Novel artificial blood vessel

Abstract

The invention relates to the field of tissue engineering and regenerative medicine and designs a bilayer artificial blood vessel by constructing high-molecular ordered fibers and bacterial cellulose and modifying with a specific extracellular matrix. An inner layer of the artificial blood vessel herein is made with the ordered fibers based on the action of fiber morphology upon cell behaviors; a binding site is provided for vascular endothelial cells; polar growth of the vascular endothelial cell is induced. An outer layer is made with bacterial cellulose, so that cell penetration is facilitated, and a survival space is preserved for vascular smooth muscle cells. in-vitro cell culture is performed on the ordered fibers and bacterial cellulose based on structural optimization. In simple words, vascular endothelial cells are cultured on the ordered fibers; vascular smooth muscle cells are cultured with the bacterial cellulose. Finally, cell-specific extracellular matrix modification is performed on the ordered fibers and bacterial cellulose by decellularization, and the behaviors of different cells in different cell regeneration phases are regulated.

Description

technical field [0001] Aiming at the fields of tissue engineering and regenerative medicine, the present invention utilizes ordered polymer fibers and bacterial cellulose to prepare artificial blood vessels, and creatively cultures vascular endothelial cells and smooth muscle cells with ordered polymer fibers and bacterial cellulose, to explore specific The role of the extracellular matrix of cells on self-reseeding. The ordered fibers and bacterial cellulose compounded by the extracellular matrix that vascular endothelial cells and smooth muscle cells rely on for the survival of vascular endothelial cells and smooth muscle cells are obtained by decellularization technology, in order to construct different vascular cell microenvironments in vitro, which is conducive to vascular cell penetration and provides different vascular cells. Specifically adhere to the binding site, promote the transformation of vascular cell phenotype, restore the function of vascular cells, and make t...

AI Technical Summary

Problems solved by technology

However, their potential is limited by availability, cost, processing techniques and clinical outcomes

Although many substitutes have mechanical strengths that match or even exceed native vascular tissue, the structure and composition of their matrices are not conducive to proper repopulation of host cells

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