Underwater transparent porous cellulose paper-based material for cell culture, and preparation method and application thereof

A technology for porous cellulose and cell culture, which is applied in the field of underwater transparent porous cellulose paper-based materials and their preparation, and can solve the problems of limiting the application of transparent cellulose paper, low resolution of cell images, and limiting the application of paper-based materials. , to achieve the effect of low cost, abundant sources, simple and effective preparation method

Active Publication Date: 2019-10-29
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, most of the traditional cell culture platforms (such as culture flasks and hydrogels) are transparent, and the paper-based materials themselves have poor light transmission, and cells cannot be observed when observed with an inverted microscope.
At the same time, when the later analysis is observed with a confocal microscope, due to the anisotropy of the fiber arrangement, the resolution of the cell image is not high, which limits the application of paper-based materials in cell culture.
In addition, an ideal cell culture support material should have sufficient porosity to allow the transport of nutrients and the discharge of metabolites, while the light transmittance and porosity of paper bases are often inversely proportional, thus limiting the use of transparent cellulose paper in cell culture. application

Method used

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  • Underwater transparent porous cellulose paper-based material for cell culture, and preparation method and application thereof
  • Underwater transparent porous cellulose paper-based material for cell culture, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Weigh 2 g of microcrystalline cellulose, add 20 mL of N,N-dimethylacetamide (DMAC), stir and activate at 130°C for 30 min, and then cool to room temperature. Add 6 g of lithium chloride and 80 mL of DMAC to the cooled cellulose, and heat and stir at 60° C. until the microcrystalline cellulose dissolves.

[0036] (2) Grinding sodium chloride (NaCl) with a mortar, and screening out NaCl particles with a size smaller than 45 μm.

[0037] (3) Take 3 mL of dissolved cellulose solution, add 2 g of ground NaCl particles, and stir evenly. Spread the mixture evenly on a Petri dish about 2mm, pre-gel at 40°C for 1h, then dissolve the solvent and NaCl in the solution with deionized water, the solvent exchange time is 20h; then freeze-dry at -20°C for 1h A porous cellulose paper base material is formed.

[0038] (5) The prepared cellulose paper-based material was sterilized in an autoclave at 120° C. for 20 minutes, and cut into discs with a diameter of 15 mm. 2 mL of gelati...

Embodiment 2

[0042](1) Weigh 3g of microcrystalline cellulose, add 30mL of N,N-dimethylacetamide (DMAC), stir and activate at 140°C for 45min, and then cool to room temperature. Add 7 g of lithium chloride and 70 mL of DMAC to the cooled cellulose, and heat and stir at 70° C. until the microcrystalline cellulose dissolves.

[0043] (2) Grinding sodium chloride (NaCl) with a mortar, and screening out NaCl particles with a size smaller than 45 μm.

[0044] (3) Take 3 mL of dissolved cellulose solution, add 3 g of ground NaCl particles, and stir evenly. Spread the mixture evenly on a petri dish about 3mm, pre-gel at 20°C for 1.5h, then dissolve the solvent and NaCl in the solution with deionized water, the solvent exchange time is 22h; then freeze-dry to form a porous Cellulose paper base material.

[0045] (5) The prepared cellulose paper-based material was sterilized in an autoclave at 140° C. for 25 minutes, and cut into discs with a diameter of 15 mm. 1 mL of 0.1% gelatin solution was ...

Embodiment 3

[0049] (1) Weigh 3 g of microcrystalline cellulose, add 25 mL of N,N-dimethylacetamide (DMAC), stir and activate at 150° C. for 1 h, and then cool to room temperature. Add 8 g of lithium chloride and 75 mL of DMAC to the cooled cellulose, heat and stir at 80° C. until the microcrystalline cellulose dissolves.

[0050] (2) Grinding sodium chloride (NaCl) with a mortar, and screening out NaCl particles with a size smaller than 45 μm.

[0051] (3) Take 3 mL of dissolved cellulose solution, add 3 g of ground NaCl particles, and stir evenly. Spread the mixture evenly on a Petri dish about 4mm, pre-gel at 60°C for 0.5h, then dissolve the solvent and NaCl in the solution with deionized water, and the solvent exchange time is 24h; then freeze-dry to form a porous Cellulose paper base material.

[0052] (5) Sterilize the prepared cellulose paper-based material in an autoclave at 120° C. for 20 min, cut it into discs with a diameter of 15 mm, add 1.5 mL of gelatin solution with a mass...

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Abstract

The invention relates to an underwater transparent porous cellulose paper-based material for cell culture, and a preparation method and application thereof. The preparation method comprises the following steps: firstly adding microcrystalline cellulose into an organic solvent A, carrying out stirring and preheating at 130-150 DEG C, then carrying out cooling to room temperature, then adding lithium chloride and an organic solvent B, and carrying out stirring at 60-80 DEG C until the microcrystalline cellulose is dissolved to obtain a cellulose solution; and adding a pore-forming agent insoluble in the organic solvent A and the organic solvent B into the cellulose solution, carrying out pre-gelling, then adding a solvent C capable of dissolving the pore-forming agent for solvent exchange, and after the pore-forming agent is completely dissolved in the solvent C, removing the solvent C and carrying out drying and post-treatment to obtain the underwater transparent porous cellulose paper-based material for cell culture. According to the invention, dissolution of the microcrystalline cellulose is effectively promoted through addition of lithium chloride, and the pore-forming agent is added, so that the porous cellulose paper-based material with different pore sizes can be obtained by controlling the size of the pore-forming agent, and high light transmittance is achieved while porosity is ensured.

Description

technical field [0001] The invention relates to the field of paper-based materials, in particular to an underwater transparent porous cellulose paper-based material for cell culture and its preparation method and application. Background technique [0002] In recent years, paper-based materials have attracted widespread attention as an emerging scaffold material for cell culture. Paper-based materials not only have good porosity, flexibility and biocompatibility, but also facilitate the adjustment of thickness and surface properties, as well as the ability to simulate extracellular matrix (ECM ), showing great potential as a cell culture platform. An outstanding advantage of the paper chip cell culture technology is that multiple paper-based units containing different cell types can be stacked to reproduce the 3D structure in the body, and can also be disassembled layer by layer without destroying the cell structure, simply realizing 3D-2D Transformation, from the spatial an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/26C08J9/28C08L1/04C12N5/00
CPCC08J9/26C08J9/28C08J2201/0446C08J2201/0484C08J2301/04C12N5/0075C12N2533/78
Inventor 唐蕊华姚雪刘丽娜张素风郑卓寅李菲
Owner SHAANXI UNIV OF SCI & TECH
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