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Preparation method of carrier of supported AHL-QS degrading enzyme

A technology of AHL-QS and degrading enzymes, applied in chemical instruments and methods, sustainable biological treatment, water/sludge/sewage treatment, etc., can solve problems such as poor stability, easy inactivation, and easy loss, and achieve Large pore volume, easy operation and low cost

Inactive Publication Date: 2019-08-30
TG HILYTE ENVIRONMENTAL TECHNOLOGY (BEIJING) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, when the AHL degrading enzyme is used alone, there will be disadvantages such as poor stability, easy loss, easy inactivation, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] A preparation method for a carrier loaded with AHL-QS signal molecule degrading enzyme, comprising the steps of:

[0026] (1) UiO-66 loaded with AHL-QS signal molecule degrading enzyme: UiO-66 was synthesized by solvothermal method, UiO-66 was sieved into 30-35 mesh, and then ultrasonically activated to load AHL-QS degrading enzyme , the loading method is the adsorption method, the AHL-QS signal molecule degrading enzyme and UiO-66 are mixed in a phosphate buffer at a mass ratio of 0.75:1,

[0027] pH = 6.5-7.5, and then put it into a shaker with a temperature of 30-35°C and a rotation speed of 100-200r / min for 5-6h of adsorption.

[0028] The phosphate buffer is a mixed solution of trisodium phosphate, potassium dihydrogen phosphate, sodium chloride, potassium chloride and ultrapure water in a mass ratio of 0.01:0.06:0.07:0.001:1.

[0029] The AHL-QS signal molecule degrading enzymes are all enzymes including acyltransferase and lactonase that have a degrading effect ...

Embodiment 1

[0033] (1) UiO-66 loaded with AHL-QS signal molecule degrading enzyme: UiO-66 was synthesized by solvothermal method, UiO-66 was sieved into 35 meshes, and then ultrasonically activated to load AHL-QS degrading enzyme, loaded The method is the adsorption method. AHL-QS signal molecule degrading enzyme and UiO-66 are mixed in a phosphate buffer at a mass ratio of 1:1, pH=7.5. Then, the temperature is set to 35°C and the rotation speed is 200r / Adsorption was carried out in a shaker for 6 hours.

[0034] (2) Preparation of AHL-QS signal molecule degrading enzyme carrier: select the density, size and pore size as 800-1000kg / m respectively 3 , 15×15×15mm and 50PPI polyurethane sponge, use small scissors to cut out an appropriate gap in the polyurethane sponge, use a small medicine spoon to place UiO-66 adsorbed with AHL-QS degrading enzyme inside the sponge, and then insert the sponge into In the hollow spherical plastic carrier with a diameter of 10mm, the gap cut out by the pol...

Embodiment 2

[0036] (1) UiO-66 loaded with AHL-QS signal molecule degrading enzyme: UiO-66 was synthesized by solvothermal method, UiO-66 was sieved into 30 meshes, and then ultrasonically activated to load AHL-QS degrading enzyme, loaded The method is the adsorption method. AHL-QS signal molecule degrading enzyme and UiO-66 are mixed in a phosphate buffer at a mass ratio of 0.75:1, pH=7, and then the temperature is set to 30°C and the rotation speed is 150r / Adsorption was carried out in a shaker for 6 hours.

[0037] (2) Preparation of AHL-QS signal molecule degrading enzyme carrier: select the density, size and pore size as 800-1000kg / m respectively 3 , 15×15×15mm and 50PPI polyurethane sponge, use small scissors to cut out an appropriate gap in the polyurethane sponge, use a small medicine spoon to place UiO-66 adsorbed with AHL-QS degrading enzyme inside the sponge, and then insert the sponge into In the hollow spherical plastic carrier with a diameter of 10mm, the gap cut out by the...

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Abstract

The invention discloses a preparation method of a carrier of a supported AHL-QS signal molecule degrading enzyme. The preparation method comprises the following steps: metal organic framework materialUiO-66 fixes an AHL-QS signal molecule degrading enzyme by an adsorption method, and preparing the carrier of the supported AHL-QS signal molecule degrading enzyme by using a sponge as a substrate material, wherein the prepared carrier ensures the enzyme activity to the utmost extent. The method provided by the invention has the following beneficial effects: the composite film can utilize the AHL-QS signal molecule degrading enzyme to inhibit or degrade signal molecules which determine biofilm formation and extracellular polymer secretion, significantly reduce biofilm formation ability, and control film fouling; and meanwhile the carrier has the advantages of mild preparation conditions, simple and convenient operation, low costs and a good anti-biofilm pollution effect, and can be widelyapplied to various sewage treatment.

Description

technical field [0001] The invention belongs to the technical field of waste water treatment, in particular to the technical field of biological treatment waste and sewage, in particular to a preparation method and application of a carrier loaded with AHL-QS degrading enzyme. Background technique [0002] Membrane bioreactor system (MBRs) is an important water recovery technology that has emerged in recent years. It is a combination of adsorption, biodegradation and membrane separation processes. It has the advantages of long SRT, flexible operation, compact device structure, and small sludge production. , high nitrification performance, high biomass diversity, stable effluent water quality, excellent water quality, suitable for repeated use, etc., have been widely used in various water treatment and recovery. Membrane biofouling, that is, the formation of biofilm by microorganisms on the membrane, will lead to a rapid decrease in permeation flux, a decrease in membrane sepa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F3/12C02F3/10
CPCC02F3/108C02F3/1268C02F3/342Y02W10/10
Inventor 王晓张建清张新波温海涛
Owner TG HILYTE ENVIRONMENTAL TECHNOLOGY (BEIJING) CO LTD
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