Porcine deltacoronavirus strain and application thereof

A technology of coronavirus and strains, applied in the field of porcine delta coronavirus strains, can solve the problems of undeveloped effective vaccines or drugs, and achieve the effect of strong toxicity and good immune effect

Active Publication Date: 2019-08-20
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, PDCoV is a new type of pathogen that has just been discovered in recent years, and currently no effective vaccine or drug for this disease has been developed worldwide.

Method used

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  • Porcine deltacoronavirus strain and application thereof
  • Porcine deltacoronavirus strain and application thereof
  • Porcine deltacoronavirus strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] 1 Disease material and cells

[0035] The disease materials were collected from Xinjiang, China, and 12 samples containing PDCoV-positive strains were used for the isolation and identification of PDCoV; LLC-PK-1 cells were cryopreserved by our laboratory. The cells were subcultured in MEM medium containing 10% FBS and 1% double antibody.

[0036] 2 Determination of S genome sequence of PDCoV CH / XJYN03 / 2016 strain

[0037] According to the published PDCoV sequence comparison results, the conserved regions were selected, and the corresponding amplification primers were designed to amplify the S genome sequence of PDCoV. The primers are shown in Table 1. After the CH / XJYN03 / 2016 strain field feces samples and cell culture virus liquid RNA were extracted, RT-PCR reactions were performed using S genome-specific primers. The pCR-XL-2TOPO vector for blunt end connection was selected for subcloning of the PCR fragment, and the correct plasmid identified by enzyme digestion wa...

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Abstract

The invention provides a porcine deltacoronavirus strain and application thereof, and belongs to the technical field of immunoprophylaxis of pigs. The porcine deltacoronavirus strain is deposited in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 17383. The porcine deltacoronavirus strain is highly toxic. When the porcine deltacoronavirus strain is inactivated to prepare a porcine epidemic diarrhea virus, the immune effect is good.

Description

technical field [0001] The invention belongs to the technical field of immune prevention of pigs, and in particular relates to a porcine delta coronavirus strain and application thereof. Background technique [0002] Porcine Deltacoronavirus (Porcine Deltacoronavirus, PDCoV) is a new member of the genus Deltacoronavirus in the Coronaviridae family. It can cause diarrhea, vomiting, and dehydration in sows and piglets, and can be fatal in severe cases. serious economic loss. In 2012, the novel coronavirus was first detected in Hong Kong, China. In early 2014, an outbreak of diarrheal disease in sows and piglets in pig farms in Ohio, USA, was tested positive for PDCoV, and the new porcine coronavirus has also been detected in at least 18 states in the United States. Subsequently, PDCoV was also detected in fecal samples from diarrheal piglets in South Korea and Canada. In China, from 2013 to 2014, after testing 258 fecal samples collected from pig farms in Jiangsu, Guangdong...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/215A61P31/14C12R1/93
CPCA61K39/12A61K2039/5252A61K2039/5254A61K2039/552A61P31/14C12N7/00C12N2770/20021C12N2770/20034
Inventor 刘新生王永录方玉珍周鹏张永光
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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