Edible hippophae rhamnoides enzyme beverage and preparation method thereof
A seabuckthorn enzyme and beverage technology, applied in the direction of bacteria used in food preparation, application, food science, etc., can solve the problems of low efficiency and inability to maintain nutrients well
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[0022] In the present invention, the preparation method of the seabuckthorn enzyme stock solution comprises the following steps:
[0023] 1) Inoculating Lactobacillus plantarum and Leuconostoc enterococci into a mixture of seabuckthorn fruit and water, and performing the first non-heating fermentation at room temperature for 3 to 5 days to obtain the first fermentation liquid;
[0024] 2) Mix the first fermented liquid obtained in step 1) with rock sugar, inoculate Saccharomyces cerevisiae and Pichia pastoris, carry out the second normal temperature non-heating fermentation for 5-7 days, and obtain the second fermented liquid;
[0025] 3) Inoculate the second fermented liquid obtained in step 2) with Acetobacter pasteurianum, carry out the third non-heating fermentation at room temperature for 7-10 days, age for 3-6 months, and filter the supernatant to obtain the seabuckthorn enzyme stock solution.
[0026] The present invention inoculates Lactobacillus plantarum and Leuconos...
Embodiment 1
[0045] 1. Seabuckthorn enzyme stock solution: Add the pretreated seabuckthorn fruit to purified water in a mass ratio of 500g:1000mL into the enzyme fermenter, inoculate the activated Lactobacillus plantarum and Leuconostoc enterococci seed solution, the ratio of the two is 1 :1, the inoculum size is 5%, and the inoculum concentration is 1.0×10 6 CFU / mL, leave it to ferment at room temperature for 4 days; further add old rock sugar to fermentation broth in a mass ratio of 200g:1000mL into the enzyme fermenter, and then insert Saccharomyces cerevisiae and Pichia pastoris, the ratio of the two is 1:1, The inoculum volume was 4%, and the inoculum concentration was 1.0×10 6 CFU / mL, statically fermented at room temperature for 6 days; further inoculated with Acetobacter pasteurianus, the inoculum size was 8%, and the inoculum concentration was 1.0×10 9 CFU / mL, fermented on a shaking table at room temperature for 8 days, when the soluble solids content in the fermentation liquid wa...
Embodiment 2
[0050] 1. Seabuckthorn enzyme stock solution: Add the pretreated seabuckthorn fruit to purified water in a mass ratio of 500g:1000mL into the enzyme fermenter, inoculate the activated Lactobacillus plantarum and Leuconostoc enterococci seed solution, the ratio of the two is 1 :1, the inoculum size is 5%, and the inoculum concentration is 1.0×10 6 CFU / mL, leave it to ferment at room temperature for 4 days; further add old rock sugar to fermentation broth in a mass ratio of 200g:1000mL into the enzyme fermenter, and then insert Saccharomyces cerevisiae and Pichia pastoris, the ratio of the two is 1:1, The inoculum volume was 4%, and the inoculum concentration was 1.0×10 6 CFU / mL, statically fermented at room temperature for 6 days; further inoculated with Acetobacter pasteurianus, the inoculum size was 8%, and the inoculum concentration was 1.0×10 9 CFU / mL, ferment on a shaking table at room temperature for 8 days. When the soluble solids content in the fermentation liquid is 3...
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