Method for preparing ginsenoside Rg3 by adopting irradiation

A ginsenoside and irradiation technology, applied in chemical instruments and methods, glycoside steroids, steroids, etc., can solve the problems of low yield of Rg3, dehydration of panaxadiol group saponins, and limited industrial application, and achieve selective hydrolysis The effect of thorough, short catalytic time and complete selective hydrolysis

CN110577561AActive Publication Date: 2019-12-17SICHUAN INST OF ATOMIC ENERGY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2019-12-17

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Abstract

The invention discloses a method for preparing ginsenoside Rg3 through irradiation, belonging to the technical field of medicine preparation. The method comprises the following steps: with panaxadiolsaponins as a raw material, pretreating the panaxadiol saponins with an ethanol aqueous solution of an inorganic acid, then adding an irradiation auxiliary agent, and irradiating the panaxadiol saponins with energy of 1-15 kGy at normal temperature by virtue of a <60>Co-gamma ray irradiation technology; and carrying out purifying with an adsorption column to obtain the target product ginsenoside Rg3. According to the method, high-energy rays are adopted for strengthening acid selective hydrolysis, and the yield of ginsenoside Rg3 prepared through irradiation is greatly increased compared withthe yield of ginsenoside Rg3 obtained through common acid hydrolysis; catalytic time is shorter; the usage amount of acid is obviously reduced; and the output and yield of the target ginsenoside Rg3 are effectively increased. It is guaranteed that the preparation method is easy to operate, low in cost and high in efficiency.
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Description

technical field

[0001] The invention relates to a method for preparing ginsenoside Rg 3 The method, especially relates to a kind of preparation ginsenoside Rg by irradiation 3 The method belongs to the technical field of medicine preparation. Background technique

[0002] Ginsenoside is a sterol compound, a tetracyclic triterpenoid saponin, and is the main medicinal ingredient of Araliaceae Panax genus plants (such as: ginseng, Panax notoginseng and American ginseng, etc.). Modern pharmacological studies have shown that ginsenosides have various effects such as anti-aging, anti-oxidation, anti-fatigue, anti-inflammation, and enhancing immunity. "Research Progress on Ginsenoside Derivatization and Its Anti-tumor Structure-Activity Relationship, Cao Man et al." records: Ginsenosides have different degrees of anti-tumor activity, and the strength of its anti-cancer activity structure-activity relationship is: Protopanaxadiol type > protopanaxatriol type; aglycon > mono...

Examples

Embodiment 1

[0048] A kind of preparation ginsenoside Rg by irradiation 3 method, including the following steps:

[0049] A. Taking ginsengdiol group saponin as raw material, adding the aqueous ethanol solution of inorganic acid to obtain the ethanol solution of ginsengdiol group saponin-inorganic acid with a mass volume ratio of 1:5;

[0050] B. adding mass ratio to the ethanol aqueous solution of ginseng diol group saponin-inorganic acid is 0.5% irradiation auxiliary agent, stirs until completely dissolving;

[0051] C. the mixed solution obtained through step B is placed in the irradiation reactor, sealed, to 60 Co-γ ray is the irradiation source, with 0.5kGy / h as the irradiation dose rate, and irradiating 1kGy at room temperature; after purification by the adsorption column, the target ginsenoside Rg is obtained after detection. 3 Crude.

[0052] The irradiation reactor is made of glass or acid-proof and radiation-resistant plastic, and its volume can be set freely according to actu...

Embodiment 2

[0054] A kind of preparation ginsenoside Rg by irradiation 3 method, including the following steps:

[0055] A. Taking ginsengdiol group saponin as raw material, adding the aqueous ethanol solution of mineral acid, obtaining the ethanol solution of ginsengdiol group saponin-mineral acid with a mass volume ratio of 1:10;

[0056] B. in the ethanol aqueous solution of panaxadiol group saponin-inorganic acid, adding mass ratio is the irradiation aid of 5%, stirs until fully dissolving;

[0057] C. the mixed solution obtained through step B is placed in the irradiation reactor, sealed, to 60 Co-γ ray is the irradiation source, with 3kGy / h as the irradiation dose rate, and irradiating 15kGy at room temperature; after purification by the adsorption column, the target ginsenoside Rg is obtained after detection. 3 Crude.

Embodiment 3

[0059] A kind of preparation ginsenoside Rg by irradiation 3 method, including the following steps:

[0060] A. Take ginsengdiol saponins as raw material, add the ethanol aqueous solution of mineral acid, obtain the ethanol aqueous solution of the ginseng diol group saponin-mineral acid of mass volume ratio 1:7;

[0061] B. in the ethanol aqueous solution of panaxadiol group saponin-inorganic acid, adding mass ratio is the radiation aid of 2%, stirs until fully dissolving;

[0062] C. the mixed solution obtained through step B is placed in the irradiation reactor, sealed, to 60 Co-γ ray is used as the irradiation source, with 1.5kGy / h as the irradiation dose rate, 4kGy is irradiated at room temperature; after purification by the adsorption column, the target ginsenoside Rg is obtained after detection. 3 Crude.