Method for breeding jujube triploid by doubling induction of jujube female gamete chromosomes

A technology for chromosome doubling and female gametes, applied in the field of plant genetics and breeding, can solve problems such as treatment concentration, treatment time unrevealed, restricting development, and small jujube flowers.

Active Publication Date: 2019-10-25
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the small jujube flowers and the difficulty of artificial detasseling, the progress of cross breeding is slow
[0004] Although the use of female gamete chromosome doubling to breed triploids has been successfully applied in poplar, mulberry, Eucalyptus urophylla and other forest trees, there has been no report on the use of this method to breed triploids in jujube trees, mainly due to the induction There are some technical problems in jujube female gamete chromosome doubling that have not been effectively resolved, specifically: the effective treatment period for colchicine-induced jujube female gamete chromosome doubling has not yet been clarified, and the treatment concentration and treatment time have not yet been revealed. These problems have seriously restricted jujube Development of a triploid process by chromosome doubling in tree female gametes

Method used

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  • Method for breeding jujube triploid by doubling induction of jujube female gamete chromosomes
  • Method for breeding jujube triploid by doubling induction of jujube female gamete chromosomes
  • Method for breeding jujube triploid by doubling induction of jujube female gamete chromosomes

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Embodiment 1

[0022] Observe the diameter and shape of the flower buds of 'Ziziphus jujuba Mill. Mayazao', the external shape of the flower buds is as follows figure 1 As shown, the diameter of the flower bud is 1.76-2.12mm, and the shape is manifested as sepal gaps, yellow-green; figure 2 In the cotton soaking method shown, 0.3% colchicine is used to treat flower buds for 4 hours. After jujube flowers are freely pollinated to obtain seeds, seedlings are sown, and ploidy detection is performed with a loss cytometer to obtain triploid plants. , the triploid yield was 3.3%.

[0023] The cotton soaking method that adopts among the present invention is to punch the hole near the jujube hanger at the bottom of the jujube tree to the xylem, fill the hole with absorbent cotton, fix the centrifuge tube of 10ml bottom drilling on the absorbent cotton, and put 0.3% colchicum After the alkaline solution is added to the centrifuge tube, wrap it with tin foil, then wrap the centrifuge tube with parafi...

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Abstract

The invention discloses a method for breeding jujube triploid by doubling induction of jujube female gamete chromosomes and belongs to the field of genetic breeding of plants. The method includes thesteps of treatment of flower buds, pollination of jujube flowers, harvesting of seeds, seed sowing and seedling culture and ploidy identification. The method solves the key technical problem in the effective treatment period and the like in the doubling induction of jujube female gamete chromosomes by colchicine, builds a technical system for breeding triploid by doubling induction of jujube female gamete chromosomes, and lays a foundation for creating high-quality mltiploid germplasm and further development of jujube breeding process.

Description

technical field [0001] The invention relates to the field of plant genetics and breeding, in particular to a method for inducing jujube female gamete chromosome doubling and breeding jujube triploids. Background technique [0002] Jujube (Ziziphus.jujube Mill.) is a plant of the genus Ziziphus.Mill. in the family Rhamnaceae. It is an important woody fruit tree that originated in my country and has a long history of cultivation. Jujube fruit is delicious and rich in nutrients such as vitamin C, functional sugar, iron, calcium, potassium, zinc, etc., and has both edible and medicinal effects. [0003] Ploidy breeding is an important way of forest germplasm innovation. Among them, triploid has both ploidy effect and hybrid effect, and usually exhibits characteristics such as organ gigantism, sterility and vigorous secondary metabolism, and these characteristics are the main goals pursued by jujube breeding. At present, there have been some reports on jujube polyploid research...

Claims

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Application Information

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IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 李颖岳刘松珊侯璐李萌郭烨庞晓明
Owner BEIJING FORESTRY UNIVERSITY
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