Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Anthracene-thiosemicarbazide derivative, preparation method thereof and application of derivative as fluorescent probe

A technology of thiosemicarbazide and derivatives, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., to achieve the effects of low excitation energy, small cell damage, and improved signal-to-noise ratio

Active Publication Date: 2019-09-27
SUZHOU UNIV OF SCI & TECH
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on ion probes that can achieve upconversion fluorescence quenching response in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Anthracene-thiosemicarbazide derivative, preparation method thereof and application of derivative as fluorescent probe
  • Anthracene-thiosemicarbazide derivative, preparation method thereof and application of derivative as fluorescent probe
  • Anthracene-thiosemicarbazide derivative, preparation method thereof and application of derivative as fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] In a 250ml three-necked flask, add 4-bromo-2-hydroxybenzaldehyde (2.84g, 11.19mmol), 9,10-diborate anthracene (2g, 4.73mmol) dissolved in 100ml 1,4-dioxane and 24ml ethanol, K 2 CO 3 (3.92 g, 28.38 mmol) was dissolved in 48 mL of distilled water and mixed into the above solution. Then, nitrogen gas was bubbled into the mixed solution for 15 minutes, and then tetrakis(triphenylphosphine) palladium (0) (0.39 g, 1.2 mmol) was added, and nitrogen gas was bubbled in for another 5 minutes, and the reaction was carried out at 120°C in a nitrogen atmosphere. During the reaction, The progress of the reaction was tracked by spotting the plate. The developer was dichloromethane:petroleum ether=1:1. After 48 hours of reaction, the spot of raw material 9,10-diborate anthracene almost disappeared, and the reaction was stopped. After the reaction was over, the reaction solution was distilled under reduced pressure to obtain a black solid mixture, and the organic phase was extracted...

Embodiment 2

[0039] The probe molecule of Example 1 was added to DMF, and the probe molecule DMF mother solution was prepared with a concentration of 1 mM.

[0040] Add 1.97 mL DMF solution and 1 mL buffered aqueous solution (the buffer solution is Na 2 HPO 4 / NaH 2 PO 4 , pH is 7, 1 / 2, v / v), then take 30 μL probe molecule DMF mother solution (concentration: 1mM) and add it to the above-mentioned quartz cuvette, ultrasonically dissolve it, and then prepare a 10 μM probe molecule Needle molecule solution.

[0041] Similarly, add 1.7 mL DMF solution and 1 mL buffered aqueous solution (Na 2 HPO 4 / NaH 2 PO 4 , pH is 7, 1 / 2, v / v), then take 300 μL of probe molecule DMF mother solution (concentration: 1mM) and add it to the above-mentioned quartz cuvette, ultrasonically dissolve it, and then prepare a 100 μM probe molecule Needle molecule solution.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses an anthracene-thiosemicarbazide derivative, a preparation method thereof and application of the derivative as a fluorescent probe. The preparation method comprises the following steps: 4-bromo-2-hydroxybenzaldehyde reacts with 9,10-diborate anthracene in a nitrogen atmosphere and in an organic solvent to obtain an intermediate product I; and then the intermediate product I reacts with thiosemicarbazide to obtain a copper ion fluorescent probe. The fluorescent probe of the invention has efficient up-conversion and down-conversion fluorescence quenching response recognition characteristics of copper ions.

Description

technical field [0001] The invention belongs to the field of fluorescent reagents and the technical field of fluorescent probes for heavy metal ions, and specifically relates to an anthracene-thiosemicarbazide derivative probe and a preparation method thereof, and the application of the probe in detecting copper ions in water environments or organisms. The probe has the characteristic of upconverting fluorescence response. Background technique [0002] Copper ions (Cu 2+ ) Pollution has many hazards, it will not only affect the growth of animals and plants, microorganisms and the activity of soil enzymes, but also destroy the balance of the ecosystem. It will also accumulate in animals and plants, increase the toxicity of copper, and have adverse effects on human health. Detection of Cu 2+ has important practical significance. At present, there are many methods for the determination of copper ions, mainly including: spectrophotometry, fluorescence analysis, electrochemic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07C337/08C09K11/06G01N21/64
CPCC09K11/06G01N21/643C09K2211/1007C09K2211/1011G01N2021/6432C07C335/40
Inventor 王筱梅黄苏琴宋飒飒朱琳叶常青
Owner SUZHOU UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com