The present invention discloses meat pickling powder and a preparation method and a use method thereof. The meat pickling powder comprises corn starch, baking soda, papain, glucose and edible salt. The preparation method comprises the following steps of raw material inspecting and accepting, weighing, mixing and stirring, weighing, packaging and preserving. The use method comprises meat product pickling, pickling material preparing, meat product stirring, and cold storing. The meat pickling powder combines the various raw materials together, utilizes different functions of different accessory materials, can enable the pickled meat products to be fresh and tender, high in shaping degrees, and good in tasty degrees, overcomes the single function of single raw material, reduces the pickling steps, is wide in application ranges, can be used in pickling meat products of beef, mutton, pork, etc., can also be used to pickle edible materials of animal internal organs, etc., has a clear combination method of raw materials in parts by weight, and has theoretical basis and practical application supports.