Application of atropa-belladonna ornithine decarboxylase to cultivating high-yield hyoscyamine and anisodamine atropa-belladonna varieties

A technology of belladonna ornithine decarboxylase and nightshade ornithine decarboxylase, applied in the field of application of belladonna ornithine decarboxylase in cultivating high-yielding hyoscyamine and anisodamine belladonna varieties, can solve the problem of undeveloped enzymes Kinetic experiments, research needs to be in-depth and other issues, to achieve the effect of increasing TAs content and deepening understanding
CN110004175APending Publication Date: 2019-07-12SOUTHWEST UNIV
1 Cites 1 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTHWEST UNIV
Publication Date
2019-07-12

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses application of atropa-belladonna ornithine decarboxylase to cultivating high-yield hyoscyamine and anisodamine atropa-belladonna varieties. The amino acid sequence coded by theatropa-belladonna ornithine decarboxylase is shown as SEQ ID NO.12, and as the ornithine decarboxylase is over expressed in atropa belladonna, rooting of the atropa belladonna can be improved, and the content of putrescine, N-methyl putrescine and TAs in plants can be increased; by researching AbODC, functions of the AbODC in polyamine and tropane-alkaloid biosynthesis are definite, understandingof biosynthetic pathways of tropane alkaloids can be deepened, and the AbODC has important theoretical value; related results of researching also indicate that the AbODC has the important applicationvalue in the aspects of improving the TAs biosynthetic pathways with a metabolic engineering technology and increasing the TAs content.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to the application of belladonna ornithine decarboxylase in cultivating high-yielding hyoscyamine and anisodamine belladonna varieties. Background technique

[0002] Tropine alkaloids are a class of natural alkaline organic compounds with significant physiological activity derived from a small number of Solanaceae plants. They are widely used clinically as anticholinergic drugs for anesthesia, analgesia, cough relief, hypnosis, anti-motion sickness and treatment Parkinson et al. Hyoscyamine, anisodamine, and scopolamine are commonly used clinically. These TAs mainly rely on the extraction from a few TAs medicinal plants of Solanaceae, but the content of TAs in wild tropane-producing plants is generally low, and the content of scopolamine and scopolamine is extremely low, resulting in high commercial production costs of TAs. Therefore, cultivating high-yield plant materials of TAs is t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More