Soluble kelp powder containing brown algae oligosaccharide and enzyme method production method of soluble kelp powder

A technology of brown algae oligosaccharide and kelp powder is applied in the directions of food forming, food science, application, etc., can solve the problems of low yield of brown algae oligosaccharide, extensive process, pollute the environment, etc. Yield-enhancing effect

Inactive Publication Date: 2019-10-08
山东海之宝海洋科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the problems of extensive process, low yield of fucoidan oligosaccharides, environmental pollution and long time consumption in the current kelp processing, and provides an enzyme for directly preparing soluble kelp powder rich in fucoidan oligosaccharides from fresh kelp (or kelp leftovers) production method, the process steps are simplified, the reaction conditions are mild, the cost is low, and the yield is high

Method used

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  • Soluble kelp powder containing brown algae oligosaccharide and enzyme method production method of soluble kelp powder

Examples

Experimental program
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Effect test

Embodiment 1

[0028] (1) Raw material processing: Weigh fresh kelp (or kelp scraps), soak in clean water for 0.5h, wash away the sediment and mucus, then blanch with hot water at 40-60°C to soften, and then add 3 times the volume Water, beat into kelp slurry with a beater.

[0029] (2) Enzymolysis: put the kelp pulp described in step (1) into the enzymolysis tank, add alginate lyase with a wet weight of 1‰ of kelp (prepared by the method described in Example 2) and 1% Cellulase, enzymolysis for 2-4 hours to obtain enzymolysis reaction slurry, the reaction temperature is 35-40°C, pH 6.5-7.5, NaCl concentration 300mM. The specific enzyme activity of cellulase is 10,000 U / g, and the specific enzyme activity of alginate lyase is 20,000 U / g.

[0030] (3) Centrifugal filtration: centrifuge (13000g) the enzymolysis reaction slurry described in step (2) with a centrifuge to obtain a supernatant containing fucoidan oligosaccharides and other soluble components, and the residue is used to produce se...

Embodiment 2

[0035] The preparation method of embodiment 2 alginate lyase

[0036] A Screening Method for Vibrio natriegens

[0037] (1) The sea mud was sampled near the kelp breeding factory in Rongcheng City, Shandong Province, and 1g of the sample was taken and dispersed evenly in 50mL of sterile water.

[0038] (2) Inoculate 1 mL of supernatant into 50 mL of screening liquid medium, culture at 28°C and 200 rpm for 2 days, and dilute 10 -6 And spread it on the screening plate medium, culture at 28°C for 2 days, pick single colonies of different shapes and streak the plates several times to obtain pure cultures.

[0039](3) Pick single colonies of different forms, inoculate them in liquid medium, and culture them at 28°C and 200rpm for 2 days, take the supernatant to measure the enzyme activity of the strain, select a strain with higher enzyme activity, and entrust the China Center for Typical Culture Collection to preserve it. The morphological characteristics, physiological and bioch...

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Abstract

The invention discloses a soluble kelp powder containing brown algae oligosaccharide and an enzyme method production method of the soluble kelp powder, and belongs to the technical field of marine resource processing. The soluble kelp powder containing the brown algae oligosaccharide is directly prepared from fresh kelp (or kelp tail materials). The enzyme method production method of the soluble kelp powder containing the brown algae oligosaccharide comprises the processing steps of raw material processing, enzymolysis, centrifugal filtration, decoloration and deodorization, desalination, sterilization and spray drying. Compared with the yield less than 20% of soluble active ingredients such as the brown algae oligosaccharide prepared by a present biological enzyme method degraded kelp, the yield of the soluble kelp powder of the soluble kelp powder containing the brown algae oligosaccharide is 30-35% and is increased by 10-15%, wherein content of the brown algae oligosaccharide is 40-50%, and degree of polymerization is 2-7. Meanwhile, the processing step is simplified, the productivity is high, product quality is high, and a novel potential consumer market of the kelp is increased.

Description

technical field [0001] The invention relates to soluble kelp powder containing brown algae oligosaccharides and an enzymatic production method thereof, belonging to the technical field of marine resource processing. Background technique [0002] Kelp is a nutrient-rich brown algae plant that has certain medicinal value and is rich in a variety of active ingredients that are beneficial to the human body, such as mannitol, alginate, iodine, cellulose, vitamins, etc. These active ingredients are also medicinal Important raw materials for industries such as health care, chemical and agricultural fertilizers. Kelp is a very important marine plant resource in my country. The output and scale of kelp planting in China ranks first in the world, and it is a big country in kelp resources and comprehensive utilization. In addition to being used for direct consumption, kelp is one of the main raw materials of the brown algae industry. However, for many years, the development of kelp in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L17/60A23L17/00A23L5/40A23L5/20A23P10/40
CPCA23L5/273A23L5/40A23L17/60A23L17/65A23P10/40
Inventor 威廉·温蒂施江波刘晓勇陈静静张涛孟青王心怡
Owner 山东海之宝海洋科技有限公司
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