide in it, and then control the temperature at 80°C and the flow rate at 2.5mL / min for washing off to obtain the purified solution II;

[0041] (3) Mix the purified solution II obtained after absorbing monosaccharides in step (2) evenly with diethyl ether, at 50° C., at a speed of 100 r / min, use diethyl ether to extract the impurities therein, and use three-stage extraction to control the organic solvent and The mass ratio of the purified solution II is ...

Embodiment 2

[0044] (1) Use activated carbon to adsorb the solution after removing lignin from the plant extract, control the quality of activated carbon to be 0.5% of the solution, and filter after the treatment to obtain activated carbon and purified liquid I, wherein the activated carbon can be regenerated by heating reuse;

[0045] (2) Wash the strongly acidic cation exchange resin until it is neutral, then load it into a column, pass through the purified liquid I obtained in step (1), absorb the monosaccharide in it, and then control the temperature at 60°C and the flow rate at 5mL / min for elution , to obtain the purified solution II;

[0046] (3) Mix the purified liquid II obtained after absorbing monosaccharides in step (2) evenly with ethanol, and at 40°C and at a speed of 120r / min, use ethanol to extract the impurities therein, and use three-stage extraction to control the organic solvent and The mass ratio of the purified liquid II is 2:1, the extract phase is evaporated in a va...

Embodiment 3

[0049] (1) Use activated carbon to adsorb the solution after removing lignin from the plant extract, control the quality of activated carbon to be 5% of the solution, filter after the treatment, and obtain activated carbon and purified liquid I, wherein the activated carbon can be regenerated by heating reuse;

[0050] (2) Wash the strongly acidic cation exchange resin until it is neutral and then pack it into a column, pass through the purified solution I obtained in step (1) to absorb the monosaccharide in it, and then control the temperature at 50°C and the flow rate at 4mL / min for elution , to obtain the purified solution II;

[0051] (3) Mix the purified liquid II obtained after absorbing monosaccharides in step (2) evenly with ethanol, and at 40°C and at a speed of 150r / min, use ethanol to extract the impurities therein, and use three-stage extraction to control the organic solvent and The mass ratio of the purified solution II is 3:1, and the extract phase is evaporate...

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PUM

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Abstract

The invention relates to a method for preparing high-purity xylooligosaccharide. The method includes subjecting a solution, obtained by removing lignin from black liquor or plant extract, to adsorption treatment by activated carbon, and performing solid-liquid separation to obtain purified liquid I; adsorbing monosaccharide in the purification liquid I by strong acidic cation exchange resin; mixing purified liquid II obtained after absorption with an organic solvent, removing impurities through purification and extraction, and concentrating and crystallizing a xylooligosaccharide solution obtained after impurity removal to obtain the high-purity xylooligosaccharide. The method has the advantages that the solution obtained by removing the lignin from the black liquor or the plant extract isused as a raw material, and is sequentially subjected to the steps of decolorization and adsorption by the activated carbon, adsorption by the strong acid cation exchange resin, purification and extraction by the organic solvent, and the like to obtain the high-purity xylooligosaccharide of which the purity is higher than 95%; resources are fully utilized, and the method has good economic benefits and application prospect.

Description

technical field [0001] The invention relates to the field of plant separation and recovery, in particular to a method for preparing high-purity xylooligosaccharides. Background technique [0002] Xylooligosaccharides, also known as xylooligosaccharides, are functional polymeric sugars formed by combining 2-7 xylose molecules with β-1,4 glycosidic bonds. Compared with other oligosaccharides, xylooligosaccharides have very good stability and are difficult to be decomposed by human digestive enzymes. Xylo-oligosaccharides can selectively promote the proliferation of intestinal bifidobacteria, and its bifidofactor function is 10-20 times that of other polymeric sugars, because it can reduce the production of toxic fermentation products and harmful bacterial enzymes, and inhibit pathogenic bacteria And diarrhea, prevent constipation, protect liver function, reduce serum cholesterol, lower blood pressure, enhance the body's immunity and anti-cancer and other effects, it has been ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/06C07H3/04C07H1/08C08B37/00
Inventor 张睿哲闵渝刘运思甘梓峰
Owner GUANGZHOU YINNOVATOR BIOTECH CO LTD
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