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Preparation method, product, and application of silk fibroin-based thixotropic hydrogel

A technology of silk fibroin and regenerated silk fibroin, which is applied in the field of preparation of thixotropic hydrogels, can solve problems such as poor therapeutic effect and large toxic and side effects, and achieve retention and controllability, low cost, and excellent touchability Effects of denaturation and viscoelasticity

Inactive Publication Date: 2019-10-08
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the shortcomings of the current traditional cancer chemotherapy methods such as poor therapeutic effect and large toxic side effects, this has brought great challenges to tumor treatment.

Method used

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  • Preparation method, product, and application of silk fibroin-based thixotropic hydrogel
  • Preparation method, product, and application of silk fibroin-based thixotropic hydrogel
  • Preparation method, product, and application of silk fibroin-based thixotropic hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of Thixotropic Hydrogels Containing Antitumor Functional Drugs and Photosensitizers

[0030] Silkworm cocoons are degummed with 0.5% sodium carbonate solution, dissolved in lithium bromide solution with a concentration of 9.3M, and dialyzed with deionized water to obtain a regenerated silk fibroin solution; then spread the regenerated silk fibroin solution on plastic culture In a dish, dry at least 24h at a temperature of 25°C and a relative humidity of 60% to obtain a silk fiber film; After soaking the silk fiber membrane in deionized water for 6 hours, centrifuge at a speed of 5000 rpm for 10 minutes, discard the undissolved silk fiber membrane to obtain a hydrophilic silk fibroin protein extract, and add doxorubicin to the extract (DOX) and Cy7 were left standing at 37° C. for 3 h to prepare a thixotropic hydrogel containing DOX and Cy7, wherein the concentrations of both DOX and Cy7 were 500 μg / mL.

[0031] (1) The thixotropic hydrogel containing DOX an...

Embodiment 2

[0039] Preparation of Thixotropic Hydrogels Containing Antitumor Functional Drugs and Photosensitizers

[0040] Silkworm cocoons are degummed with 0.5% sodium carbonate solution, dissolved in lithium bromide solution with a concentration of 9.5M, and dialyzed with deionized water to obtain a regenerated silk fibroin solution; then spread the regenerated silk fibroin solution on plastic culture In a dish, dry at least 24h under the conditions of 27°C and 70% relative humidity to obtain a silk fiber film; Soak the silk fibroin membrane in deionized water for 8 hours, centrifuge at 5500 rpm for 15 minutes, discard the undissolved silk fibroin membrane to obtain a hydrophilic silk fibroin extract, add paclitaxel and ICG to the extract After standing at 38°C for 6 hours, a thixotropic hydrogel containing paclitaxel and ICG was prepared, wherein the concentrations of paclitaxel and ICG were both 500 μg / mL, and the thixotropic hydrogel containing paclitaxel and ICG The field emissio...

Embodiment 3

[0042] Preparation of Thixotropic Hydrogels Containing Antitumor Functional Drugs and Photosensitizers

[0043] Silkworm cocoons are degummed with 0.5% sodium carbonate solution, dissolved in lithium bromide solution with a concentration of 10M, and dialyzed with deionized water to obtain a regenerated silk fibroin solution; then spread the regenerated silk fibroin solution on a plastic petri dish In the process, dry at least 24h at a temperature of 25°C and a relative humidity of 65% to obtain a silk fiber film; finally, the silk fiber film was mixed with a mass-volume ratio of 15:1 (mg:mL) to deionized water. After soaking the fibroin membrane in deionized water for 7 hours, centrifuge at a speed of 6000rpm for 12min, discard the undissolved silk fibroin membrane to obtain a hydrophilic silk fibroin extract, and add camptothecin and Ce6 to the extract Finally, after standing at 40°C for 1 h, a thixotropic hydrogel containing camptothecin and Ce6 was prepared, and the concent...

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Abstract

The invention relates to a preparation method, product and application of a silk fibroin-based thixotropic hydrogel, and belongs to the technical field of materials. A silk fibroin film is prepared, then immersed in water, and subjected to centrifugal treatment to discard undissolved silk fibroin film, and a hydrophilic silk fibroin extracting solution is obtained and left to stand. According to the preparation method, a natural silk is used as a raw material to prepare a thixotropic hydrogel, biosecurity is obtained, excellent thixotropic properties and viscoelasticity are obtained at the same time, pH, GSH, ROS, and NIR infrared light wave stimuli responsiveness are obtained, an anti-tumor drug is prepare by mixing the thixotropic hydrogel with one or more of an antitumor functional drugor a photosensitizer, and the antitumor drug has long-term retention and controllable drug release properties at the tumor site, the perfect combination of the stimuli responsiveness of the thixotropic hydrogel and photothermal therapy and photodynamic therapy can be realized, and the synergistic treatment of chemotherapy, photothermal, photodynamic is realized; and the thixotropic hydrogel is simple in preparation process, wide in source of raw materials, and low in cost, and the thixotropic hydrogel is suitable for expanding production.

Description

technical field [0001] The invention belongs to the field of material technology, and in particular relates to a preparation method, product and application of a silk fibroin-based thixotropic hydrogel. Background technique [0002] Malignant tumors have become one of the most important diseases that seriously threaten human health. According to the latest data, the overall cancer burden in China continues to rise. There are significant urban-rural and gender differences in the cancer burden. The cancer spectrum presents the coexistence of cancer spectrum in developed countries and cancer spectrum in developing countries. The situation of cancer prevention and control is very severe. Among them, the most common malignant tumors in China are lung cancer, gastric cancer, colorectal cancer and liver cancer, accounting for 76.7% of the incidence of all malignant tumors. However, due to the shortcomings of the current traditional cancer chemotherapy methods such as poor therapeu...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K47/42A61K31/704A61K31/4745A61K31/353A61K31/337A61K41/00A61P35/00
CPCA61K9/06A61K47/42A61K31/704A61K31/4745A61K31/353A61K31/337A61K41/0071A61P35/00A61K2300/00
Inventor 肖波苟双全
Owner SOUTHWEST UNIVERSITY
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