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Bacillus liquid fermented cultivation method

A technology of fermentation culture and bacillus, applied in the field of microbial fermentation, can solve the problems of complex production technology, high product price, high temperature, etc., and achieve the effect of overcoming high cost, high substrate utilization rate, and improved sensory characteristics

Inactive Publication Date: 2019-06-14
广西康佳龙农牧集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production technology is complicated and the requirements are high, resulting in higher product prices. At the same time, the feed processing and granulation process is at a higher temperature. Common lactic acid bacteria cannot tolerate it, but Bacillus coagulans is a special lactic acid bacterium that can overcome this defect.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A bacillus liquid fermentation culture method, comprises the steps:

[0030] (1) Bacteria slant culture: inoculate Bacillus coagulans and Bacillus subtilis on the slant medium, and culture at 25°C for 20 hours to obtain activated mixed strains; the mass ratio of Bacillus coagulans to Bacillus subtilis is 2:1 Slant medium, by mass fraction: beef extract 2%, glucose 1%, sodium acetate 0.1%, peptone 0.1%, agar 0.2%, dipotassium hydrogen phosphate 0.2%, and the balance is water;

[0031] (2) Seed liquid preparation: inoculate the activated mixed strains in the seed liquid culture medium and cultivate to obtain the seed liquid; seed liquid culture medium, by mass fraction: brown sugar 2%, glucose 1%, sodium acetate 0.1%, urea 0.1%, dipotassium hydrogen phosphate 0.2%, manganese sulfate 0.001%, magnesium sulfate 0.01%, the balance is water, pH7.0-7.2; after the seed liquid culture medium is prepared, it is heated and dissolved, and then lowered to below 40 °C and packed in I...

Embodiment 2

[0035] A bacillus liquid fermentation culture method, comprises the steps:

[0036] (1) Bacteria slant culture: inoculate Bacillus coagulans and Bacillus subtilis on the slant medium, and culture at 30°C for 10 hours to obtain activated mixed strains; the mass ratio of Bacillus coagulans to Bacillus subtilis is 1:3 Slant medium, by mass fraction: beef extract 1%, glucose 0.5%, sodium acetate 0.05%, peptone 0.05%, agar 0.1%, dipotassium hydrogen phosphate 0.1%, and the balance is water;

[0037](2) Seed liquid preparation: inoculate the activated mixed strains in the seed liquid culture medium and cultivate to obtain the seed liquid; seed liquid culture medium, by mass fraction: brown sugar 1%, glucose 0.5%, sodium acetate 0.05%, urea 0.05%, dipotassium hydrogen phosphate 0.1%, manganese sulfate 0.005%, magnesium sulfate 0.05%, the balance is water, pH7.0; the seed liquid culture medium is prepared and heated to dissolve, and then lowered to below 40 ℃ and distributed in 500mL ...

Embodiment 3

[0041] A bacillus liquid fermentation culture method, comprises the steps:

[0042] (1) Bacteria slant culture: inoculate Bacillus coagulans and Bacillus subtilis on the slant medium, and culture at 30°C for 24 hours to obtain activated mixed strains; the mass ratio of Bacillus coagulans to Bacillus subtilis is 5:3 Slant medium, by mass fraction: beef extract 3%, glucose 4%, sodium acetate 0.2%, peptone 0.2%, agar 0.4%, dipotassium hydrogen phosphate 0.4%, and the balance is water;

[0043] (2) Seed liquid preparation: inoculate the activated mixed strains in the seed liquid culture medium and cultivate to obtain the seed liquid; seed liquid culture medium, by mass fraction: brown sugar 3%, glucose 2%, sodium acetate 0.3%, urea 0.3%, dipotassium hydrogen phosphate 0.4%, manganese sulfate 0.001%, magnesium sulfate 0.01%, the balance is water, pH7.0; after the seed liquid medium is prepared, heat and dissolve, and then lower it to below 40 °C and distribute it in 500mL triangula...

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PUM

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Abstract

The invention discloses a bacillus liquid fermented cultivation method, which comprises the slant surface cultivation of the strain, preparation of the seed liquid and the fermentation culture. A seedliquid medium comprises the following components according to the mass fraction: 1-3% OF brown sugar, 0.5-2% of glucose, 0.05-0.3% of sodium acetate, 0.05-0.3% of urea, 0.1-0.4% of dipotassium hydrogen phosphate, 0.001-0.005% of manganese sulfate, 0.01-0.05% of magnesium sulfate, and the balance of water, wherein a pH value is 7.0-7.2. The method adopts the fermented cultivation of the mixed bacteria of Bacillus coagulans and Bacillus subtilis, mutualism and synergism between the strains are generated by a liquid fermentation method. The prepared bacillus liquid has high live bacteria content, good stability and long shelf life, and is especially suitable for feed production and breeding.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for fermenting and cultivating bacillus liquid. Background technique [0002] Bacillus coagulans is a facultative anaerobic bacterium that can grow in both aerobic and anaerobic environments, and the survival rate reaches 96.4% at 100°C for 10 minutes. It can adapt to the low-oxygen intestinal environment, has high tolerance to acid and bile, can carry out lactic acid fermentation, and the L-lactic acid produced can reduce the pH value of the intestinal tract, inhibit harmful bacteria, and promote beneficial bacteria such as bifidobacteria. Bacterial growth and reproduction. Bacillus coagulans can form spores, which is beneficial to restore the microecological balance of the gastrointestinal tract compared with other non-lactic acid producing bacillus. Bacillus coagulans spores can germinate in the human body in about 4-6 hours, and 85% of the bacteria c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/07C12R1/125
Inventor 苏善高赵文青古士德韦爱芬
Owner 广西康佳龙农牧集团有限公司