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Preparation method of osteanagenesis material

A technology of bone regeneration and bone growth factor, applied in the field of biomedical engineering, can solve problems such as obstacles, and achieve the effect of improving the binding rate, maintaining the activity of promoting bone growth, and having a large load.

Active Publication Date: 2019-11-26
广州大鱼创福科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research on the use of these growth factors to treat bone injuries has been hampered by the inability to efficiently deliver these growth factors in a controlled manner with existing technologies

Method used

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  • Preparation method of osteanagenesis material

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preparation example Construction

[0025] The invention provides a method for preparing a bone regeneration material, comprising the following steps:

[0026] S1: Dissolving chitosan and polyethylene oxide completely in acetic acid solution, stirring evenly, to obtain spinning solution;

[0027] S2: using the spinning solution prepared in step S1 to perform electrospinning to obtain chitosan / polyoxyethylene nanofibers;

[0028] S3: washing the chitosan / polyoxyethylene nanofibers prepared in step S2 with DMEM medium to neutrality, after drying, they are activated by plasma treatment to obtain activated nanofibers;

[0029] S4: Soak the activated nanofibers prepared in step S3 in the DMEM medium containing recombinant bone growth factor and epigallocatechin gallate / carotenoid liposomes with a bath ratio of 1:100-300 to vacuum Negative pressure flash explosion at a temperature of 0.100-0.024mBar, followed by grafting reaction to obtain grafted nanofibers, wherein the epigallocatechin gallate / carotenoid liposomes ...

Embodiment 1

[0041] S1, with 0.5g chitosan (viscosity average molecular weight 5.0 * 10 5 , deacetylation degree is 80%) and 1.5g polyethylene oxide (average molecular weight 1.0×10 6 ) was completely dissolved in 100ml 90% (v / v) acetic acid solution, stirred evenly to obtain spinning solution;

[0042] S2. Electrospinning is carried out with the spinning solution, the syringe used is 10ml, the needle is flat, No. 7 needle; the electrospinning conditions are: voltage 15KV, distance 8cm, injection rate 0.5ml / h, temperature 30°C Obtain chitosan / polyoxyethylene nanofibers;

[0043] S3. Washing the chitosan / polyoxyethylene nanofibers with DMEM medium until the pH is 7, and then drying them at 37°C for 4 hours and then activating them with a plasma processor. The conditions for the plasma treatment are: the gas uses oxygen, and the processing power is 280W. The pressure is 55Pa, and the processing time is 15min;

[0044] S4. Dissolve 100mg of lecithin, 10mg of cholesterol and 3mg of astaxant...

Embodiment 2

[0048] S1, 0.25g chitosan (viscosity average molecular weight 5.0×10 5 , deacetylation degree is 80%) and 0.75g polyethylene oxide (average molecular weight 1.0×10 6 ) was completely dissolved in 100ml 90% (v / v) acetic acid solution, stirred evenly to obtain spinning solution;

[0049] S2. Electrospinning is carried out with the spinning liquid, the syringe used is 10ml, the needle is flat, and the needle is No. 7; the electrospinning conditions are: voltage 12KV, distance 7cm, injection rate 0.3ml / h, temperature 25°C Obtain chitosan / polyoxyethylene nanofibers;

[0050] S3. Washing the chitosan / polyoxyethylene nanofibers with DMEM medium until the pH is 7, and then drying them at 45°C for 2 hours and then activating them with a plasma processor. The conditions for the plasma treatment are: the gas is nitrogen, and the processing power is 250W. The pressure is 50Pa, and the processing time is 10min;

[0051] S4. Dissolve 100mg of lecithin, 10mg of cholesterol and 2mg of lyco...

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Abstract

The invention belongs to the field of biomedical engineering, and discloses a preparation method of an osteanagenesis material. The preparation method comprises the steps of (1) enabling chitosan andpolyoxyethylene to completely dissolve in a 90v / v% acetous solution, and performing uniform stirring to obtain spinning liquid; (2) performing electrostatic spinning with the spinning liquid to obtainchitosan / polyoxyethylene nanofibers; (2) washing the chitosan / polyoxyethylene nanofibers with a DMEM culture medium to be neutral, performing drying, then performing treatment with a plasma processorand performing activating; and (4) soaking activated nanofibers in the DMEM culture medium containing bone growth factors and epigallocatechin gallate ester / carotenoid liposome, performing negative pressure flash explosion, performing a grafting reaction, performing centrifugation and performing freeze-drying. According to the osteanagenesis material prepared by the preparation method, the bone growth healing speed can be effectively increased.

Description

technical field [0001] The invention relates to the field of biomedical engineering, in particular to a preparation method of a bone regeneration material. Background technique [0002] Platelet-derived growth factor (PDGF) and bone morphogenic protein-2 (BMP-2) are the two most important bone growth factors. As part of the natural wound-healing response, PDGF is the first factor released immediately after bone injury, such as a fracture. After PDGF, other factors, including BMP-2, help create the right environment for bone regeneration by recruiting osteogenic cells and forming a support structure, including blood vessels. Research on the use of these growth factors to treat bone injuries has been hampered by the inability to efficiently deliver these growth factors in a controlled manner. When very large quantities of growth factors are delivered too quickly, they are rapidly cleared from the treatment site, so they are less effective in tissue repair and can also cause ...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/18A61L27/50D01D5/00
CPCA61L27/18A61L27/20A61L27/50A61L2300/216A61L2300/414A61L2300/626A61L2400/12A61L2430/02D01D5/0038D01D5/0069D01D5/0092C08L5/08C08L71/02
Inventor 汤佳鹏葛彦龙朦朦朱俐
Owner 广州大鱼创福科技有限公司
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