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Method for detecting palladium ion based on fluorescent carbon quantum dot

A technology of carbon quantum dots and green fluorescence, applied in fluorescence/phosphorescence, measuring devices, and material analysis through optical means, can solve the problems of high price and complicated operation, and achieve convenient operation, good water solubility, and short reaction time Effect

Inactive Publication Date: 2019-07-16
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional detection methods for palladium ions include atomic absorption method, inductively coupled plasma mass spectrometry and electrochemical method. These methods have the characteristics of good selectivity and high sensitivity, but the required instruments are expensive and complicated to operate. limit its application

Method used

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  • Method for detecting palladium ion based on fluorescent carbon quantum dot
  • Method for detecting palladium ion based on fluorescent carbon quantum dot
  • Method for detecting palladium ion based on fluorescent carbon quantum dot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Preparation of green fluorescent carbon quantum dot materials (λ ex =400nm)

[0029] (1) Measure 40mL of distilled water and 7.2mL of ethylenediamine, mix them, then add 1mmol of DTNB, namely 5,5'-dithiobis(2-nitrobenzoic acid), and sonicate at room temperature Time 20min, ultrasonic power 100W, the solution turns from colorless to deep red;

[0030] (II) Put the above solution into the autoclave for carbonization, the temperature is set to 200°C, the pressure is 1.5MPa, the stirring speed is 370r / min, and the reaction time is 18h;

[0031] (III) Take out the solution obtained by carbonization, first filter it through a 0.22 μm canned needle filter, use a rotary evaporator to concentrate the filtrate to about 3-5 mL, and then pass it through a gel column (Shanghai Yuanye Biological Technology Co., Ltd. Technology Co., Ltd., Dextrose G-25 / Sephadex G-25) purification. The gel needs to be soaked in distilled water before use, and the upper layer of foam should be ski...

Embodiment 2

[0034] 1) use Na 2 HPO 4 12H 2 O and NaH 2 PO 4 2H 2 O and deionized water to prepare a PBS buffer solution with a pH of 1.0 to 14.0;

[0035] 2) Weigh 0.0400g of the solid green fluorescent carbon quantum dot material prepared in Example 1, dissolve it in 50.0mL of deionized water, and make a 0.8mg / mL solution;

[0036] 3) Pipette 0.2mL of the 0.8mg / mL solution, and then add the solution with a pH of 1 to 14 to 4.0mL, stir rapidly for 10s, transfer it into a cuvette, and record its fluorescence intensity with a fluorometer to obtain figure 2 (a).

[0037]4) Experiments found that when the pH is 7 or 8, the fluorescence intensity is greater. Then prepare PBS buffer solutions with pHs of 7.2, 7.4, 7.6, and 7.8, repeat the above operations, and measure the fluorescence intensity. It is found that when the pH=7.4, the fluorescence intensity of the solution is the highest.

[0038] 5) figure 2 (b) shows that the fluorescence of green fluorescent carbon quantum dots show...

Embodiment 3

[0040] 1) Prepare 0.8 mg / mL green fluorescent carbon quantum dot material solution. Weigh 0.0400 g of the solid green fluorescent carbon quantum dot material prepared in Example 1, dissolve it in 50.0 mL of PBS buffer solution with a pH of 7.4, and prepare a 0.8 mg / mL solution (as a mother solution).

[0041] 2) Take 0.2mL of the mother solution, dilute it to 4mL with PBS buffer solution with pH=7.4, stir rapidly for 10s, transfer it into a 4mL cuvette, put it into a fluorometer, record the fluorescence intensity at this time, and take it as 0min. Afterwards, record the fluorescence value every 10 minutes until 80 minutes, and get image 3 (a);

[0042] 3) Prepare 0.01M PdCl 2 solution. Take 0.2mL of the mother solution, dilute it to 4mL with PBS buffer solution with pH=7.4, stir rapidly for 10s, transfer it into a 4mL cuvette, and put it into a fluorometer to record the fluorescence intensity at this time. Then take 0.2mL of mother liquor, add 5, 10, 15, 20, 25, 30, 35, 4...

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Abstract

The invention provides a method for detecting a palladium ion based on a fluorescent carbon quantum dot. The method for detecting the palladium ion based on the fluorescent carbon quantum dot is characterized by the steps that step 1, distilled water and ethidene diamine with the volume ratio of 5 to (1-6) to 1 are mixed, 1mmoL DTNB (5,5'-double disulfide generation (2-nitrobenzoic acid)) is added, and ultrasonic processing is carried out for 10-15 min under the indoor temperature condition; step 2, a solution obtained in the step 1 is added into a high-pressure reaction kettle for carbonization, the temperature is arranged as 160-210 DEG C, the stirring speed is 350-400r / min, and the reaction time is 12-24h; and the step 3, a solution obtained in the step 2 is taken out, filtration and concentration are carried out, then gel column purification and filter liquor collection are carried out, concentration is carried out again, and drying is carried out to obtain a green fluorescent carbon quantum dot material. The method for detecting the palladium ion based on the fluorescent carbon quantum dot is simple and convenient to operate, an obtained probe can rapidly detect a Pd2+ion, andgood selectivity, sensitivity and reproducibility are achieved on the palladium ion. Meanwhile, it is found that other metal ions, heavy metal ions and amino acid substances have tiny interference onthe detection system.

Description

technical field [0001] The invention relates to a method for detecting palladium ions based on fluorescent carbon quantum dots, and belongs to the technical field of nanomaterial preparation and application. Background technique [0002] It is well known that heavy metals play an important role in scientific fields such as chemistry, environment and physics. Among them, palladium is a precious metal. Due to its special properties, precious metal palladium has been widely used in various fields. However, the extensive use of palladium also brings great harm to the environment, which has aroused widespread concern. For example, the discharge of loaded palladium and its compounds will not only greatly increase the palladium content in different environments such as soil and water, but also accumulate in the liver, spleen, kidney and other organs through the enrichment effect in the body, causing serious damage to the body. The researchers found that palladium ions (Pd 2+ ) ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/643G01N2021/6417
Inventor 徐虎李明中王宇红
Owner SHANGHAI INST OF TECH
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