Compound preparation for protecting liver and strengthening intestines in Microptenus salmoides culturing and preparation method thereof
A compound preparation, liver and intestinal health technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria used in food preparation, etc. Many other problems, to achieve the effect of improving intestinal vitality, removing lipid accumulation, and long shelf life
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[0036] Example 1 A compound preparation for protecting liver and intestines in California sea bass farming, and its component ratio is calculated in parts by weight:
[0037] Take 6 parts of bile acid (90% effective content), 8 parts of lauric acid monoglyceride (food grade), and 86 parts of microbial solid fermented product. The above raw material components are weighed in proportion and then mixed uniformly at room temperature, sub-packaged, and finally packaged and stored.
[0038] The said microbial solid fermentation product adopts the following process steps:
[0039] (1) Cultivation of Lactobacillus rhamnosus: Take a ring of Lactobacillus rhamnosus ( Lactobacillus rhamnosus , CGMCC 1.12734, provided by China Common Microbial Culture Collection and Management Center) The lawn is inoculated into 150 mL sterilized MRS liquid medium, and it is cultured at 35 ℃ for 36 h. Then it was put into 2L of sterilized MRS liquid medium and allowed to stand for 36 hours at 35°C to prepare ...
Example Embodiment
[0045] Example 2: A compound preparation for protecting liver and intestines in California sea bass farming, and its component ratio is calculated in parts by weight:
[0046] Take 7 parts of bile acid (effective content 90%), 8 parts of lauric acid monoglyceride (food grade), and 85 parts of microbial solid fermented product. The above raw material components are weighed in proportion and then mixed uniformly at room temperature, sub-packaged, and finally packaged and stored.
[0047] The said microbial solid fermentation product adopts the following process steps:
[0048] (1) Cultivation of Lactobacillus rhamnosus: Take a ring of Lactobacillus rhamnosus ( Lactobacillus rhamnosus , CGMCC 1.3724, provided by the China Common Microbial Species Collection and Management Center) The lawn is inoculated into 200 mL sterilized MRS liquid medium, and incubated at 36°C for 32 h. Then it was put into 3L of sterilized MRS liquid medium, and allowed to stand at 36°C for 32h to be cultured a...
Example Embodiment
[0054] Example 3: A composite preparation for protecting liver and intestines in California sea bass farming contains the following components, and the component ratios are calculated in parts by weight:
[0055] Take 6.2 parts of bile acid (effective content 90%), 7.2 parts of lauric acid monoglyceride (food grade), and 86.6 parts of microbial solid fermentation product. The above raw material components are weighed in proportion and then mixed uniformly at room temperature, sub-packaged, and finally packaged and stored.
[0056] The said microbial solid fermentation product adopts the following process steps:
[0057] (1) Cultivation of Lactobacillus rhamnosus: Take a ring of Lactobacillus rhamnosus ( Lactobacillus rhamnosus , ATCC 7469, provided by China Common Microbial Culture Collection and Management Center) The lawn is inoculated into 100 mL of sterilized MRS liquid medium, and it is cultured at 34 ℃ for 35 hours. Then put it into 1 L of sterilized MRS liquid medium, and c...
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