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Callicarpa kwangtungensis polysaccharide as well as extraction method and application thereof

A technology of purple beads and polysaccharides, which is applied in the fields of medical formulas, medical preparations containing active ingredients, extracellular fluid diseases, etc., can solve problems such as abnormal hemostasis, bleeding diseases, and failure of blood coagulation factors to undergo biochemical processes, etc., and achieve good results. Social benefits and economic benefits, the effect of broad application prospects

Active Publication Date: 2019-12-10
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Normal people have a complete blood coagulation system, anticoagulation and fibrinolysis system, so the blood neither coagulates nor bleeds in the blood vessels, and always flows freely to complete its function. The link is abnormal, the coagulation factors in the plasma cannot undergo a series of biochemical processes, and finally fibrin clots cannot be formed, resulting in massive bleeding and bleeding disorders

Method used

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  • Callicarpa kwangtungensis polysaccharide as well as extraction method and application thereof
  • Callicarpa kwangtungensis polysaccharide as well as extraction method and application thereof
  • Callicarpa kwangtungensis polysaccharide as well as extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the preparation method of Guangdong Zizhu polysaccharide crude product (CKP)

[0031] Weigh 10 g of Guangdong Zizhu whole herb that has been crushed and passed through an 80-mesh sieve into an extraction container, add 5 times the volume of ethanol, reflux and degrease for 3 hours, remove the supernatant with 6 layers of gauze, and dry the sample. Put the dried sample in the reaction vessel, add 200-700ml of deionized water, and extract it with ultrasonic method. The ultrasonic extraction is specifically as follows: add deionized water according to the ratio of material to liquid 20-70mL / g, the extraction time is 20-70min, the extraction temperature is 20-70°C, and the ultrasonic power is 125-250W; after the extraction is completed, filter to obtain the extracted Concentrate the extract, add 4 times of ethanol to precipitate, place at 4°C for 24 hours, centrifuge to remove the supernatant, wash 3 times with absolute ethanol, acetone and ether, and dry in v...

Embodiment 2

[0043] Embodiment 2: the preparation method of Guangdong Zizhu homogeneous polysaccharide (CKP-1)

[0044] Add anhydrous petroleum ether to Guangdong Zizhu crude polysaccharide (CKP) solution (about 10mg / mL), stir and shake to make it milky, let it stand until the solution is layered up and down, and remove the petroleum ether layer; repeat the above operation 3-4 times , until no oily droplets appear in the petroleum ether layer, indicating that the degreasing is complete. Add Sevage reagent to the degreased polysaccharide solution, fully dissolve it, place it in a shaker, shake it, and centrifuge it to remove the denatured protein layer, and take the supernatant; repeat the above steps for 3-5 times on the supernatant until there is no white glue A deproteinized polysaccharide solution is obtained; add 4 times of ethanol for precipitation, place at 4°C for 24 hours, centrifuge to remove the supernatant, wash 3 times with absolute ethanol, acetone and ether, and vacuum dry A...

Embodiment 3

[0045] Embodiment 3: Determination of physicochemical properties of Guangdong Zizhu homogeneous polysaccharide (CKP-1)

[0046] 1. Determination of polysaccharide content

[0047] Accurately weigh an appropriate amount of the sample to be tested and dried to constant weight, and prepare a 0.1mg / mL polysaccharide solution. Use the sulfuric acid phenol method and the m-hydroxybiphenyl method to measure the total sugar and acid sugar content by spectrophotometry at 490nm and 525nm. Glucose calculation The total sugar content of Guangdong Zizhu polysaccharide crude product (CKP) is 52.82±3.78%, the acid sugar content is 10.66±1.29%, the total sugar content of Guangdong Zizhu homogeneous polysaccharide (CKP-1) is 89.93±3.87%, the acid sugar content is 12.63±2.45%.

[0048] 2. Determination of protein content

[0049] Precisely weigh 10 mg of the sample to be tested and dry it to constant weight, prepare it into a 1 mg / mL polysaccharide solution, and measure it. Use the Coomassie ...

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Abstract

The invention discloses callicarpa kwangtungensis polysaccharide as well as an extraction method and an application thereof. The callicarpa kwangtungensis homogeneous polysaccharide extracted and separated from callicarpa kwangtungensis comprises mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose in the molar ratio of 1:19.14:1.02:3.28:24.16:4.68:1.62; the relative molecular mass is 42.02 KDa; the polysaccharide content is 85% or higher, and the acidic sugar content is 10% or higher; and glucosidic bond types comprise T-alpha-arabinose, ->4-beta-galactose-(1->,->2,4-alpha-rhamnose-(1->,->4-alpha-galacturonic acid-(1->and->->4,6-alpah-glucose-(1->. The callicarpa kwangtungensis polysaccharide CKP-1 has good application prospects in preparation of procoagulant-related drugs.

Description

technical field [0001] The invention relates to a plant polysaccharide and its extraction method and application, specifically Guangdong Zizhu homogeneous polysaccharide CKP-1, its extraction method and application. Background technique [0002] Guangdong Zizhu is derived from the dry aerial part of Callicarpa kwangtungensis Chun, a plant of the Verbenaceae family. It is a species recorded in the "Pharmacopia of the People's Republic of China" (2015 Edition). Blood, hemoptysis, hematemesis, hematochezia, metrorrhagia, traumatic bleeding, lung-heat cough, sore throat, heat-toxin sores, water and fire burns, etc. Guangdong Zizhu has a wide distribution in China and is easy to cultivate. It is clinically used to treat gynecological diseases such as cervicitis, pelvic inflammatory disease, and vaginitis. The Chinese patent medicines that have been developed include Kanggongyan Tablets, Gongfukang Capsules, and Gongyanjing Tablets. . The preliminary research on the pharmacodyna...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P7/04
CPCA61K31/715A61P7/04C08B37/0003C08B37/006
Inventor 刘丽芳胡成李昊轩
Owner CHINA PHARM UNIV
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