Probiotics fermented functional food capable of warming meridians and dispelling cold and preparation method thereof
A functional food, probiotic technology, applied in biochemical equipment and methods, bacteria used in food preparation, microorganism-based methods, etc., to achieve unique taste effects
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Embodiment 1
[0045] Example 1: A functional food for warming meridian and repelling cold by probiotic solid-state staged fermentation and its preparation method
[0046] 1. Determination of raw material ratio
[0047] (1) Select high-quality lentils, soybeans, angelica, cinnamon, dried ginger, and fennel to grind them into powder and pass through a 100-mesh sieve;
[0048] (2) Preparation of activated medium: add 10g of soybean powder per 100mL of water; sterilization conditions: 121°C, 20min;
[0049] (3) Preparation of fermentation medium: mix lentil powder, soybean powder, angelica powder, cinnamon powder, dried ginger powder, and fennel powder in a certain proportion, and prepare a solid-state fermentation medium according to the mass ratio of the mixture and water at 1:1.5. Put it in a 6T solid-state fermenter for sterilization; sterilization conditions: 121°C, 20min;
[0050] (4) Strain activation: Lactobacillus reuteri, Bifidobacterium longum, Pediococcus lactis, Lactobacillus pla...
Embodiment 2
[0109] Example 2: Determination of bioactive components in functional food for warming meridian and expelling cold from probiotic solid-state staged fermentation
[0110] According to the type and sequence of the strains introduced into the functional food for warming meridians and dispelling cold through solid-state fermentation of probiotics, different metabolites are produced. There are more types of metabolites and higher activity, which can produce a synergistic effect and be more efficient. Good work.
[0111] ① After the probiotics were fermented in staged solid-state fermentation, Bacillus natto and Bacillus coagulans were introduced into them, and after 12 hours of aerated fermentation in an environment of 37°C, genistein, soybean isoflavone aglycone, mare phenol. The chromatographic column is Hypersil ODS2 (C18) 200mm×4.6mm, 5μm, the organic phase is methanol + acetic acid 0.1% (v / v), the aqueous phase is membrane-filtered water + acetic acid 0.1% (v / v), and the col...
Embodiment 3
[0118] Example 3: Blood enriching effect of probiotic solid-state staged fermentation of warming meridian and dispelling cold functional food
[0119] Cyclophosphamide (CTX) combined with acetylphenylhydrazine (APH) was used to establish a blood deficiency model in mice, and the thymus index (TI), spleen index (SI), red blood cell count (RBC), white blood cell count (WBC) PLT), hemoglobin count (HCT), and hematocrit (HGB) were observed indicators, and the blood enriching effect of probiotics solid-state staged fermentation of warming meridians and dispelling cold functional foods was compared. The results are shown in Table 7.
[0120] Table 7 The blood-enriching effect of probiotic solid-state staged fermentation warming meridian and dispelling cold functional food
[0121]
[0122] It can be seen from Table 7 that the warming meridian and dispelling cold functional food produced by the solid stage fermentation of probiotics has the effect of enriching blood. Peripheral b...
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