Application of insect detoxification enzyme protein for degrading organophosphorus pesticide

Abstract

The invention belongs to an application of an insect detoxification enzyme in pesticide degradation, in particular relates to an application of a detoxification enzyme LmGSTpcF1 in locusta migratoriafor degrading malathion pesticide, The detoxification enzyme LmGSTpcF1 in locusta migratoria has amino acid sequence as shown in SEQ ID NO: 2. After incubating LmGSTpcF1 and GSH with malathion under reaction conditions, the malathion is detected by liquid chromatography and the result show that the malathion is completely degraded substantially and no pesticide residues is detected and the degradation efficiency is more than 99%. The detoxification enzyme LmGSTpcF1 in locusta migratoria can be used to remove malathion pesticide residues in agricultural products, water sources, soils, etc.

Description

technical field

[0001] The invention relates to the application of insect detoxification enzymes in pesticide degradation, in particular to the application of insect glutathione-S transferase protein in malathion degradation. Background technique

[0002] In my country, malathion is widely used in pest control due to its high efficiency, low toxicity and broad-spectrum insecticidal properties. Since the target of malathion is acetylcholinesterase (AChE), it can affect the nervous system of mammals and humans, thereby causing harm. Long-term unreasonable excessive use of malathion not only greatly increases its pesticide residues in the environment, but also leads to insecticide resistance in insects, which further leads to the widespread use of pesticides. If things go on like this, malathion pesticide residues in the environment will affect food safety and people's health, and they need to be treated harmlessly.

[0003] How to remove pesticide residues in a green and eff...

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