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Streptomyces albus X-18 and method for producing epsilon-polylysine by using same

A technology of Streptomyces albicans and X-18, applied in the field of bioengineering, can solve the problem of low independent intellectual property rights, low production of ε-polylysine, late, Jiangnan University, Tianjin University of Science and Technology, Nanjing University of Technology, South China University of Technology And Nankai University and other issues, to achieve the effect of increasing production

Active Publication Date: 2019-10-25
SHENZHEN INNOVATION CENT OF SMALL MOLECULE DRUG DISCOVERY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on ε-polylysine was carried out earlier and relatively mature in Japan, and many strains with independent intellectual property rights have been obtained; related research in my country started relatively late, Jiangnan University, Tianjin University of Science and Technology, Nanjing University of Technology, South China University of Technology Universities and Nankai University and other units have done a lot of research on the screening, mutagenesis, molecular improvement breeding and fermentation production of ε-polylysine-producing strains, but there are few strains with independent intellectual property rights; There are few reports on the increase of ε-polylysine production stimulated by drugs

Method used

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  • Streptomyces albus X-18 and method for producing epsilon-polylysine by using same
  • Streptomyces albus X-18 and method for producing epsilon-polylysine by using same
  • Streptomyces albus X-18 and method for producing epsilon-polylysine by using same

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Describe how to screen and obtain the bacterial strain Streptomyces albicans ( Streptomyces albulus ) X-18

[0051] (1) Soil sample collection: Soil (soil samples were collected from Henan, Hebei, Jiangsu and other places) was sampled, and the soil at 5 cm below the surface of relatively dry and fertile soil was taken.

[0052] (2) Enrichment culture: 30 soil samples were collected, 2.5g of the samples were weighed and added to 25mL sterile enrichment medium, and incubated at 28°C and 150rpm for 2 days.

[0053] The composition of enrichment medium is (g / L): glycerol 10, agar 15, yeast extract 0.1, NaH 2 PO 4 0.68, MgSO 4 ·7H 2 O 0.25, (NH 4 ) 2 SO 4 0.66, 140mL trace mineral solution, pH 7.0, balance water.

[0054] The composition of trace mineral solution is (g / L): NaCl 1, FeSO 4 ·7H 2 O 0.1, ZnSO 4 ·7H 2 O 0.1, CaCl 2 2H 2 O 0.1, Alum 0.01, CuSO 4 ·5H 2 O 0.01, CoCl 2 ·6H 2 O 0.1, H 3 BO 3 0.01, NH 4 MO 2 4H 2 O 0.01, the balance is water. ...

Embodiment 2

[0058] Describe how to realize the Streptomyces albus ( Streptomyces albulus ) X-18

[0059] (1) Separation and purification of a single colony slant was submitted to Shanghai Bioengineering Co., Ltd. for sequencing, and the sequencing results were submitted to the NCBI database for comparison. The results showed that the 16S rDNA sequence homology between strain X-18 and Streptomyces albicans was as high as 98%. The phylogenetic tree of strain X-18 was constructed using MEGA 6.0 software image 3 , From the evolutionary distance analysis, it was found that X-18 was close to the standard type strain of Streptomyces albicans.

[0060] (2) According to the morphological characteristics, physiological and biochemical characteristics (see Table 1 below) and 16SrDNA characteristics of the strain X-18, the strain X-18 was determined to be Streptomyces albicans ( Streptomyces albulus ).

[0061]

[0062] Note: "+" indicates positive reaction; "-" indicates negative reaction. ...

Embodiment approach

[0066] Describe how to realize the raising of product epsilon-polylysine output, implementation method is as follows:

[0067] (1) Inclined solid culture

[0068] Inoculate the Streptomyces albicans CGMCC No. 17536 strain on a solid slant medium, and culture it at 26°C for 5 days to obtain the strains cultured on the slant solid, and set aside;

[0069] The formula of the solid slant medium is: for every 1000mL medium, add 2.0g of peptone, 7g of glucose, 1g of yeast powder and 15g of agar, pH 7.5, and the balance is water. ;

[0070] (2) Shake flask seed culture

[0071] Take the bacteria cultured on the slant solid prepared in the above step (1), add sterile water to make a bacterial suspension, and add 2 mL of sterile water according to one ring of bacteria; inoculate the bacterial suspension into the liquid seed medium , the inoculum volume was 5% of the volume of the liquid seed medium, the culture temperature was 26°C, the shaking table speed was 200 r / min, the pH was ...

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Abstract

The invention relates to a streptomyces albus X-18 and method for producing epsilon-polylysine by using the same, and belongs to the technical field of bioengineering. The streptomyces albus X-18 is separated and screened from soil and is preserved, and the preservation number is CGMCC No.17536. The streptomyces albus X-18 is used for producing the epsilon-polylysine, a fermentation culture mediumand fermentation conditions are improved, and intermediate material supplementation and a method for adding inducers are also used so that the fermentation yield of the epsilon-polylysine can reach 3.5-4.2 g / L. A strain with the independent intelligent property right is mainly used, and corn starch low in price is used as the main component of the fermentation culture medium, so that the production cost is reduced, and the fermentation yield is improved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a streptococcus albicans X-18 and a method for producing ε-polylysine by using the bacteria. Background technique [0002] Food is extremely vulnerable to microbial attack during storage, and the mildew and spoilage caused by it have a great impact on human life. According to incomplete statistics, about 20% of the food in the world is wasted due to mildew and corruption, and it also endangers people's health. Traditional anti-corrosion methods such as drying and salting will greatly change the flavor of the processed food. Therefore, adding food preservatives is still one of the effective means of food processing and preservation. During the use of synthetic food preservatives, people have gradually discovered that they have a great impact on human health. In recent years, the research and use of natural preservatives has gradually attracted people's attent...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P13/02C12R1/47
CPCC12N1/20C12P13/02C12N1/205C12R2001/47
Inventor 王大红徐鹏符丹丹郑迎莹沈文浩王梦洋孙建瑞原江锋古绍彬
Owner SHENZHEN INNOVATION CENT OF SMALL MOLECULE DRUG DISCOVERY CO LTD
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