Compound preparation for protecting liver and strengthening intestines in Microptenus salmoides culturing and preparation method thereof

A compound preparation, liver and intestinal health technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria used in food preparation, etc. Many other problems, to achieve the effect of improving intestinal vitality, removing lipid accumulation, and long shelf life

Pending Publication Date: 2019-09-13
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these treatment methods have certain effects, there are many hidden dangers and disadvantages
The use of antibiotics to eliminate pathogenic bacteria in the intestinal tract is a temporary solution but not a permanent cure, which will lead to problems such as antibiotic residues in tissues and drug resistance. After 2020, my country will ban the addition of antibiotics in feed
Similarly, single addition of silymarin, gl

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0036] Example 1 A compound preparation for protecting liver and intestines in California sea bass farming, and its component ratio is calculated in parts by weight:

[0037] Take 6 parts of bile acid (90% effective content), 8 parts of lauric acid monoglyceride (food grade), and 86 parts of microbial solid fermented product. The above raw material components are weighed in proportion and then mixed uniformly at room temperature, sub-packaged, and finally packaged and stored.

[0038] The said microbial solid fermentation product adopts the following process steps:

[0039] (1) Cultivation of Lactobacillus rhamnosus: Take a ring of Lactobacillus rhamnosus ( Lactobacillus rhamnosus , CGMCC 1.12734, provided by China Common Microbial Culture Collection and Management Center) The lawn is inoculated into 150 mL sterilized MRS liquid medium, and it is cultured at 35 ℃ for 36 h. Then it was put into 2L of sterilized MRS liquid medium and allowed to stand for 36 hours at 35°C to prepare ...

Example Embodiment

[0045] Example 2: A compound preparation for protecting liver and intestines in California sea bass farming, and its component ratio is calculated in parts by weight:

[0046] Take 7 parts of bile acid (effective content 90%), 8 parts of lauric acid monoglyceride (food grade), and 85 parts of microbial solid fermented product. The above raw material components are weighed in proportion and then mixed uniformly at room temperature, sub-packaged, and finally packaged and stored.

[0047] The said microbial solid fermentation product adopts the following process steps:

[0048] (1) Cultivation of Lactobacillus rhamnosus: Take a ring of Lactobacillus rhamnosus ( Lactobacillus rhamnosus , CGMCC 1.3724, provided by the China Common Microbial Species Collection and Management Center) The lawn is inoculated into 200 mL sterilized MRS liquid medium, and incubated at 36°C for 32 h. Then it was put into 3L of sterilized MRS liquid medium, and allowed to stand at 36°C for 32h to be cultured a...

Example Embodiment

[0054] Example 3: A composite preparation for protecting liver and intestines in California sea bass farming contains the following components, and the component ratios are calculated in parts by weight:

[0055] Take 6.2 parts of bile acid (effective content 90%), 7.2 parts of lauric acid monoglyceride (food grade), and 86.6 parts of microbial solid fermentation product. The above raw material components are weighed in proportion and then mixed uniformly at room temperature, sub-packaged, and finally packaged and stored.

[0056] The said microbial solid fermentation product adopts the following process steps:

[0057] (1) Cultivation of Lactobacillus rhamnosus: Take a ring of Lactobacillus rhamnosus ( Lactobacillus rhamnosus , ATCC 7469, provided by China Common Microbial Culture Collection and Management Center) The lawn is inoculated into 100 mL of sterilized MRS liquid medium, and it is cultured at 34 ℃ for 35 hours. Then put it into 1 L of sterilized MRS liquid medium, and c...

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Abstract

The invention relates to a compound preparation for protecting the liver and strengthening intestines in the Microptenus salmoides culturing and a preparation method thereof, and belongs to the technical field of aquaculture. The preparation method comprises the following steps: utilizing clostridium butyricum and lactobacillus rhamnosus to form mixed strains; fermenting the mixed strains in a solid material based on eucommia leaves, silybum marianum fruits, bran, wheat middling, rice bran, inorganic salts and the like to generate a mixed fermentation product with higher viable count; drying and crushing the fermentation product at low temperature; mixing the crushed material with glycerol monolaurate and bile acid according to a certain proportion; and performing packaging to form the compound preparation which can protect the liver of the Microptenus salmoides, improve the activity of intestinal tracts, promote digestion, inhibit pathogenic organisms and improve immunity. The preparation has the advantages of high activity, long storage life and convenient use, is directly added into a feed for feeding, protects liver and strengthens intestines through Microptenus salmoides-intestinal wall repair-intestinal microecological balance-bile acid metabolism axis, effectively removes lipid accumulation in the liver of Microptenus salmoides, repairs intestinal wall, ensures the number of beneficial bacteria in intestines, antagonizes infestation of viruses and bacteria, promotes digestion, reduces disease occurrence, and improves breeding yield and quality.

Description

technical field [0001] The invention relates to a compound preparation for protecting the liver and strengthening the intestines in California sea bass culture and a preparation method thereof, belonging to the technical field of aquaculture. Background technique [0002] California Bass ( Micropterus salmoides ), also known as largemouth bass, belongs to the order Perciformes, the family Sunfish, and the genus Perch, native to the Mississippi River system in California, USA. Because of its delicious meat, fast growth and high economic value, artificial breeding is carried out in many countries and regions. In the late 1970s, Taiwan, China was introduced from abroad, and artificial breeding was successful in 1983. In the same year, California perch was introduced to Guangdong from Taiwan, China. At present, domestic breeding areas are mainly concentrated in Huzhou, Zhejiang, Wujiang, Jiangsu, Nanhai, Shunde, Guangdong and other places. In 2017, the total output of perch f...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K20/105A23K20/158A23K50/80C12N1/20C12R1/225C12R1/145
CPCA23K50/80A23K20/105A23K20/158A23K10/18C12N1/20A23V2400/175Y02A40/818
Inventor 吴伟郑尧陆健刘伟陈树桥周国勤
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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