A quantitative detection method for Staphylococcus aureus based on sers technology
A quantitative detection method, Staphylococcus technology, applied in the direction of microbial-based methods, microbial determination/inspection, biochemical equipment and methods, etc., can solve complex and time-consuming synthesis of nanoprobes, expensive antibodies or biological enzymes, bacterial signal Weakness and other problems, to achieve the effect of stable enrichment effect, short preparation time and strong catalytic ability
Active Publication Date: 2022-02-15
JIANGSU UNIV
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Problems solved by technology
Although the previously reported SERS method can achieve rapid detection of bacteria, there are still problems such as complex and time-consuming synthesis of nanoprobes and low reproducibility, expensive antibodies or biological enzymes that are easily inactivated, and weak signals of some bacteria that lead to high detection limits.
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[0025] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0026] The first step, preparation of materials:
[0027] Preparation of negatively charged magnetic capture probes: Weigh 3.4g FeCl 3 ·6H 2 O. 6g of sodium acetate, add 100ml of ethylene glycol and stir to make it evenly mixed, add 1g of trisodium citrate dihydrate, and ultrasonically dissolve it for 30 minutes, then introduce the mixed solution into a polyethylene tetrafluoro autoclave, and react at 200°C After cooling to room temperature for 10 hours, wash with ethanol and pure water several times, and then vacuum-dry at 60°C for 12 hours.
[0028] Preparation of Fe-MIL-88 nanozyme: 0.115g (0...
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The invention discloses a method for quantitative detection of Staphylococcus aureus based on SERS technology. A magnetic capture probe is added to the suspension of Staphylococcus aureus to be tested. After mixing, magnetic separation discards the supernatant, and an appropriate amount of suitable Body suspension, shake well and incubate for a period of time, magnetically separate and take the supernatant, then add an appropriate amount of Fe-MIL-88 nanozyme, incubate for a period of time, add a small amount of buffer, H 2 O 2 and colorless malachite green, incubate for a period of time, when the catalytic reaction is over, add a SERS enhancer, after obtaining the Raman signal, establish a standard curve between the Raman signal and the concentration of Staphylococcus aureus, so as to realize the detection of Staphylococcus aureus Quantitative detection, the method has low cost, high sensitivity, strong reliability, and fast detection speed, and is suitable for technical fields such as food safety, environmental detection, and blood analysis.
Description
technical field [0001] The invention belongs to the technical field of SERS and staphylococcus aureus detection, in particular to a method for quantitative detection of staphylococcus aureus based on SERS technology. Background technique [0002] Staphylococcus aureus (Staphylococcus aureus) is an important pathogen. The enterotoxin secreted by it has strong resistance to high temperature, acid, protease hydrolysis and anti-drying properties. cause food poisoning. In my country, food poisoning caused by Staphylococcus aureus accounts for as high as 20% to 25% of all bacterial food poisoning. The main symptoms of poisoning are vomiting, fever and diarrhea. Staphylococcus aureus infection can cause local suppurative infection, pneumonia, pseudomembranous enteritis, pericarditis, etc., and even systemic infection such as sepsis and sepsis. [0003] Traditional detection methods of Staphylococcus aureus mainly include three categories: biological methods, PCR methods, and enz...
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IPC IPC(8): G01N21/65G01N33/58C12Q1/26C12Q1/14C12R1/445
CPCG01N21/658G01N33/587C12Q1/26C12Q1/14
Inventor 陈全胜刘双双李欢欢贺培欢
Owner JIANGSU UNIV