Compound preparation for protecting liver and strengthening intestines in Microptenus salmoides culturing and preparation method thereof
A compound preparation, liver and intestinal health technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria used in food preparation, etc. Many other problems, to achieve the effect of improving intestinal vitality, removing lipid accumulation, and long shelf life
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Embodiment 1
[0036] Example 1 A compound preparation for protecting the liver and strengthening the intestines in California sea bass culture, the proportion of its components is in parts by weight:
[0037] Take 6 parts of bile acid (90% effective content), 8 parts of lauric monoglyceride (food grade), and 86 parts of microbial solid fermentation product. The above raw material components are weighed in proportion, mixed evenly at room temperature, subpackaged, and finally packaged and put into storage.
[0038] The microbial solid fermented product adopts the following process steps:
[0039] (1) Cultivation of Lactobacillus rhamnosus: Take a ring of Lactobacillus rhamnosus ( Lactobacillus rhamnosus , CGMCC 1.12734, provided by the China General Microorganism Culture Collection Management Center), inoculated into 150mL sterilized MRS liquid medium, and cultured at 35 ℃ for 36 h. Then put it into 2L of sterilized MRS liquid medium, and culture it statically at 35°C for 36h, and prepa...
Embodiment 2
[0045] Embodiment 2: a compound preparation for protecting the liver and strengthening the intestines in the culture of California perch, the proportion of its components is calculated in parts by weight:
[0046] Take 7 parts of bile acid (90% effective content), 8 parts of lauric monoglyceride (food grade), and 85 parts of microbial solid fermentation product. The above raw material components are weighed in proportion, mixed evenly at room temperature, subpackaged, and finally packaged and put into storage.
[0047] The microbial solid fermented product adopts the following process steps:
[0048] (1) Cultivation of Lactobacillus rhamnosus: Take a ring of Lactobacillus rhamnosus ( Lactobacillus rhamnosus , CGMCC 1.3724, provided by the China General Microorganism Culture Collection Management Center) bacterial lawn, inoculated into 200 mL of sterilized MRS liquid medium, and cultured at 36 °C for 32 h. Then it was inserted into 3L of sterilized MRS liquid medium, and c...
Embodiment 3
[0054] Embodiment 3: A compound preparation for protecting the liver and strengthening the intestines in the culture of perch in California comprises the following components, and the proportions of the components are in parts by weight:
[0055] Take 6.2 parts of bile acid (90% effective content), 7.2 parts of lauric monoglyceride (food grade), and 86.6 parts of microbial solid fermentation product. The above raw material components are weighed in proportion, mixed evenly at room temperature, subpackaged, and finally packaged and put into storage.
[0056] The microbial solid fermented product adopts the following process steps:
[0057] (1) Cultivation of Lactobacillus rhamnosus: Take a ring of Lactobacillus rhamnosus ( Lactobacillus rhamnosus , ATCC 7469, provided by China Ordinary Microorganism Culture Collection Management Center) bacterial lawn, inoculated into 100 mL of sterilized MRS liquid medium, and cultured at 34 ℃ for 35 hours. Then put it into 1 L of sterili...
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