Mesoporous titanium-silicon compound and application thereof in nucleic acid molecule detection

Abstract

The invention discloses a mesoporous titanium-silicon compound and an application of the mesoporous titanium-silicon compound in nucleic acid molecule detection. The mesoporous titanium-silicon compound is biomimetic synthesized by using a special nano structure of a yeast organism as a template and utilizing a biomineralization principle, can be used for preparing a biosensing electrode from an electrode sensing active material, is further used for detecting nucleic acid molecules, and has the advantages of high selectivity, good repeatability, simplicity and economy in operation and the like.

Description

technical field [0001] The invention belongs to the technical field of biosensing, in particular to a mesoporous titanium silicon compound and its application in the detection of nucleic acid molecules. Background technique [0002] Cancer is a major disease that threatens human life and health. According to the latest statistics from the National Cancer Center, about 7 people are diagnosed with cancer every minute. With early diagnosis and early treatment, cancer is easily curable. However, often due to the constraints of detection technology, once cancer is diagnosed, most of them are already in the middle and advanced stages, which brings great difficulties to the treatment of cancer. Therefore, early diagnosis and treatment of cancer is the key to cancer prevention and treatment. [0003] miRNA is a kind of non-coding, endogenous small molecule RNA (17-25 bases), which widely exists in animals and plants, and plays a key role in the occurrence and development of cance...

AI Technical Summary

Problems solved by technology

However, its relatively high cost and poor reproducibility require sufficient initial miRNA samples, which are generally only used for primary screening, and the results still need real-time fluorescent PCR verification

Real-time fluorescent quantitative PCR is often used in clinical and scientific research to detect miRNA. Although this method can sensitively detect low-abundance expressed miRNA and perform high-throughput screening, it is currently the most commonly used technology for detecting miRNA expression. However, the miRNA sequence is short and reversed. Difficult to record, complex primer design, strict temperature control

In addition, non-specific amplification during PCR can easily lead to false positives

[0005] In recent years, some new miRNA detection methods have emerged, involving technologies such as bioluminescence, enzyme analysis, molecular beacons, deep sequencing, locked nucleic acid, and bridging chain reaction, but these methods still have many insurmountable defects. For example, the specificity and sensitivity are relatively low, and the detection system introduces foreign molecular markers such as fluorescent indicators and enzymes, which not only increases the operation complexity and detection cost, but also introduces foreign errors, so it cannot meet the actual application requirements of miRNA detection.

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