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A Visual Sensing Method for Chloride Ion Detection in Sweat

A technology for chloride ions and sweat, applied in the field of visual sensing to detect chloride ions in sweat, can solve problems such as complex construction, achieve good reproducibility, easy operation, and high sensitivity

Inactive Publication Date: 2021-12-03
XIAMEN HUAXIA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to improve the accuracy of the method, it is often necessary to introduce a reference into the fluorescence system to construct a ratiometric fluorescence detection method. However, the construction of these ratiometric fluorescence sensing systems is often relatively complicated. Therefore, the development of new Cl - Fluorescent sensing materials, and create an efficient and accurate chloride ion sensing system for Cl in sweat - The rapid detection of

Method used

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  • A Visual Sensing Method for Chloride Ion Detection in Sweat
  • A Visual Sensing Method for Chloride Ion Detection in Sweat
  • A Visual Sensing Method for Chloride Ion Detection in Sweat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Preparation of perovskite nanocrystals (CsPbBr 3 PNCs), the steps are: 5mL ODE, 0.75mL OAm, 0.016gCs 2 CO 3 And 0.0387g PbSTR, 0.071g TBB were added into a 25mL three-neck flask in turn, stirred vigorously at 2500rpm, and directly heated to 160°C in air, and finally cooled naturally.

[0055] The obtained primary product was washed with a mixed solvent of ethyl acetate and n-hexane (V ethyl acetate / V n-hexane = 3:1), the rotation speed was 10000 rpm, the centrifugation time was 10 min, and the washing and purification were performed three times. The bottom product was taken and discarded Clear liquid, and disperse the final product in n-hexane solvent for later use.

[0056] Take 1mL of the above CsPbBr 3 PNCs solution, add 5 μL NaCl aqueous solution (10-130 mM) of different concentrations, and then add 5 μL 0.1M H 2 SO4 dilute sulfuric acid solution. After stirring at 1500rpm for 5min, select the excitation wavelength of 365nm, and collect CsPbBr with F-7100 fluor...

Embodiment 2

[0062] In order to obtain the best sensing performance, the experiment investigated the influence of reaction conditions solvent pH, stirring speed and reaction temperature, and CsPbBr 3 The above conditions were optimized for the wavelength shift of PNCs after adding 60mM NaCl to the reaction.

[0063] The experiment explored the effect of pH on the sensing system.

[0064] Experimental results such as image 3 As shown, the experiment found that under magnetic stirring, only when the medium pH=1, the Cl in the water phase - Fangneng and CsPbBr dispersed in n-hexane 3 PNCs perform halogen exchange, but it is difficult to perform effective halogen exchange under other pH conditions. The reason for this phenomenon may be that Cl - Protonation under strong acidic conditions forms HCl, which facilitates its diffusion into n-hexane solution, and then combines with CsPbBr 3 PNCs undergo rapid halogen exchange, and it is difficult to effectively convert Cl- to HCl at other pHs,...

Embodiment 3

[0068] In order to investigate the selectivity of the method, an interference experiment was carried out before the actual sample analysis.

[0069] The experiment selected common substances or similar substances in sweat such as NaF, NaBr, NaI, K 2 SO 4 , MgSO 4 , Ca(NO 3 ) 2 , Fe(NO 3 ) 3 、Na 3 PO 4 、Na 2 CO 3 , MgSO 4 , NaHCO 3 , NaH 2 PO 4 , urea, ammonia, glucose, arginine and lactic acid, etc. for interference experiments. Interference experiments The sensing and detection of interfering substances was carried out as described in Example 1.

[0070] The results of interference experiments are as follows Figure 5 shown. Based on CsPbBr 3 PNCs and Cl - Halogen exchange achieved for Cl in sweat - Fluorescence visual sensing has good selectivity, the reasons are as follows: (1) NaCl is the main component in sweat (average level is about 23mM), which is higher than other components; (2) the halogen exchange specificity. Although the concentration of lact...

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Abstract

The invention discloses a method for visual sensing to detect chloride ions in sweat. The invention firstly prepares a perovskite nanocrystal (CsPbBr) with good luminescent properties and halogen exchange properties. 3 PNCs), and based on the fluorescence characteristics and halogen exchange characteristics of the material itself, it is used as a sensing material to detect chloride ions in sweat. The present invention makes full use of CsPbBr 3 Br in PNCs ‑ with Cl in the aqueous phase ‑ Halogen exchange under heterogeneous conditions, obvious wavelength shift, and visual sensing characteristics, combined with the advantages of high sensitivity and detection of fluorescence spectroscopy, provide a convenient, simple, no-sample pre-treatment condition, and easy to popularize and use Chlorine in sweat Ion determination method.

Description

technical field [0001] The invention relates to a method for visually sensing and detecting chloride ions in sweat. Background technique [0002] Sweat is the filtrate of serum and contains electrolytes (K + 、Na + , Cl - ) and metabolites (urea, lactic acid, etc.), etc., whose component identification and content determination can be used for the prediction and diagnosis and treatment evaluation of human diseases, so the detection of the substance content has attracted people's attention. [0003] Cl in sweat - Detection has always been the gold standard for the evaluation of the genetic disease cystic fibrosis (Cystic fibrosis). Based on this standard, researchers have developed a series of Cl - Analytical method of detection. Currently, common Cl - The detection methods mainly include high performance liquid chromatography, ion chromatography, electrochemical method, double-end injection capillary electrophoresis, fluorescence method and colorimetric instant detectio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/6486
Inventor 陈曦郭志勇李飞明姚秋虹
Owner XIAMEN HUAXIA UNIV
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