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Centellose, application of centellose as plant-derived preservative and application in cosmetics

A technology of Centella asiatica and polysaccharide, applied in cosmetics, cosmetic preparations, cosmetic preparations, etc., can solve problems such as no reports of antibacterial and fungal activity, and achieve no skin allergy and high skin safety. , excellent inhibitory effect

Active Publication Date: 2019-10-11
烟台新时代健康产业日化有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Centella asiatica polysaccharide is a macromolecular compound in Centella asiatica, and there is no report on its antibacterial and fungal activity

Method used

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  • Centellose, application of centellose as plant-derived preservative and application in cosmetics
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  • Centellose, application of centellose as plant-derived preservative and application in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Extraction and Antiseptic Performance Test of Centella Asiatica Polysaccharide

[0022] 1. Experimental materials

[0023] The whole herb of Centella asiatica was purchased from Bozhou Traditional Chinese Medicine Market, washed, air-dried, crushed and passed through a 60-mesh sieve for later use.

[0024] The tested bacteria include bacteria and fungi, wherein: bacteria include Escherichia coli, Proteus, Pseudomonas aeruginosa, Streptococcus, Staphylococcus aureus, and Bacillus subtilis, and fungi include Aspergillus niger and saccharomyces. Beef extract peptone medium was used for bacteria, potato dextrose agar medium was used for Aspergillus niger, and yeast extract peptone glucose agar medium was used for yeast.

[0025] 2. Experimental method

[0026] 1. Preparation and qualitative detection of Centella asiatica polysaccharide

[0027] Weigh the whole plant of Centella asiatica, use petroleum ether with a distillation range of 60-90°C, and 95% ethanol...

Embodiment 2

[0046] Embodiment 2: allergic test (patch test)

[0047] The test substance is the centella asiatica polysaccharide prepared in Example 1.

[0048] Referring to the "Safety and Technical Specifications for Cosmetics" (2015 edition), 30 healthy volunteers were selected as subjects, including 15 males and 15 females. After bathing, take 0.025 g of the test substance and place it in the small chamber of the spot tester, and apply the spot tester with the test substance on the back or the curved side of the forearm of the subject with a hypoallergenic adhesive tape, and gently press it with the palm of the hand to make it Apply evenly on the skin for 24 hours.

[0049] Observe the skin reaction according to the standards in Table 3 at 30min (after the indentation disappears), 24h and 48h after removing the test object spot tester, record the observation results and evaluate with reference to Table 3.

[0050] Table 3 Grading standard of skin reaction in skin-closed patch test

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Abstract

The invention discloses centellose, application of the centellose as a plant-derived preservative and application in cosmetics. The centellose is prepared following the steps that centella herb is weighed, subjected to reflux and degreasing by using petroleum ether and 95% ethyl alcohol in sequence, and filtered; after being subjected to solvent evaporation, filter residues are heated and extracted with distilled water, filtration is conducted, filter liquor is concentrated to leave one fifth of volume, filtration is conducted again, absolute ethyl alcohol with the volume being four times of the volume of the filter liquor is added, stirring is conducted to make the mixture uniform, standing for alcohol precipitation is conducted, and then suction filtration is conducted; precipitation iswashed with absolute ethyl alcohol, acetone and diethyl ether in sequence, redissolving is conducted by using distilled water, alcohol precipitation is repeated a time, deproteinization is conducted by using a Sevag method, and dialysis and freeze drying are conducted. An experimental result shows that the centellose has excellent inhibition activity to germs and fungi, the skin safety is high, skin allergy cannot be caused, the centellose can be used as the plant-derived preservative and then can be used for preservation of the cosmetics, and the centellose has the advantages of being natural, effective, mild and free of irritation.

Description

technical field [0001] The invention belongs to the field of cosmetics, and relates to the application of centella asiatica polysaccharide in cosmetics, in particular to a centella asiatica polysaccharide, its use as a plant source preservative and its application in cosmetics. Background technique [0002] Studies have found that natural antiseptic substances can be extracted from many kinds of plants in nature, which have a significant inhibitory effect on bacteria and / or fungi. These natural antiseptic substances include alkaloids, flavonoids, coumarins, saponins, etc. [0003] Because the side effects of natural antiseptic substances are generally small and relatively safe to the skin, the development of natural antiseptic plants from plants as preservatives and added to cosmetics has become a hot spot in the development of cosmetic preservatives. [0004] Centella asiatica is the dry whole herb of Centella asiatica, which contains a variety of active ingredients of tri...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K8/73A61Q19/00
CPCA61K8/73A61K2800/524A61Q19/00C08B37/0003
Inventor 姜东生
Owner 烟台新时代健康产业日化有限公司