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Seaweed active ingredient extraction method

An active ingredient and extraction method technology, applied in the field of biodegradation, can solve the problems of low total polysaccharide yield, cumbersome operation, long enzymatic hydrolysis time, etc., and achieve the effects of high polysaccharide total yield, shortened reaction time, and simple operation.

Inactive Publication Date: 2019-12-27
山东劲脉植物细胞信息技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The prior art has the following defects: (1) the extraction rate of sodium alginate is low; (2) the extraction rate of trehalan gum is low; (3) the total yield of polysaccharides is low; higher

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A kind of seaweed active ingredient extraction method

[0025] Step 1. Preprocessing

[0026] The seaweed used is Sargassum Gulf of Mexico, and the moisture content is 15-16%;

[0027] Now wash the Sargassum with fresh water, dry it at 90°C for 10 minutes, and grind it into 200-mesh fine powder after drying;

[0028] Add 3 times the mass of deionized water to the seaweed powder, stir evenly to obtain seaweed slurry.

[0029] Step 2. Enzyme treatment

[0030] Exoglucanase and propectinase are mixed according to a mass ratio of 1.5:1 to obtain the first enzymolysis mixture;

[0031] Mix the first enzymatic hydrolysis mixture and seaweed slurry at a mass ratio of 1:100 and add them to the reactor, adjust the reaction pH and reaction temperature, react for 19 hours, and the reaction temperature is 40°C. After the reaction is completed, keep warm for 5 minutes to obtain the first Enzymolyzate.

[0032] Protopectinase and papain are mixed in a ratio of 1:0.8 to obtain th...

Embodiment 2

[0038] A kind of seaweed active ingredient extraction method

[0039] Step 1. Preprocessing

[0040] The seaweed used is Fucus of the British Isles, and the water content is 14-17%;

[0041] Fucus is now washed with fresh water, dried at 90°C for 10 minutes, and ground into a 200-mesh fine powder after drying;

[0042] Add 3 times the mass of deionized water to the seaweed powder, stir evenly to obtain seaweed slurry.

[0043] Step 2. Enzyme treatment

[0044] Endoglucanase and lyase are mixed according to a mass ratio of 1.5:1 to obtain the first enzymolysis mixture;

[0045] Mix the first enzymatic hydrolysis mixture and seaweed slurry at a mass ratio of 1:125 and add them to the reactor, adjust the reaction pH to 5.0, adjust the reaction temperature, react for 20 hours, and the reaction temperature is 45°C. After the reaction is completed, keep warm for 5 minutes to obtain first enzymatic hydrolyzate.

[0046] Protopectinase and papain are mixed in a ratio of 1:0.9 to ...

Embodiment 3

[0052] A kind of seaweed active ingredient extraction method

[0053] Step 1. Preprocessing

[0054] The seaweed used is Northern Ireland deep-sea Ascophyllum nodosum, and the water content is 15-19%;

[0055] Fucus is now washed with fresh water, dried at 90°C for 13 minutes, and ground into a 200-mesh fine powder after drying;

[0056] Add 3 times the mass of deionized water to the seaweed powder, stir evenly to obtain seaweed slurry.

[0057] Step 2. Enzyme treatment

[0058] Cellobiase and polygalacturonase are mixed according to a mass ratio of 1.5:1 to obtain the first enzymolysis mixture;

[0059]Mix the first enzymatic hydrolysis mixture and seaweed slurry at a mass ratio of 1:100 and add them to the reactor, adjust the reaction pH to 5.5, adjust the reaction temperature, react for 21 hours, and the reaction temperature is 50°C. After the reaction is completed, keep warm for 5 minutes to obtain first enzymatic hydrolyzate.

[0060] Polygalacturonase and bromelain ...

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PUM

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Abstract

The invention provides an extraction method of seaweed, particularly relates to a seaweed active ingredient extraction method, and belongs to the technical field of biodegradation. The method includesmixing pretreated seaweed slurry with a first enzymolysis mixture to obtain a first enzymolysis product, mixing the first enzymolysis product with a second enzymolysis mixture to obtain a second enzymolysis product, mixing the second enzymolysis product with a third enzymolysis mixture to obtain a third enzymolysis product, and treating the third enzymolysis product to obtain a target product. Byadopting the seaweed enzymolysis treatment method disclosed by the invention, the extraction rate of sodium alginate is 38.0-50.9%; the extraction rate of the trehalose gum is 7.5-11.8%, the viscosity of the trehalose gum is high, the total polysaccharide yield is high, the enzymolysis reaction time of the seaweed is obviously shortened, the seaweed enzymolysis treatment method is simple to operate, and the cost is 30% lower than that of a conventional method.

Description

technical field [0001] The invention provides a method for extracting seaweed, in particular to a method for extracting active components of seaweed, which belongs to the technical field of biodegradation. Background technique [0002] The methods of extracting active ingredients from algae include chemical, physical and biological methods; the extracted active ingredients contain substances that regulate plant growth, including natural bioactive substances such as various minerals, amino acids and vitamins, which are beneficial to promote various physiological functions of plants. function. [0003] Seaweed extract contains active ingredients such as alginic acid, mannitol, chitosan, polypeptide, amino acid, cytokinin, betaine, auxin, seaweed polyphenol, abscisic acid, gibberellin, ethylene, polyamine, etc. Potassium, calcium, magnesium, iron, manganese, copper, zinc, boron, molybdenum and other large, medium and trace elements. [0004] The unique seaweed polysaccharides...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/04C07H3/04C07H1/08
CPCC07H1/08C07H3/04C08B37/0003C08B37/0084
Inventor 郭海山韩国成张秀花沈琼
Owner 山东劲脉植物细胞信息技术有限公司
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