Solid phase extraction method for various steroid hormones in saliva and method for detecting steroid hormones

Abstract

The invention discloses a solid phase extraction method for various steroid hormones in saliva. The method comprises activating a solid phase extraction column; adding the solid phase extraction column after a sample is processed; rinsing and eluting the solid phase extraction column, and collecting an eluent; adding an organic solvent and uniformly stirring the mixture, performing a centrifugal treatment at room temperature, and then transferring a product to a 96-orifice plate, sealing a film for a test. The solid phase extraction not only removes impurities, but also greatly improves the detection sensitivity and meets the detection requirement of trace steroid hormones in saliva. Compared with other pretreatment methods, the method is easy to operate, saves the solvent, has little impact on the environment, has a short analysis time and high flux, and can complete the test within 7 minutes. The method uses the isotope internal standard method to quantify the steroid hormones at a low detection error. It is verified by a comprehensive methodology that the method can be used for the detection of hydrogenated corticosterone, testosterone and dehydroepiandrosterone in saliva samples and screening of related diseases. The invention also discloses a method for detecting various steroid hormones after solid phase extraction of saliva.

Description

technical field [0001] The invention relates to the fields of steroid hormone solid-phase extraction and instrumental analysis mass spectrometry detection, in particular to a solid-phase extraction method of various steroid hormones in saliva and a detection method of the steroid hormones. Background technique [0002] With the rapid development of social economy, people's pace of life is accelerated, and the pressure is increased, which affects the secretion of corticosterone, testosterone, dehydroepiandrosterone and other steroid hormones in the body, and anxiety, depression, fear, sexual disorders, Infertility, premenstrual syndrome, sleep disturbance, cardiovascular disease and other diseases. Serum, urine and saliva are commonly used samples for testing human hormones. Since blood drawing itself is a source of acute stress, it may cause an increase in related steroid hormones, and most steroid hormones are combined with serum proteins and have no biological activity. Th...

AI Technical Summary

Problems solved by technology

[0005] 1. In addition to extracting steroid hormones, the liquid-liquid extraction method will also extract other substances similar in polarity to the extraction solvent, resulting in many impurities and large detection interference;

[0006] 2. The preparation of nano-hydroxyapatite-modified graphene oxide / polyvinylidene chloride composite microfiltration membrane requires tens of hours of processing, which is cumbersome and time-consuming;

[0007] 3. ELISA has poor anti-interference ability, prone to cross-reaction, and low sensitivity

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