Modified bacterial cellulose composite scaffold material for umbilical cord MSCs and preparation method thereof

Abstract

The invention discloses a modified bacterial cellulose composite scaffold material for umbilical cord MSCs and a preparation method thereof, and belongs to the field of stem cell transplantation and the field of bacterial cellulose composite scaffold materials. According to the technical scheme, the modified bacterial cellulose composite scaffold material comprises a modified bacterial cellulose composite membrane and the umbilical cord MSCs which are implanted on the modified bacterial cellulose composite membrane. The preparation method comprises the steps that neonatal umbilical cord Wharton's jelly is cultured through an MSCs culture medium to obtain P3 umbilical cord MSCs; after freeze-drying, the physical properties of the composite membrane are determined. According to the preparation method, the bacterial cellulose composite membrane can be used as a scaffold to which the mesenchymal stem cells are attached, during cerebral injury local transplantation, the effect that the mesenchymal stem cells promote neural functional recovery is fully exerted, and the problem about the way how the stem cells are transplanted efficiently is solved; the modified bacterial cellulose composite scaffold material can replace an artificial meningeal patch to recover defective cerebral dura mater, the meningeal integrity is reconstructed, brain tissue is protected, and leakage of cerebrospinal fluid, intracranial infection, encephalocele, epilepsy and other complications are prevented.

Description

technical field [0001] The invention relates to the field of stem cell transplantation and the technical field of bacterial cellulose composite scaffold material, in particular to a bacterial cellulose composite scaffold material modified by umbilical cord MSCs and a preparation method thereof. Background technique [0002] Hypertensive intracerebral hemorrhage is a global disease with high morbidity, disability and mortality, and is a common and serious disease that endangers human health. The annual incidence of cerebral hemorrhage in my country is as high as 50.6 / 100,000 to 80.7 / 100,000. In recent years, with the acceleration of the population aging process and the change of lifestyle, the incidence rate has been increasing year by year. The acute onset, poor prognosis and high medical expenses seriously endanger the health and quality of life. It is a difficult clinical and social public health problem. question. Traditional medical conservative treatment or craniotomy...

AI Technical Summary

Problems solved by technology

[0004] Clinical research on the application of dura mater materials began in the early 20th century, but an ideal artificial dura mater replacement material has not yet been developed

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