Method for separating insect proteins, oil and chitin in one step by natural deep-eutectic solvents, and application thereof

Abstract

The present invention belongs to the field of processing and utilization of agricultural products, and discloses a method for separating insect proteins, oil and chitin in one step by natural deep-eutectic solvents, and an application thereof. The method uses insect powder as a raw material, the insect powder is added to natural deep-eutectic solvents, a heat reaction is conducted until a tissue structure of the insect powder is fully dissociated, an upper layer oil component is collected by centrifugation, a lower part is filtered and washed to collect chitin, a solvent part is subjected to acold treatment to precipitate insect proteins, the solvent is recovered, and finally drying is conducted to obtain an initial extract of the insect chitin and proteins. The natural deep-eutectic solvents are used as a multifunctional medium, and the one-step method can realize the separation of the insect proteins, oil and chitin, and can increase added value of insect resources. The method can completely replace traditional multi-step separation processes, and is simple in processes and mild in conditions, the products are liable to separate, the solvent can be recycled, cost and energy consumption are reduced, and the processes have better economy and environmental protection, so that deep utilization of the insect resources is feasible.

Description

technical field [0001] The invention belongs to the field of processing and utilization of agricultural products, in particular to a method for separating insect protein, oil and chitin with a natural deep eutectic solvent in one step and its application. Background technique [0002] Insects, as the largest animal group in nature, are renewable resources that can be developed and utilized by humans. Insects generally contain high-quality proteins, oils and biologically active substances, which provide a rich material basis for their in-depth development and utilization. [0003] Silkworm chrysalis, pollen worms, black soldier flies and fly maggots are representative insects that can be reared artificially on a large scale at present. They are directly used as compound feed materials in production and breeding, but due to oxidative rancidity of oils, the quality of feed is affected, and improper use causes losses. Studies have shown that the separation of the main component...

AI Technical Summary

Problems solved by technology

Using enzymatic deproteinization, although the pollution is small and the conditions are mild, the efficiency of enzymatic deproteinization is low, multiple enzymatic hydrolysis is required, and other auxiliary means are often needed to coordinate deproteinization, which increases the extraction steps and extraction costs.

Another example is CN200910069014.8 which discloses a method for extracting chitin by microorganisms in the whole process. Shrimp and crab shells are subjected to solid fermentation, and the fermentation parameters are controlled (bacteria powder 5% to 30%, temperature 40°C to 70°C, pH 5 to 8.5, Ventilation volume 0.5~1.6L / min), during the fermentation process, it is necessary to pass hot air and produce waste gas, which requires subsequent treatment before it can be discharged, so the increased production process will increase the cost