Chemiluminiscence detection kit for B-type natriuretic peptide and preparation method of chemiluminiscence detection kit

Abstract

The invention discloses a chemiluminiscence detection kit for a B-type natriuretic peptide precursor and a preparation method of the chemiluminiscence detection kit. The kit comprises magnetic particles coupled with a B-type natriuretic peptide precursor capture antibody, an acridinium ester labeled B-type natriuretic peptide precursor detection antibody, a B-type natriuretic peptide precursor system column standard substance, a chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution. The kit provided by the invention takes the magnetic separation chemiluminescence technology as a detection means, and is combined with the acridinium ester labeling technology at the same time. Compared with the existing technology for detecting the B-type natriuretic peptide precursor, the kit has the advantages of high sensitivity, high signal-to-noise ratio and rapid detection.

Description

technical field [0001] The invention belongs to the technical field of immunodetection and analysis, in particular to a B-type natriuretic peptide precursor chemiluminescence immunoassay kit and a preparation method thereof. Background technique [0002] BNP is one of a family of structurally identical natriuretic peptides, the natriuretic peptide system including ANP, BNP and CNP. ANP and BNP are products secreted by the atrium and ventricle. Most of the BNP is mainly secreted by the left ventricle. It is secreted from the precursor protein (proBNP). ProBNP is cleaved into active BNP and inactive NT-proBNP. The secretion of BNP can produce natriuretic, diuretic, vasodilation and smooth muscle relaxation effects. ANP has the same function as BNP, but is mainly secreted by the atrium. Plasma ANP and BNP can lead to elevation in various pathological conditions, especially in the condition of increased wall tension, increased circulating volume (such as: heart failure, renal ...

AI Technical Summary

Problems solved by technology

Among them, although radioimmunoassay and radioimmunoassay have the advantages of high sensitivity and high specificity, there are many operating steps, special testing equipment is required, reagents are expensive, matching instruments are required and there is radioactive contamination, which makes the operation difficult. People are exposed to radiation hazards; in addition, radioactive isotopes are easy to decay, have a short validity period, and are not easy to store

The ELISA method uses horseradish peroxidase or alkaline phosphatase markers. The enzyme markers are easily inactivated, and the chromogenic substrate is easily decomposed when exposed to light. The sensitivity is low, and it has a certain cross-reactivity to compounds with similar structures. inaccurate test results

[0005] CN 102323418 A (2016.02) discloses a kit for detecting B-type natriuretic peptide precursor by chemiluminescence. The kit uses alkaline phosphatase to label the B-type natriuretic peptide precursor monoclonal antibody. The disadvantage of using alkaline phosphatase The main reason is that enzymatic catalysis is greatly affected by the environment: such as disinfectant, pH, and ions will affect it and affect the measurement results, and the time for the substrate to reach the plateau is long, and the cost of the substrate is high, resulting in high detection costs.