Method for producing methane by anaerobic fermentation

An anaerobic fermentation and methane technology, applied in fermentation, climate change adaptation, organic fertilizer, etc., can solve the problem of low gas production rate of raw materials, achieve the effect of increasing methane production, promoting transformation, and improving the level of waste recycling

Active Publication Date: 2019-12-31
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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AI Technical Summary

Problems solved by technology

However, the metabolism of intermediate process products such as organic acids to methane is the bottleneck of efficient anaerobic conversion. How to overcome this bottleneck will greatly improve the anaerobic digestion of methane production in large-scale biogas projects. has major significance

Method used

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  • Method for producing methane by anaerobic fermentation
  • Method for producing methane by anaerobic fermentation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 Preparation of modified nano-iron oxide particles

[0023] (1) Preparation of magnetic nanoparticles with polyethyleneimine as a surface ligand molecule

[0024] FeCl 3 and FeCl 2 Dissolve in a certain amount of distilled water at a molar ratio of 2:1 and heat to 60°C. Under the protection of argon, concentrated ammonia water was slowly added to make the pH of the solution 9 and kept for 2 h. After the reaction, the sample was washed three times with deionized water and ethanol to obtain bare-core magnetic nanoparticles.

[0025] Take a certain amount of nano-Fe 3 o 4 Dissolve the magnetic particles in 100ml of distilled water, and ultrasonically treat them for 15-30 minutes to completely disperse them. Then slowly add 50-70 mg of surface ligand molecule polyethyleneimine into the solution. After stirring for 24-48 hours, the ligand-modified Fe 3 o 4 The nanoparticles are separated from the solution, washed repeatedly with distilled water and metha...

Embodiment 2

[0035] Embodiment 2 anaerobic fermentation produces methane

[0036] (1) Chopping the straw raw material into small sections of 1-5 cm for later use;

[0037] (2) get the mixture of anaerobic sludge and animal excrement as the inoculum for subsequent use;

[0038] (3) In the anaerobic digester, add 10-200 mg / L of straw raw material dry matter and inoculum, adjust the C / N ratio to 25:1-30:1, and add four kinds of magnetic nanoparticles prepared in Example 1, respectively. Granules 50-200 mg / L, and finally replenished with water until all materials in the entire anaerobic digester are 80% (v / v) of the effective volume of the reaction vessel, and the initial pH is adjusted to 7.0-7.6; among them, the dry matter of straw raw materials accounts for the above-mentioned 6-20% (g / 100mL) of the 80% effective volume, and the inoculum accounts for 20-60% (g / 100mL) of the above-mentioned 80% effective volume.

[0039] (4) Close the anaerobic digester, control the reaction temperature at...

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Abstract

The invention discloses a method for producing methane by anaerobic fermentation. Agricultural waste resources are utilized as raw materials for anaerobic fermentation to produce biogas, a small amount of magnetic nanoparticles are added to the fermentation system, and the magnetic nano particles are modified with nano-sized iron oxide particles on the surface so that the iron oxide particles havedifferent types of charges, which can promote the interaction between acidogenic bacteria and methanogenic bacteria so as to increase electronic transmission, and promote the transformation of organic acid to methane generation path so as to greatly increase the yield of methane. According to the present invention, nano-materials and agricultural waste resource utilization are combined, and a newapproach is opened for improving the level of waste resource utilization and breaking through the bottleneck of anaerobic fermentation technology.

Description

technical field [0001] The invention belongs to the field of anaerobic fermentation, and in particular relates to a method for producing methane by anaerobic fermentation. Background technique [0002] The anaerobic digestion process is widely used in the disposal of solid waste resources such as wastewater treatment, organic domestic waste, and agricultural, forestry and animal husbandry waste. The advanced point of anaerobic digestion is to convert organic carbon substances into methane, as a sustainable technology to realize energy recovery and utilization. However, the metabolism of intermediate process products such as organic acids to methane is the bottleneck of efficient anaerobic conversion. How to overcome this bottleneck will greatly improve the anaerobic digestion of methane production in large-scale biogas projects. has major significance. Contents of the invention [0003] The purpose of the present invention is to provide a method for producing methane by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C05F9/04
CPCC12P5/023C05F9/04C12P2203/00Y02A40/20Y02E50/30
Inventor 奚永兰叶小梅杜静靳红梅孔祥平王莉朱宁
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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