Primers for specific gene of Escherichia coli and detection method of Escherichia coli

An Escherichia coli and specific gene technology, applied in the biological field of Escherichia coli, can solve the problems of serious cross-reaction, easy contamination of immunological methods, inability to detect damaged bacteria and dead bacteria, etc., and achieves sensitive and specific test results, convenient and fast detection, Detect quick and cheap effects
CN110229916AInactive Publication Date: 2019-09-13KUNMING UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
KUNMING UNIV OF SCI & TECH
Publication Date
2019-09-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for detecting Escherichia coli on basis of a specific gene of Escherichia coli. The specific gene of Escherichia coli is a hypothetical protein gene which has been registered in Genbank under the registration number 13702648; the specific gene widely exists in Escherichia coli, and has no obvious similarity with other species; and the specific gene has the characteristics of intraspecific commonality and interspecific specificity. Four primers, including a pair of external primers B3 and F3 as well as a pair of internal primers BIP and FIP, are designed on basis of the gene; and then, the primers are applied in loop-mediated isothermal amplification (LAMP), and the external primers B3 and F3 are further applied in polymerase chain reaction (PCR). The nucleotide sequence of the primers is shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4. Identification systems of two kinds of Escherichia coli, namely a PCR reaction system and a LAMP reaction system, are established according to the primers involved; and thus, the identification systems have the characteristics of being good in specificity, high in sensitivity, rapid and reliable indetection result as well as easy to operate. The detection method of Escherichia coli is used for detecting Escherichia coli in water, foods, beverages and cosmetics.
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Description

technical field

[0001] The invention belongs to the technical field of Escherichia coli, and more specifically relates to a method for detecting Escherichia coli based on specific genes of Escherichia coli. Background technique

[0002] Escherichia coli (Escherichia coli), also known as Escherichia coli, is classified in the Enterobacteriaceae family and belongs to the genus Escherichia. Most Escherichia coli are normal resident bacteria in the intestinal tract of humans and animals. Escherichia coli is continuously excreted with feces, polluting the surrounding environment, water sources, food, etc. When taking a sample for inspection, the more E. coli in the sample, the more serious the sample is contaminated by feces, and the greater the possibility of the presence of enteric pathogenic bacteria in the sample. Therefore, hygienic bacteriological inspections should be carried out for drinking water, food, and beverages. E. coli infection can be contracted by drinking con...

Claims

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