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Ceriporia lacerate solid microbial inoculum for preventing and treating diseases and preparation method and application thereof

A technology for tearing cereus bacteria and solid inoculum, applied in the field of agricultural microorganisms, can solve the problems of poor degradability of vermiculite water-absorbing agent, unsustainable use, difficulty in storage of fermentation broth, etc., and achieves overcoming pesticide safety and environmental protection. The effect is not good, it is easy to store, and the effect of improving economic efficiency

Inactive Publication Date: 2019-11-05
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the defects of difficult storage, large volume, difficult transportation and secondary fermentation of the fermented liquid, it is difficult to popularize and apply
[0007] In 2018, the applicant's research team reported a formula for tearing the solid bacterial agent of the cereus cerevisiae in the 12th issue of the journal "Chinese Agricultural Sciences" (the quality of chaff, corn flour, vermiculite and water is 15:3:15:70 ratio), and the control effect and production increase effect of this solid bacterial agent on eggplant cotton blight, vermiculite is a kind of non-renewable resource that cannot be used continuously
In addition, vermiculite has poor degradability as a water-absorbing agent, and long-term use will cause reversible damage to the soil environment. In practical applications, it is found that the solid bacterial agent of the cultured cereus cereus on this substrate has poor stability and is harmful to agricultural land. Defects such as adverse effects

Method used

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  • Ceriporia lacerate solid microbial inoculum for preventing and treating diseases and preparation method and application thereof
  • Ceriporia lacerate solid microbial inoculum for preventing and treating diseases and preparation method and application thereof
  • Ceriporia lacerate solid microbial inoculum for preventing and treating diseases and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0029] The preparation of example 1 tearing cereus sp. HG2011 bacterial classification

[0030] 1. Strain activation: prepare 600ml PDB medium (PDB medium: 200g potato, 20g glucose, 1L water, pH 7.0) in a 1L Erlenmeyer flask, and sterilize at high temperature (121°C, 1.5kPa, sterilize for 30min); take and store At -20°C, the cereus spp. HG2011 strain was punched, and 12 bacterial blocks (Φ6mm) were inoculated in 600ml of PDB sterilization medium (2 bacterial blocks were inoculated according to 100ml); cultured at 28°C and 150rpm for 7 days, and the bacteria were obtained Kind of mother solution A (1ml>10 10 cfu).

[0031] 2. Seed liquid preparation: Prepare 35L seed medium (10g of corn flour passed through a 100 mesh sieve, 1g of yeast powder, 1L of water, pH6.5-7.0) in a 50L fermenter and sterilize; inoculate 600ml of strain mother solution A The sterilized seed culture solution was fermented by liquid (temperature: 27±1°C; stirring rate: 150rpm; ventilation volume: 10mL L ...

example 2

[0033] Example 2 The solid matrix screening test of the tearing cereus HG2011 with citrus peel residue as the main body

[0034] 1. Crop straw matrix screening test

[0035] Rice husks, corn stalks, wheat straws, rape stalks, rice straws and sorghum stalks were used as the main matrix (accounting for 98% by mass ratio), with 2% corn flour, the water content of the matrix was 60%, and the weight Ferment for 28 days with 5% cereus cerevisiae HG2011 solid microbial agent C (the same below). It was found that the treatment of 98% corn stalk or sorghum stalk + 2% corn flour had the highest number of viable bacteria of the cereus cleavens HG2011, which were 4.2*10 9 cfu / g, 4.5*10 9 cfu / g, considering the wide source and low price of corn stalks, corn stalks were selected as the raw material for blending citrus peels.

[0036] 2. Solid matrix screening test with citrus peel residue as the main raw material

[0037] Citrus peels are set at 5 levels, which are 60%, 65%, 70%, 75% an...

Embodiment 3

[0046] Embodiment 3 tearing cereus solid bacterial agent application test

[0047] 1. The effect of solid bacterial agent (CLA) on the yield and quality of cucumber

[0048] The experiment was carried out at the Experimental Farm of Southwest University (Xiama, Beibei District, Chongqing) from April to July 2018. The test soil is purple soil, medium soil, pH 7.3, organic matter 19.88g·kg -1 , total nitrogen 0.98g·kg -1 , total phosphorus 0.92g·kg -1 , total potassium 19.97g·kg -1 , Alkaline hydrolyzed nitrogen 60.60mg·kg -1 , available phosphorus 29.12mg·kg -1 , available potassium 53.49mg·kg -1 . The organic fertilizer is a commercial organic fertilizer (fresh base) with an organic matter of 30.01%, a nitrogen content of 1.38%, a phosphorus content of 1.15%, and a potassium content of 1.37%. Experimental settings (1) Control (CK): No fertilization, base application of 50kg / mu of autoclaved CLA; (2) Conventional fertilization (CF): application of organic fertilizer, ur...

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Abstract

The invention provide a ceriporia lacerate solid microbial inoculum, which comprises live bacteria and a solid substrate, wherein the live bacteria are ceriporia lacerate HG2011, and the live bacteriacount of the live bacteria is 3.8 * 10<8> cfu / g<-7>.1 * 10<9> cfu / g. The ceriporia lacerate HG2011 is characterized in that the solid microbial inoculum HG2011 has the effects of obviously promotinggrowth, increasing production and improving quality, and opens up a new growth-promoting and disease-preventing way.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to a method for producing a solid microbial agent of cereus cerevisiae by using citrus peel residues and an application thereof. Background technique [0002] The use of chemical fertilizers and pesticides in my country ranks first in the world. The high input of chemical fertilizers and pesticides has brought about a series of environmental, ecological and food safety problems. In 2018, the Ministry of Agriculture and Rural Affairs proposed to strive to achieve negative growth in the use of chemical fertilizers and pesticides by 2020, and weight loss and drug reduction were listed as a major research field for national scientific and technological development. Biocontrol bacteria mainly secrete antibacterial substances, compete with pathogenic bacteria for nutrient space, kill or inhibit pathogenic microorganisms, or induce plants to acquire resistance, improv...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P3/00A01G18/00
CPCA01G18/00
Inventor 袁玲隋宗明殷洁黄建国
Owner SOUTHWEST UNIVERSITY
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