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Preparation method for preparing cysteine oligopeptide and membrane dressing through enzyme catalysis

A technology for the preparation of cysteine ​​oligopeptides and catalysis, which is applied in the field of enzyme-catalyzed preparation of cysteine ​​oligopeptides and the preparation of membrane dressings, can solve life-threatening problems, achieve less side reactions, mild reaction conditions, and high performance excellent effect

Pending Publication Date: 2019-12-24
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, accidental wounds such as burns or diseases cause skin damage, often triggering many local or systemic reactions, and large areas of skin damage can even be life-threatening.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Enzyme-catalyzed synthesis of cysteine ​​oligopeptides

[0023] a 1 1. Add 20 g of L-cysteine ​​methyl ester hydrochloride (Sinopharm Group Chemical Co., Ltd. Reagent Co., Ltd.), perfluoro-tert-butanol (Sinopharm Chemical Reagent Co., Ltd.) and 50 U bromelain (Shanghai Wokai Biotechnology Co., Ltd.), the pH value of disodium hydrogen phosphate-citric acid buffer is 6.0, perfluorinated The volume of tert-butanol is 15% of the volume of disodium hydrogen phosphate-citric acid buffer, and the reaction mixture was stirred (300 rpm) at 20°C for 12 hours;

[0024] b 1 , After the reaction finishes, the reaction mixture is filtered through an ultrafiltration membrane with a molecular weight cut-off of 5000, and the filtrate is concentrated through an ultrafiltration membrane with a molecular weight cut-off of 200;

[0025] c 1 , Remove the solvent by vacuum distillation at 30°C, the vacuum degree during vacuum distillation is controlled at 10pa, and the residual liquid ...

Embodiment 2

[0031] 1. Enzyme-catalyzed synthesis of cysteine ​​oligopeptides

[0032] a 1 1. Add 30 g of L-cysteine ​​methyl ester hydrochloride (Sinopharm Group Chemical Co., Ltd. Reagent Co., Ltd.), perfluoro-tert-butanol (Sinopharm Chemical Reagent Co., Ltd.) and 70 U trypsin (Shanghai Wokai Biotechnology Co., Ltd.), the pH value of disodium hydrogen phosphate-citric acid buffer was 8.5, perfluorinated The volume fraction of tert-butanol is 35% phosphate buffer, and the reaction mixture is stirred (200 rpm) at 30°C for 10 hours;

[0033] b 1 , After the reaction finishes, the reaction mixture is filtered through an ultrafiltration membrane with a molecular weight cut-off of 5000, and the filtrate is concentrated through an ultrafiltration membrane with a molecular weight cut-off of 200;

[0034] c 1 , Remove the solvent by vacuum distillation at 30°C, the vacuum degree during vacuum distillation is controlled at 5pa, the residual liquid is freeze-dried to obtain cysteine ​​oligopep...

Embodiment 3

[0040] 1. Enzyme-catalyzed synthesis of cysteine ​​oligopeptides

[0041] a 11. Add 40 g of L-cysteine ​​methyl ester hydrochloride (Sinopharm Group Chemical Co., Ltd. Reagent Co., Ltd.), perfluoro-tert-butanol (Sinopharm Chemical Reagent Co., Ltd.) and 100 U alkaline protease (Shanghai Wokai Biotechnology Co., Ltd.), the pH value of disodium hydrogen phosphate-citric acid buffer was 7, all The volume fraction of fluoro-tert-butanol is 40% phosphate buffer, and the reaction mixture is stirred (400 rpm) at 50° C. for 6 hours;

[0042] b 1 , After the reaction finishes, the reaction mixture is filtered through an ultrafiltration membrane with a molecular weight cut-off of 5000, and the filtrate is concentrated through an ultrafiltration membrane with a molecular weight cut-off of 200;

[0043] c 1 , Remove the solvent by vacuum distillation at 30°C, the vacuum degree during vacuum distillation is controlled at 1pa, the residual liquid is freeze-dried to obtain cysteine ​​oli...

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Abstract

The invention relates to a preparation method for preparing cysteine oligopeptide and membrane dressing through enzyme catalysis. The preparation method comprises the following steps of adding L-cysteine methyl ester hydrochloride, perfluoro-tert-butanol and proteases to a disodium hydrogen phosphate-citrate buffer solution, and performing a stirring reaction; filtering and concentrating a reaction mixture through an ultra filtration membrane; and performing vacuum distillation on filtrate after being concentrated through the ultra filtration membrane, and performing freeze drying to obtain the cysteine oligopeptide. The cysteine oligopeptide, diglycid ethyl ether and silver sulfadiazine are mixed, and coated to form the membrane so as to obtain a cysteine oligopeptide membrane dressing. The method has the advantages of being mild in reaction condition, environmental-friendly, small in side effects and the like, and can omit the steps of tedious protection and protection removal. The membrane dressing prepared by the method adopts the cysteine oligopeptide as a substrate, and the properties of microorganism resistance, biocompatibility, degradability and the like are excellent.

Description

technical field [0001] The invention relates to an enzyme-catalyzed preparation of cysteine ​​oligopeptides and a method for preparing membrane dressings thereof, belonging to the technical field of preparation of cysteine ​​oligopeptides. Background technique [0002] The skin is the largest organ of the human body, which can maintain the stability of the internal environment of the human body and prevent the invasion of harmful substances from the outside. However, accidental wounds such as burns or diseases cause skin damage, often triggering many local or systemic reactions, and large areas of skin damage can even be life-threatening. At this time, an excellent wound covering should be provided for the wound in time to protect the wound, while promoting wound healing, rebuilding and restoring skin function. The good biocompatibility and excellent biological performance of natural biopolymer materials have long been paid attention to and recognized by people, and they ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00A61L15/32A61L15/62A61L15/42
CPCC12P21/00A61L15/32A61L15/62A61L15/42
Inventor 蒋晓晓廖小凤尹韦蔚高鹏杨长林王峰
Owner JIANGNAN UNIV
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