Nano probe for biological micro-area and photothermal therapy and synthesis method and detection method thereof

A technology of nanoprobes and synthesis methods, applied in chemical instruments and methods, nanotechnology, radiotherapy, etc., can solve the problems of low sensitivity and accuracy, poor stability, and real-time performance, so as to improve sensitivity and accuracy, improve Precise targeting, the effect of improving luminous efficiency

Active Publication Date: 2019-09-06
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The current technology has shortcomings such as poor stability, poor real-time performance, and low sensitivity and accuracy, which cannot meet the requirements

Method used

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  • Nano probe for biological micro-area and photothermal therapy and synthesis method and detection method thereof
  • Nano probe for biological micro-area and photothermal therapy and synthesis method and detection method thereof
  • Nano probe for biological micro-area and photothermal therapy and synthesis method and detection method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0031] Embodiment 1: A near-near-infrared up-conversion nanoprobe and its preparation method, which are specifically realized through the following technical solutions:

[0032] Take 3ml of NaOH aqueous solution, 8ml of ethanol and 20ml of oleic acid and mix them well at room temperature until they become clear, then mix the acetates of Y, Yb, Er and Nd elements with a molar ratio of Y, Yb, Er and Nd of 78:20 : 1:1 Add the above clear solution for doping and stir for 30min; then add 10 ml NaF (8 mmol) ethanol solution, hydrothermally react in a hydrothermal kettle at 190°C for 24 h, centrifuge at 9000r / min to obtain upconversion nanoparticles NaYF4 : Yb / Er / Nd.

[0033] Synthesized upconversion nanoparticles NaYF 4 : Yb / Er / Nd diameter is 50 nm (such as figure 1 , it can be seen that the particle size of up-conversion nanoparticles is about 50 nm, and the particle size is uniform); it can be excited by 808 nm laser (200 mW / cm 2 ) emits green visible light at 520 nm and 545 n...

Embodiment approach 2

[0036] Embodiment 2: The application of up-conversion nanoprobes in real-time temperature detection of biological micro-areas is specifically realized through the following technical solutions:

[0037] Take 100 μl of the upconverting nanoprobe (1 mg / ml) prepared in Embodiment 1, put it into a 1 mL disposable petri dish, blow it evenly and incubate with the cell sample to be tested, wash it with PBS three times after 6 h, and put the petri dish Place on the sample stage of a confocal microscope.

[0038] 808 nm near-infrared laser emits laser light (power density 50 mW / cm 2 ), the excitation light is coupled into the confocal microscope to realize the scanning excitation of the up-conversion nanoprobe in the cell sample to be tested, so that the up-conversion nanoprobe generates visible light at 520 nm and 545 nm, and the above-mentioned visible light is adjusted to two wavelengths of 520 nm and 545 nm After being filtered by the band-pass filter, it is received by the photom...

Embodiment approach 3

[0040] Embodiment 3: Photothermal effect of nanoprobes under high-power laser irradiation

[0041] The aqueous solution of nanoparticles (1ml, 1mg / ml) was placed in a microcentrifuge tube, and after ultrasonication, an 808 nm near-infrared laser (power density 1 W / cm 2 ) irradiate the solution from above the centrifuge tube for 1 minute, and the thermal imager records the temperature gradient of the solution in the tube.

[0042] like Figure 4 , is 1 W / cm 2 Temperature rise diagram of 1 mg / ml upconversion nanoparticle solution under 808 nm laser excitation power.

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Abstract

The invention relates to the technical field of non-contact micro-area temperature detection measurement and discloses a nano probe for biological micro-area and photothermal therapy and a synthesis method and a detection method thereof. Upconversion nanoparticles adopt optical rate detection temperature and are excited by 808 nm laser, heat effect interference of water absorption is avoided, 808nm two-photon near-infrared direct excitation and two-excitation process based on dye energy resonance transfer excitation effectively improve the luminescent conversion efficiency and the temperaturemeasurement sensitivity of the nanoparticles. By applying the nano probe for biological micro-area and photothermal therapy and the synthesis method and the detection method, the non-contact measurement of the temperature of a biological cell micro-area or regional temperature can be achieved, the redundant energy during the strong laser excitation can be converted into heat by adjusting the excitation light power and utilizing the strong absorption of a dye, and the accurate thermal shock and simultaneous temperature monitoring for a tumor photothermal therapy can be achieved.

Description

technical field [0001] The invention relates to the technical field of non-contact micro-area temperature detection and measurement, in particular to a nano-probe used for biological micro-area and photothermia and its synthesis method and detection method. Background technique [0002] At present, there are many temperature measurement techniques, such as thermocouple method, thermal expansion method, near-infrared method and so on. These temperature measurement methods are for local and large-scale temperature measurement, and near-infrared methods cannot distinguish depth information, and thermocouples and other methods require contact measurement and other requirements. These characteristics and requirements limit their use in biomedical precision detection and treatment. in the application. In addition to high stability and accuracy, temperature measurement in biomedicine also has many other special requirements, such as the need to measure the temperature of micro-reg...

Claims

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Application Information

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IPC IPC(8): C09K11/85C09K11/02A61K49/00G01K11/20G01K13/00
CPCC09K11/7773G01K11/20B82Y20/00B82Y40/00A61N5/0613
Inventor 魏言春权莉王益民潘长江刘莹雍建芳
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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