Bacillus megaterium and application thereof in fermentation detoxification of Jatropha curcas L. seed meal

A technology of Bacillus megaterium and Jatropha curcas cake, which is applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of complex biological functions, too many samples, and long cycle, so as to improve nutritional value and enhance The effect of nutritional value

Active Publication Date: 2019-09-17
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(2) The combined toxicity of various toxic components in the degradation process is not considered: when multiple toxins exist in raw materials at the same time, their effects on organisms are far more complex than the effects of a single poison
Existing literature has shown that the degradation product of phorbol ester without side chain mother core structure has the same biological toxicity
(3) Lack of corresponding research on the safety of virus-free strains: Although traditional instrumental detection and analysis are relatively sensitive, ther

Method used

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  • Bacillus megaterium and application thereof in fermentation detoxification of Jatropha curcas L. seed meal
  • Bacillus megaterium and application thereof in fermentation detoxification of Jatropha curcas L. seed meal
  • Bacillus megaterium and application thereof in fermentation detoxification of Jatropha curcas L. seed meal

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0037] Example 1

[0038] Screening of Detoxification Strains for Fermentation of Jatropha seed Cake

[0039] One. Materials and instruments

[0040] 1. Materials

[0041] Jatropha seed cake: The physically pressed jatropha seed cake purchased from Yunnan Shenyu New Energy Co., Ltd. is crushed through a 60-mesh sieve, and then obtained.

[0042] Zebrafish ( Danio rerio ): Purchased from Jincheng Aquarium, Dingxi City, Pixian County, Chengdu.

[0043] Preliminary screening and acclimatization of plate medium: 100g jatropha seed meal, 15g agar, 1000ml distilled water, natural pH.

[0044] Beef extract peptone agar medium: beef extract 3g, peptone 10g, NaCl 5g, agar 15g, distilled water 1000ml, pH 7.4-7.6.

[0045] Beef extract peptone liquid medium: beef extract 3g, peptone 10g, NaCl 5g, distilled water 1000 ml, pH 7.4-7.6.

[0046] Solid-state fermentation medium: Weigh 30g of jatropha seed cake in a 250mL Erlenmeyer flask, and adjust the initial water content according to the different sc...

Example Embodiment

[0104] Example 2

[0105] Single-factor investigation of solid-state fermentation of jatropha seed meal with Bacillus megaterium SCYA10

[0106] One. Test method and procedure

[0107] 1. Strain preparation

[0108] One loop of SCYA10 strain was inoculated into the beef extract peptone medium, and the culture was shaken at a constant temperature of 37°C and 180r / min for 16 hours.

[0109] 2. Determination of growth curve and determination of inoculation time

[0110] Using the uninoculated liquid culture medium as a blank, the absorbance value of the bacterial solution under different culture time was measured at a wavelength of 600nm. Draw the growth curve of SCYA10 with the culture time as the abscissa and the absorbance of the bacterial solution as the ordinate. The inoculum for subsequent solid-state fermentation is a logarithmic growth phase culture.

[0111] The specific operations are as follows:

[0112] (1) Take 63 250ml sterile Erlenmeyer flasks and mark the culture time respe...

Example Embodiment

[0140] Example 3

[0141] Orthogonal test to optimize fermentation conditions

[0142] On the basis of the single factor experiment, a 4-factor 3-level orthogonal design was carried out for the four factors of inoculation amount, material-water ratio, fermentation temperature, and fermentation time to further optimize the fermentation conditions, with 5 replicates in each group. The orthogonal test factor level table is shown in Table 2. After the fermentation, the methanol extracts of each group of fermented cakes were used to test the survival rate of zebrafish. Orthogonal test analysis results are shown in Tables 3, 4 and Picture 12 .

[0143] Table 2 Orthogonal test factor level table

[0144] .

[0145] Table 3 Range analysis table of fermentation orthogonal test

[0146] .

[0147] Table 4 Analysis of variance of fermentation orthogonal test

[0148] .

[0149] From the range analysis (Table 3), the R value of zebrafish survival rate shows that the order of the factors affectin...

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Abstract

The invention discloses bacillus megaterium with a preservation number of CCTCC NO: M2019448, preserved by China Center for Type Culture Collection. The invention also discloses an application of the bacillus megaterium in the fermentation detoxification of Jatropha curcas L. seed meal. The bacillus megaterium, with the preservation number of CCTCC NO: M2019448, can effectively reduce toxic components of the Jatropha curcas L. seed meal and improve application value of the Jatropha curcas L. seed meal.

Description

technical field [0001] The invention belongs to the field of fermentation, and in particular relates to a strain of bacillus megaterium and its application in the fermentation and detoxification of jatropha seed cake. Background technique [0002] Jatropha curcas is a kind of bioenergy tree species, which is widely planted in Southwest my country. The annual output of jatropha seed cake after oil extraction is nearly one million tons and is rich in protein, which has a good potential to be developed as a new protein feed resource. However, it contains phorbol and its derivatives, jatrophenol and other toxic components that are difficult to remove. Direct feeding to animals will cause diarrhea, dehydration, bleeding in some organs and even death. The existence of these toxic components restricts the utilization of jatropha seed meal. If its toxicity can be reduced, it can be used as feed protein to alleviate the increasing shortage of protein feed raw materials in the aquac...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L5/20C12R1/11
CPCA23L5/28C12N1/205C12R2001/11
Inventor 贾刚张珍誉赵华陈小玲刘光芒徐严
Owner SICHUAN AGRI UNIV
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