Method for preparing ginsenoside Rg3 by adopting irradiation

A ginsenoside and irradiation technology, applied in chemical instruments and methods, glycoside steroids, steroids, etc., can solve the problems of low yield of Rg3, dehydration of panaxadiol group saponins, and limited industrial application, and achieve selective hydrolysis The effect of thorough, short catalytic time and complete selective hydrolysis

Active Publication Date: 2019-12-17
SICHUAN INST OF ATOMIC ENERGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. The physical method is generally carried out under high temperature and high pressure conditions, the processing time is long, and the separation and purification are cumbersome;
[0005] 2. Chemical method, using acid and alkali for conversion, it is easier to generate ginsenoside Rg by directional hydrolysis 3 , low cost, and easy to operate, but the reaction temperature generally needs to be controlled at 80-120°C, and ginsengdiol group saponins are prone to side reactions such as dehydration, displacement, and cyclization;
[0006] 3. Biological method, the reaction conditions are relatively mild, but the cost is high and the reaction conditions are harsh, which limits its industrial application
In this technical scheme, Rg 3 The yield rate is low (the highest is only 20.08-23.32%)

Method used

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  • Method for preparing ginsenoside Rg3 by adopting irradiation
  • Method for preparing ginsenoside Rg3 by adopting irradiation
  • Method for preparing ginsenoside Rg3 by adopting irradiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A kind of preparation ginsenoside Rg by irradiation 3 method, including the following steps:

[0049] A. Taking ginsengdiol group saponin as raw material, adding the aqueous ethanol solution of inorganic acid to obtain the ethanol solution of ginsengdiol group saponin-inorganic acid with a mass volume ratio of 1:5;

[0050] B. adding mass ratio to the ethanol aqueous solution of ginseng diol group saponin-inorganic acid is 0.5% irradiation auxiliary agent, stirs until completely dissolving;

[0051] C. the mixed solution obtained through step B is placed in the irradiation reactor, sealed, to 60 Co-γ ray is the irradiation source, with 0.5kGy / h as the irradiation dose rate, and irradiating 1kGy at room temperature; after purification by the adsorption column, the target ginsenoside Rg is obtained after detection. 3 Crude.

[0052] The irradiation reactor is made of glass or acid-proof and radiation-resistant plastic, and its volume can be set freely according to actu...

Embodiment 2

[0054] A kind of preparation ginsenoside Rg by irradiation 3 method, including the following steps:

[0055] A. Taking ginsengdiol group saponin as raw material, adding the aqueous ethanol solution of mineral acid, obtaining the ethanol solution of ginsengdiol group saponin-mineral acid with a mass volume ratio of 1:10;

[0056] B. in the ethanol aqueous solution of panaxadiol group saponin-inorganic acid, adding mass ratio is the irradiation aid of 5%, stirs until fully dissolving;

[0057] C. the mixed solution obtained through step B is placed in the irradiation reactor, sealed, to 60 Co-γ ray is the irradiation source, with 3kGy / h as the irradiation dose rate, and irradiating 15kGy at room temperature; after purification by the adsorption column, the target ginsenoside Rg is obtained after detection. 3 Crude.

Embodiment 3

[0059] A kind of preparation ginsenoside Rg by irradiation 3 method, including the following steps:

[0060] A. Take ginsengdiol saponins as raw material, add the ethanol aqueous solution of mineral acid, obtain the ethanol aqueous solution of the ginseng diol group saponin-mineral acid of mass volume ratio 1:7;

[0061] B. in the ethanol aqueous solution of panaxadiol group saponin-inorganic acid, adding mass ratio is the radiation aid of 2%, stirs until fully dissolving;

[0062] C. the mixed solution obtained through step B is placed in the irradiation reactor, sealed, to 60 Co-γ ray is used as the irradiation source, with 1.5kGy / h as the irradiation dose rate, 4kGy is irradiated at room temperature; after purification by the adsorption column, the target ginsenoside Rg is obtained after detection. 3 Crude.

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Abstract

The invention discloses a method for preparing ginsenoside Rg3 through irradiation, belonging to the technical field of medicine preparation. The method comprises the following steps: with panaxadiolsaponins as a raw material, pretreating the panaxadiol saponins with an ethanol aqueous solution of an inorganic acid, then adding an irradiation auxiliary agent, and irradiating the panaxadiol saponins with energy of 1-15 kGy at normal temperature by virtue of a <60>Co-gamma ray irradiation technology; and carrying out purifying with an adsorption column to obtain the target product ginsenoside Rg3. According to the method, high-energy rays are adopted for strengthening acid selective hydrolysis, and the yield of ginsenoside Rg3 prepared through irradiation is greatly increased compared withthe yield of ginsenoside Rg3 obtained through common acid hydrolysis; catalytic time is shorter; the usage amount of acid is obviously reduced; and the output and yield of the target ginsenoside Rg3 are effectively increased. It is guaranteed that the preparation method is easy to operate, low in cost and high in efficiency.

Description

technical field [0001] The invention relates to a method for preparing ginsenoside Rg 3 The method, especially relates to a kind of preparation ginsenoside Rg by irradiation 3 The method belongs to the technical field of medicine preparation. Background technique [0002] Ginsenoside is a sterol compound, a tetracyclic triterpenoid saponin, and is the main medicinal ingredient of Araliaceae Panax genus plants (such as: ginseng, Panax notoginseng and American ginseng, etc.). Modern pharmacological studies have shown that ginsenosides have various effects such as anti-aging, anti-oxidation, anti-fatigue, anti-inflammation, and enhancing immunity. "Research Progress on Ginsenoside Derivatization and Its Anti-tumor Structure-Activity Relationship, Cao Man et al." records: Ginsenosides have different degrees of anti-tumor activity, and the strength of its anti-cancer activity structure-activity relationship is: Protopanaxadiol type > protopanaxatriol type; aglycon > mono...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 张晓彬倪茂君陈浩彭朝荣陈竹平江力王静霞张婧刘思阳陈屿恒
Owner SICHUAN INST OF ATOMIC ENERGY
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