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Biological surgery patch and preparation method thereof

A surgical and biological technology, applied in medical science, coatings, non-woven fabrics, etc., can solve problems such as long solid fermentation time, insignificant improvement of mechanical properties of membrane materials, unfavorable cell and tissue proliferation, etc.

Active Publication Date: 2019-09-20
NKD PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The existing preparation method of biosurgical patches is direct fermentation, and it is a static fermentation method without adding other ingredients during the fermentation process. The thickness of the bacterial cellulose membrane obtained is controlled according to the length of fermentation time, thereby indirectly adjusting the mechanical properties of the membrane material. , the solid fermentation time is long, the mechanical properties of the membrane material are not significantly improved, and the pore size is dense, which is not conducive to the proliferation of cells and tissues, and it is difficult to meet the clinical needs.

Method used

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  • Biological surgery patch and preparation method thereof
  • Biological surgery patch and preparation method thereof
  • Biological surgery patch and preparation method thereof

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preparation example Construction

[0025] The invention provides a method for preparing a biosurgical patch. The preparation base material is a bacterial cellulose film obtained through biological fermentation; the bacterial cellulose film obtained through biological fermentation is purified, oxidized, and freeze-thawed. , Composite process treatment, electrospinning treatment, freeze-drying treatment and film pressing treatment to obtain biosurgical patch;

[0026] The preparation method of described biosurgical patch comprises the following steps:

[0027] 1) Fermenting and culturing the strains to obtain bacterial cellulose membranes; adding biopolymer materials during the fermentation and culturing; the biopolymer materials include polylactic acid, polyamide, polyester, glycerin, polylactide, poly One or more of caprolactone, polyvinyl chloride, polyethylene;

[0028] 2) Soaking the bacterial cellulose membrane obtained in step 1) in the inorganic alkali solution and the acid solution successively to obtai...

Embodiment 1

[0044] Step 1) Preparation of culture medium: Weigh 20g of mannitol, 15g of polyethylene glycol, 25g of sucrose, 20g of glucose, 0.5mg of niacin, 5g of yeast powder, 10g of corn steep liquor powder, 5g of beef extract, 10g of peptone, KH2 PO 4 2g, MgSO 4 2g, 8g of absolute ethanol, 0.5g of ammonium sulfate, 0.5g of citric acid, 0.5g of sodium carboxymethylcellulose, 10g of gluconic acid, and 40g of sodium gluconate were added to 1000mL of purified water.

[0045] Step 2) Fermentation: Activate the microbial strains that produce cellulose film to obtain activated strains, transfer the activated strains to stainless steel trays for expanded cultivation, obtain seed liquid, and cultivate them in a constant temperature incubator at 37°C for 10 days. On the first day and the third day, 2% mannitol and 5% polyethylene glycol were added respectively, and the pH of the system was maintained at 6.8 during the whole fermentation process, and the oxygen flow rate was 0.4L / min. One layer...

Embodiment 2

[0055] Step 1) Preparation of culture medium: Weigh 25g of mannitol, 10g of polyethylene glycol, 30g of sucrose, 15g of glucose, 0.8mg of niacin, 6g of yeast powder, 15g of corn steep liquor powder, 10g of beef extract, 5g of peptone, KH 2 PO 4 1.5g, MgSO 4 2.5g, 10g of absolute ethanol, 0.8g of ammonium sulfate, 0.6g of citric acid, 0.6g of sodium carboxymethylcellulose, 10g of gluconic acid, and 50g of sodium gluconate were added to 1000mL of purified water.

[0056] Step 2) Fermentation: Activate the microbial strains that produce cellulose film to obtain activated strains, transfer the activated strains to stainless steel trays for expanded cultivation, obtain seed liquid, and cultivate them in a constant temperature incubator at 40°C for 6 days. On the first day and the third day, 2% mannitol and 5% polyethylene glycol were added respectively, and the pH of the system was maintained at 3.8 during the whole fermentation process, and the oxygen flow rate was 3.5L / min. One...

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Abstract

The invention relates to a biological surgery patch and a preparation method thereof and belongs to the technical field of biological materials. The preparation method includes: using a bacterial cellulose membrane prepared by biological fermentation as the base material; subjecting the bacterial cellulose membrane to purification, oxidation, freeze thawing, composite process processing, electrostatic spinning, freeze drying and membrane pressing to obtain the biological surgery patch. The biological surgery patch prepared by the method is good in biocompatibility, mechanical performance, sewing performance and clinical application convenience and capable of satisfying the basic requirements of surgery patch materials.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a method for preparing a biosurgical patch and a biosurgical patch prepared based on the method. Background technique [0002] The existing preparation method of biosurgical patches is direct fermentation, and it is a static fermentation method without adding other ingredients during the fermentation process. The thickness of the bacterial cellulose membrane obtained is controlled according to the length of fermentation time, thereby indirectly adjusting the mechanical properties of the membrane material. , The solid fermentation time is long, the mechanical properties of the membrane material are not significantly improved, and the pore size is dense, which is not conducive to the proliferation of cells and tissues, and it is difficult to meet the clinical needs. Contents of the invention [0003] The object of the present invention is to provide a method for preparing a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/18A61L27/16A61L27/34A61L27/50A61L27/54A61L27/58D04H13/00D04H1/728C12P19/04
CPCA61L27/16A61L27/18A61L27/20A61L27/34A61L27/50A61L27/54A61L27/58A61L2300/404C12P19/04D04H1/728D04H13/00C08L1/02C08L67/04C08L77/00C08L67/02C08L27/06C08L23/06C08L33/20C08L69/00C08L27/16C08L5/08
Inventor 王国才朱翠兰陶秀梅
Owner NKD PHARMA CO LTD
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