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plga/fk506 drug-loaded nano-microspheres and its preparation method and application

A drug-loaded nanometer and microsphere technology, which is applied in bio-nanomaterials in the field of biomedicine, can solve the problems of difficult promotion of targeted therapy technology, poor drug encapsulation rate, and failure to meet the curative effect requirements, so as to improve active targeting performance, Effect of delayed release, good sustained release effect

Active Publication Date: 2021-03-19
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, at present, the degradation performance and drug release rate of many nano-microspheres cannot match the speed of nerve repair, and the poor drug encapsulation efficiency of drug-loaded nano-microspheres cannot meet the curative effect requirements. Difficult to promote clinically

Method used

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  • plga/fk506 drug-loaded nano-microspheres and its preparation method and application
  • plga/fk506 drug-loaded nano-microspheres and its preparation method and application
  • plga/fk506 drug-loaded nano-microspheres and its preparation method and application

Examples

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Effect test

Embodiment 1

[0046] A kind of preparation method of PLGA / FK506 drug-loaded nano microspheres of the present embodiment, the method comprises the following steps:

[0047] (1) Precisely weigh 125mg of Pluronic F127 with an electronic balance and add it to 10mL of deionized water, dissolve evenly to form a PF127 aqueous solution with a concentration of 12.5mg / mL as the water phase;

[0048] (2) Use an electronic balance to accurately weigh 60 mg of PLGA material and 20 mg of FK506 drug in turn, and then add the weighed PLGA and FK506 into 2 mL of chloroform (organic solvent) in turn, and form a uniform mixture after completely dissolving, as an oil Mutually;

[0049] (3) Accurately measure 30mL of the PF127 water phase prepared in step (1) into a round-bottomed flask, and slowly add the oil phase obtained in step (2) to the water at a speed of 5 s / drop at a speed of 1000 rpm in an ice-water bath. Phase vortex center, after the dropwise addition, the dispersion liquid is obtained; then the o...

Embodiment 2

[0055] A kind of preparation method of PLGA / FK506 drug-loaded nano microspheres of the present embodiment, the method comprises the following steps:

[0056] (1) Precisely weigh 100mg of Pluronic F108 with an electronic balance and add it to 10mL of deionized water, dissolve evenly to form a PF108 aqueous solution with a concentration of 10mg / mL as the water phase;

[0057] (2) Use an electronic balance to accurately weigh 55mg of PLGA material and 30mg of FK506 drug in sequence, then add the weighed PLGA and FK506 into 2.5mL of dichloromethane organic solvent in turn, and form a uniform mixture after the dissolution is complete. Mutually;

[0058] (3) Accurately measure 30mL of the PF108 aqueous phase prepared in step (1) into a round bottom flask, and slowly add the oil phase obtained in step (2) to the water at a speed of 6 s / drop at a speed of 1500 rpm in an ice-water bath. The center of the phase vortex, after the dropwise addition, the dispersion liquid was obtained; th...

Embodiment 3

[0061] A kind of preparation method of PLGA / FK506 drug-loaded nano microspheres of the present embodiment, the method comprises the following steps:

[0062] (1) Precisely weigh 150mg of Pluronic F108 with an electronic balance and add it to 10mL of deionized water, dissolve evenly to form a PF127 aqueous solution with a concentration of 15mg / mL as the water phase;

[0063] (2) Use an electronic balance to accurately weigh 50 mg of PLGA material and 10 mg of FK506 drug in turn, and then add the weighed PLGA and FK506 to a mixed organic solvent composed of 1.25 mL of dichloromethane and 1.25 mL of absolute ethanol, dissolve After completion, a uniform mixed liquid is formed as an oil phase;

[0064] (3) Accurately measure 30mL of the PF108 aqueous phase prepared in step (1) into a round bottom flask, and slowly add the oil phase obtained in step (2) to the water at a speed of 5 s / drop at a speed of 800 rpm in an ice-water bath. The center of the phase vortex, after the dropwis...

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Abstract

The invention relates to a PLGA / FK506 drug-loading nano microsphere and a preparation method and an application thereof. The preparation method for the drug-loading nano microsphere comprises the following steps: firstly adding a nonionic surfactant to deionized water and forming a water phase, enabling PLGA and FK506 to be successively dissolved in an organic solvent and forming an oil phase, after that, dropwise slowly adding the oil phase to a vortex center of the water phase, after high-speed stirring and ultrasonic treatment, acquiring O / W-type emulsion; and stirring the acquired O / W-typeemulsion in a room temperature condition under a low speed so that the organic solvent is completely volatilized, acquiring nano microsphere turbid liquid, finally centrifuging, washing, and drying to obtain a target product, wherein a particle size of the product is 100-200 nm preferably. Drug-loading capacity of the prepared PLGA / FK506 drug-loading nano microsphere reaches 17.64%, and encapsulation efficiency reaches 77.08%. Active targeting performance of the drug-loading microsphere is advantageously improved, and drug slow release and stronger effect of inhibiting growth of scar cells are realized.

Description

technical field [0001] The invention relates to the application of a bionano material in the field of biomedicine, in particular to a PLGA / FK506 drug-loaded nano-microsphere with an active targeting scar inhibitory effect and a preparation method and application thereof. Background technique [0002] Scar formation is currently a major problem in wound healing, which occurs after various in vitro traumas, burns and operations. The generation of scar is an important process that cannot be separated in peripheral nerve repair and wound healing. However, it is generally believed that the appearance of scar has an adverse effect on the repair process of peripheral nerve. Obstacles not only greatly reduce the speed and quality of nerve regeneration, but also lead to swelling of nerve stumps and fibrosis. In severe cases, pain and loss of related functions will be caused, which will affect the quality of life. Therefore, how to inhibit scar cells is an urgent problem to be solved...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/34A61K31/436A61P17/02
CPCA61K9/5153A61K31/436A61P17/02
Inventor 李宾斌殷义霞陈皓冰戴红莲王欣宇
Owner WUHAN UNIV OF TECH
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