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Bacillus megaterium and application thereof in fermentation detoxification of Jatropha curcas L. seed meal

A technology of Bacillus megaterium and Jatropha curcas cake, which is applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of complex biological functions, too many samples, and long cycle, so as to improve nutritional value and enhance The effect of nutritional value

Active Publication Date: 2019-09-17
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(2) The combined toxicity of various toxic components in the degradation process is not considered: when multiple toxins exist in raw materials at the same time, their effects on organisms are far more complex than the effects of a single poison
Existing literature has shown that the degradation product of phorbol ester without side chain mother core structure has the same biological toxicity
(3) Lack of corresponding research on the safety of virus-free strains: Although traditional instrumental detection and analysis are relatively sensitive, there are still multi-index analysis for the detection of toxins produced by microorganisms, and it is impossible to identify undiscovered or difficult-to-detect substances and other shortcomings
[0005] The experimental animals reported in the existing literature to evaluate the toxic components in jatropha seed meal include goats, sheep, chickens, cows, mice, rats, etc. The biological evaluation method is simple and intuitive, but the cycle is too long, and the required samples Too much is not conducive to the rapid and effective conduct of the test

Method used

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  • Bacillus megaterium and application thereof in fermentation detoxification of Jatropha curcas L. seed meal
  • Bacillus megaterium and application thereof in fermentation detoxification of Jatropha curcas L. seed meal
  • Bacillus megaterium and application thereof in fermentation detoxification of Jatropha curcas L. seed meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Screening of Virus-free Strain in Jatropha Seed Cake Fermentation

[0039] one. Materials and Instruments

[0040] 1. Materials

[0041] Jatropha seed cake: the physically pressed jatropha seed cake purchased from Yunnan Shenyu New Energy Co., Ltd. was crushed and passed through a 60-mesh sieve.

[0042] Zebrafish ( Danio rerio ): Bought from Jincheng Aquarium, a famous city of tripod rhinoceros in Pixian County, Chengdu.

[0043] Primary screening and acclimation plate medium: Jatropha seed cake 100g, agar 15g, distilled water 1000ml, natural pH.

[0044] Beef extract peptone agar medium: 3g beef extract, 10g peptone, 5g NaCl, 15g agar, 1000ml distilled water, pH7.4-7.6.

[0045] Beef extract peptone liquid medium: 3g beef extract, 10g peptone, 5g NaCl, 1000 ml distilled water, pH7.4-7.6.

[0046] Solid-state fermentation medium: Weigh 30g jatropha seed cake into a 250mL Erlenmeyer flask, and adjust the initial water content according to the different screening co...

Embodiment 2

[0105] Single factor investigation of solid-state fermentation of jatropha seed cake by Bacillus megaterium SCYA10

[0106] one. Test method and steps

[0107] 1. Strain Preparation

[0108] Inoculate 1 loop of SCYA10 strain slant into beef extract peptone medium, and cultivate at 37°C and 180r / min constant temperature shaking for 16h.

[0109] 2. Determination of growth curve and determination of inoculation time

[0110] Use the uninoculated liquid medium as a blank, and measure the absorbance value of the bacterial solution at different culture times at a wavelength of 600nm. The growth curve of SCYA10 was drawn with the culture time as the abscissa and the absorbance value of the bacterial solution as the ordinate. The inoculum of the subsequent solid-state fermentation is a culture in the logarithmic growth phase.

[0111] The specific operation is as follows:

[0112] (1) Take 63 250ml sterile Erlenmeyer flasks and mark the culture time respectively, namely 0, 2, 4...

Embodiment 3

[0141] Optimizing Fermentation Conditions by Orthogonal Test

[0142] On the basis of the single factor experiment, an orthogonal design of 4 factors and 3 levels was carried out on the four factors of inoculation amount, material-water ratio, fermentation temperature and fermentation time, and the fermentation conditions were further optimized, with 5 repetitions in each group. Orthogonal test factor level table is shown in Table 2. After fermentation, the survival rate of zebrafish was tested with the methanol extracts of fermented cakes of each group. Orthogonal test analysis results are shown in Table 3, 4 and Figure 12 .

[0143] Table 2 Orthogonal test factor level table

[0144] .

[0145] Table 3 Range analysis table of fermentation orthogonal test

[0146] .

[0147] Table 4 Analysis of variance table of fermentation orthogonal test

[0148] .

[0149] From the R value of the zebrafish survival rate in the range analysis (Table 3), it can be seen that t...

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Abstract

The invention discloses bacillus megaterium with a preservation number of CCTCC NO: M2019448, preserved by China Center for Type Culture Collection. The invention also discloses an application of the bacillus megaterium in the fermentation detoxification of Jatropha curcas L. seed meal. The bacillus megaterium, with the preservation number of CCTCC NO: M2019448, can effectively reduce toxic components of the Jatropha curcas L. seed meal and improve application value of the Jatropha curcas L. seed meal.

Description

technical field [0001] The invention belongs to the field of fermentation, and in particular relates to a strain of bacillus megaterium and its application in the fermentation and detoxification of jatropha seed cake. Background technique [0002] Jatropha curcas is a kind of bioenergy tree species, which is widely planted in Southwest my country. The annual output of jatropha seed cake after oil extraction is nearly one million tons and is rich in protein, which has a good potential to be developed as a new protein feed resource. However, it contains phorbol and its derivatives, jatrophenol and other toxic components that are difficult to remove. Direct feeding to animals will cause diarrhea, dehydration, bleeding in some organs and even death. The existence of these toxic components restricts the utilization of jatropha seed meal. If its toxicity can be reduced, it can be used as feed protein to alleviate the increasing shortage of protein feed raw materials in the aquac...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L5/20C12R1/11
CPCA23L5/28C12N1/205C12R2001/11
Inventor 贾刚张珍誉赵华陈小玲刘光芒徐严
Owner SICHUAN AGRI UNIV
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