Novel pyrenyl polysiloxane and application thereof
A pyrene-based polysiloxane, a new type of technology, applied in instruments, analytical materials, measuring devices, etc., can solve the problems of low responsiveness and sensitivity, damage to the liver, death, etc., and achieve high sensitivity, low cost, and broad application prospects. Effect
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Embodiment 1
[0035] The preparation of pyrenyl polysiloxane PySQ-4 of the present invention
[0036] The preparation method of pyrenylpolysiloxane PySQ-4 of the present invention is: take respectively vinyl silicone oil (1g), 1-bromopyrene (0.28g, 1mmol), palladium acetate (15mg) and Tri(o-methylphenyl)phosphorus (30mg) was dissolved in a 100mL flask by adding 5mL triethylamine and 30mL toluene, then heated to 90°C under N 2 Reflux under protection for 48h to fully react. After the reaction, cool to room temperature, filter, and evaporate the filtered solution under reduced pressure. The precipitate obtained after washing with methanol and tetrahydrofuran is the target novel pyrenylpolysiloxane PySQ-4. The above-mentioned pyrenylpolysiloxane 1 H NMR spectrum see figure 1 .
Embodiment 2
[0038] Novel pyrenyl polysiloxane PySQ-4 of the present invention is to the selective response fluorescence curve (fluorescence chromatographic analyzer analysis) of NT
[0039] Prepare a PySQ-4 solution with a concentration of 0.02 mg / mL and a solvent of dichloromethane, and add the same volume of nitrotoluene derivative solutions (trinitrotoluene (TNT), dinitrotoluene (DNT), 4-nitrotoluene nitrotoluene (NT)), the fluorescence quenching curve of PySQ-4 solution p-nitrotoluene derivatives was tested by fluorescence chromatography. For the fluorescence spectrum obtained above, see figure 2 . After adding NT, the fluorescence of the solution was almost completely quenched, while the fluorescence intensity did not change significantly after adding the other two nitrotoluene derivatives, indicating that PySQ-4 has good selectivity for NT.
Embodiment 3
[0041] Novel pyrenyl polysiloxane PySQ-4 of the present invention is to the concentration response fluorescence curve (fluorescence chromatographic analyzer analysis) of NT to prepare concentration and be 0.02mg / mL, solvent is the PySQ-4 solution of dichloromethane, then constantly drip Add NT solution (0→10ng), and test the fluorescence quenching curve of PySQ-4 solution on NT by fluorescence chromatography. For the fluorescence spectrum obtained above, see image 3 . With the increase of the concentration of NT in the solution, the fluorescence intensity of the solution decreased rapidly until it was completely quenched. The obvious fluorescence intensity can be seen when the NT concentration is 0.1 μg / mL, indicating that PySQ-4 has good responsiveness and high sensitivity to NT.
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