Method for separating insect proteins, oil and chitin in one step by natural deep-eutectic solvents, and application thereof

A low eutectic solvent, insect protein technology, applied in the field of natural deep eutectic solvent one-step separation of insect protein, oil and chitin, can solve the problem of increasing extraction steps and extraction costs, low efficiency of enzymatic hydrolysis and proteolysis, and increasing production procedures. and other problems, to achieve the effect of reducing cost and energy consumption, wide solubility, and simple process

Pending Publication Date: 2019-08-30
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using enzymatic deproteinization, although the pollution is small and the conditions are mild, the efficiency of enzymatic deproteinization is low, multiple enzymatic hydrolysis is required, and other auxiliary means are often needed to coordinate deproteinization, which increases the extraction steps and extraction costs.
Another example is CN200910069014.8 which discloses a method for extracting chitin by microorganisms in the w

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0027] Example 1

[0028] Weigh choline chloride and citric acid, mix them according to the molar ratio of 1:1, the total mass is 500g, and add them to the round-bottomed flask in turn, place them in a water bath at 80℃ and keep stirring for 0.5h to form a transparent liquid, which is a natural low Eutectic solvent. Weigh 100g of insect powder and add it to the natural eutectic solvent prepared above, react in a water bath at 100°C for 2h, centrifuge to collect the upper layer of grease, weigh it, and filter the reactant with gauze on the lower layer. Wash the retained chitin with ethanol, collect and mix The solvent was placed at 4°C for 2 hours, centrifuged at 10000 rpm for 5 minutes, and the precipitated protein was collected. The mixed solvent was separated by rotary evaporation to separate ethanol and natural eutectic solvent, and then recovered and reused. Finally, the collected chitin and protein are dried and the yields of insect protein, oil and chitin are 90.2%, 95.5% ...

Example Embodiment

[0029] Example 2

[0030] Weigh a certain mass of betaine and n-butyric acid, mix them according to the molar ratio of 1:2, the total mass is 500g, and add them to a round bottom flask in turn, place them in a water bath at 90℃ and keep stirring for 0.5h to form a transparent liquid, which is a natural low Eutectic solvent. Weigh 50g of insect powder and add it to the natural eutectic solvent prepared above, react in a water bath at 120°C for 1 hour, centrifuge at 5000 rpm for 12 minutes, collect the upper layer of grease, weigh it, and filter the reactant with gauze on the lower layer. The chitin is deionized. Wash with water, place the mixed solvent at -20°C for 1 hour, centrifuge, collect the precipitated protein, and remove the water from the mixed solvent by rotary evaporation, and recover and reuse the natural eutectic solvent. Finally, the collected chitin and protein were dried and the yields of insect protein, oil and chitin were 93.2%, 90.5% and 90.4%, respectively. T...

Example Embodiment

[0031] Example 3

[0032] Weigh a certain mass of choline chloride and oxalic acid, mix them in a molar ratio of 1:1, the total mass is 500g, and add them to a round-bottomed flask in turn, place them in a water bath at 80℃ and keep stirring for 0.5h to form a transparent liquid, which is natural Eutectic solvent. Weigh 125 g of insect powder and add it to the natural eutectic solvent prepared above, and react in a water bath at 140°C for 2 hours. The upper layer of oil is collected by centrifugation and weighed. The lower layer is filtered with gauze to filter the reactants, and the retained chitin is washed with methanol, collected and mixed The solvent was placed at -10°C for 4 hours, and the precipitated protein was collected by centrifugation at 12000 rpm for 5 minutes. The mixed solvent was rotary evaporated to separate methanol and natural eutectic solvent, and then recycled. Finally, the collected chitin and protein were dried and the yields of insect protein, oil and ch...

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PUM

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Abstract

The present invention belongs to the field of processing and utilization of agricultural products, and discloses a method for separating insect proteins, oil and chitin in one step by natural deep-eutectic solvents, and an application thereof. The method uses insect powder as a raw material, the insect powder is added to natural deep-eutectic solvents, a heat reaction is conducted until a tissue structure of the insect powder is fully dissociated, an upper layer oil component is collected by centrifugation, a lower part is filtered and washed to collect chitin, a solvent part is subjected to acold treatment to precipitate insect proteins, the solvent is recovered, and finally drying is conducted to obtain an initial extract of the insect chitin and proteins. The natural deep-eutectic solvents are used as a multifunctional medium, and the one-step method can realize the separation of the insect proteins, oil and chitin, and can increase added value of insect resources. The method can completely replace traditional multi-step separation processes, and is simple in processes and mild in conditions, the products are liable to separate, the solvent can be recycled, cost and energy consumption are reduced, and the processes have better economy and environmental protection, so that deep utilization of the insect resources is feasible.

Description

technical field [0001] The invention belongs to the field of processing and utilization of agricultural products, in particular to a method for separating insect protein, oil and chitin with a natural deep eutectic solvent in one step and its application. Background technique [0002] Insects, as the largest animal group in nature, are renewable resources that can be developed and utilized by humans. Insects generally contain high-quality proteins, oils and biologically active substances, which provide a rich material basis for their in-depth development and utilization. [0003] Silkworm chrysalis, pollen worms, black soldier flies and fly maggots are representative insects that can be reared artificially on a large scale at present. They are directly used as compound feed materials in production and breeding, but due to oxidative rancidity of oils, the quality of feed is affected, and improper use causes losses. Studies have shown that the separation of the main component...

Claims

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Application Information

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IPC IPC(8): C07K1/14C11B1/10C08B37/08
CPCC07K1/145C11B1/10C08B37/0003C08B37/003
Inventor 周鹏飞王旭苹邝哲师丘银清黄静叶明强潘木水
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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