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New application of cumin volatile oil

A technology of cumin and volatile oil, applied in the application, chemical for biological control, fungicides and other directions, can solve problems such as environmental pollution, pollute the environment, kill and injure natural enemies, etc., achieve broad application prospects, simple preparation method, and convenient use Effect

Active Publication Date: 2021-06-15
SHENZHEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Chemical agents are preparations that have an inhibitory effect on microorganisms. According to the nature of their action, chemical agents can be divided into bactericides and bacteriostatic agents. Bactericides are chemical agents that can destroy the metabolic function of bacteria and have lethal effects. Bacteriostatic agents do not destroy bacteria. The protoplasm of the protoplasm, but only inhibits the synthesis of new cell substances, so that the bacteria cannot proliferate; the type of microorganisms, the length of time for the chemical agents to treat the microorganisms, the temperature and the environment of the microorganisms, etc., all affect the ability and ability of the chemical agents to sterilize or inhibit bacteria. Effect: Excessive use of chemical agents not only causes economic waste, but also causes drug damage, pollutes the environment, kills natural enemies, is harmful to human health, and has many adverse consequences;
[0003] As a valuable cultivated medicinal plant, notoginseng has become a major obstacle to the production of notoginseng with the increasing social demand and the rapid increase in planting area of ​​notoginseng. Rely on various chemical agents. Although chemical pesticides are effective, they greatly increase the input cost of farmers and reduce the quality of medicinal materials. High residual pesticides will cause serious environmental pollution.
[0006] At present, there is no document disclosure related to the technical solution of the present invention

Method used

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  • New application of cumin volatile oil
  • New application of cumin volatile oil
  • New application of cumin volatile oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Preparation of fennel volatile oil

[0022] (1) Add water 7 times its mass to 1000g cumin, soak for 2 hours, use steam to extract the volatile oil in cumin by heating and distillation, the extraction time is 8 hours, condense and collect the effluent;

[0023] (2) The extracted liquid is dried with anhydrous sodium sulfate to remove the contained water, which is the cumin volatile oil. Put it into a brown sample bottle, seal it, and store it at -20°C for later use.

Embodiment 2

[0024] Embodiment 2: the activation of pathogenic bacteria

[0025] (1) Potato Dextrose Agar Medium (PDA): 200g potato, 20g glucose, 20g agar, 1000mL distilled water;

[0026] (2) Purify and identify the pathogenic bacteria isolated from the roots and shoots of Panax notoginseng, and identify them as Fusarium oxysporum, Fusarium solani, Pythiumaphanidermatum, and Cylindrosporium (Cylindrocarpon destructans), Colletotrichum gloeosporioides (Colletotrichum gloeosporioides) and Rhizoctonia solani (Rhizoctonia solani), the above strains were inoculated on PDA medium for activation, after inoculation 3 to 4 times, the strains with vigorous growth were taken for later use .

Embodiment 3

[0027] Embodiment 3: GC-MS measures the chemical composition of fennel volatile oil

[0028] (1) GC conditions: Instrument model: Agilengt Technologies 7890B-5977B; Chromatographic column: HP-5MS30m×250μm×0.25μm;

[0029] (2) EI source ionization source temperature: 230°C; quadrupole temperature: 150°C; scanning range: 30-500m / z; inlet temperature: 285°C; split ratio 10:1; sample volume: 1μl; Energy: 70eV; temperature program of column oven: 50°C for 4min, 5°C / min to 120°C, 1°C / min to 180°C, 10°C / min to 280°C and hold for 16min; The retention time of the alkane continuous carbon (C9-C25) is calculated, and the retention time and mass spectrum of the compound are compared with the NIST17.L database to determine the final compound. The test results are shown in the table below;

[0030]

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Abstract

The invention discloses a new agricultural application of cumin volatile oil; namely, its application in preventing and treating Chinese medicinal materials or crop root rot, or in preventing and controlling pathogenic bacteria that cause diseases of traditional Chinese medicinal materials or crop shoots; The experimental results showed that the volatile oil of cumin had significant effects on the four main pathogenic bacteria, Fusarium oxysporum, Fusarium rot, Pythium and Cylindrosporium, which caused the root rot of Panax notoginseng, and the two species that caused the root rot of Panax notoginseng. The mycelium growth of the pathogenic bacteria Glycystis anthracis and Rhizoctonia solani has a strong inhibitory effect. When the concentration is 50 mg / mL, the inhibition rate is above 80%. Among them, the pathogenic bacteria of Panax notoginseng aboveground diseases The inhibitory effect reached 100%; the MIC results showed that the volatile oil of cumin had a good antifungal effect on the above strains. Through the analysis of the FIC value, it was found that the volatile oil of cumin and oxamid were combined with a variety of pathogens. In addition, the volatile oil is volatile and has the characteristics of fragrant and foul-smelling, so the fertilizer or pesticide developed with it will have the advantages of safety, effectiveness and low residue.

Description

technical field [0001] The invention relates to the use of fennel volatile oil, in particular to the control effect of cumin volatile oil on Chinese medicinal materials or crop root rot and various diseases of Chinese medicinal materials or aboveground parts of crops, and belongs to the technical field of biological pesticides. Background technique [0002] Chemical agents are preparations that have an inhibitory effect on microorganisms. According to the nature of their action, chemical agents can be divided into bactericides and bacteriostatic agents. Bactericides are chemical agents that can destroy the metabolic function of bacteria and have lethal effects. Bacteriostatic agents do not destroy bacteria. The protoplasm of the protoplasm, but only inhibits the synthesis of new cell substances, so that the bacteria cannot proliferate; the type of microorganisms, the length of time for the chemical agents to treat the microorganisms, the temperature and the environment of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/10A01N43/80A01P3/00
CPCA01N43/80A01N65/00A01N65/10
Inventor 程永现董鲜
Owner SHENZHEN UNIV